Method for preparing high-purity asiaticosid

A technology for asiaticoside and total asiaticoside is applied in the field of preparing high-purity asiaticoside, can solve problems such as unfavorable industrialized production, complicated process, excessive material consumption, etc., and achieves easy scale-up, industrialized production, and simple operation , the effect of large processing capacity

Inactive Publication Date: 2012-07-04
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The separation methods of asiaticoside in Centella asiatica mainly include silica gel column chromatography, macroporous resin adsorption, two-phase extraction, ultrafiltration membrane separation, supercritical CO 2 Fluid extraction, high-speed countercurrent chromatography, etc., these methods have certain shortcomings and are not conducive to industrial production
The main problems are: complex process, excessive material consumption, low yield, and small processing capacity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Weigh 500g of pulverized Centella asiatica powder, soak in 2500ml mass concentration of 75% ethanol aqueous solution, ultrasonically extract 4 times at 50°C, each time for 1 hour, filter, combine the filtrates and concentrate under reduced pressure to obtain 20.5g of extract; Dissolve the extract in 200ml of water, extract 3 times with an equal volume of petroleum ether in the aqueous extract solution, extract 4 times with an equal volume of ethyl acetate in the lower phase aqueous solution, and collect the lower phase aqueous solution. Use the same volume of n-butanol and ethyl acetate as the volume ratio of the lower phase aqueous solution to extract 3 times, combine the upper phase extracts, decolorize the extracts with activated carbon, and concentrate the decolorized extracts under reduced pressure to obtain a product. Madecassoside 7.3g; add 10 times the amount of methanol to dissolve asiaticoside, filter, concentrate the filtrate, and recrystallize with 90% ethano...

Embodiment 2

[0037] Weigh 500g of pulverized Centella asiatica powder, soak in 5000ml mass concentration of 75% ethanol aqueous solution, ultrasonically extract 4 times at 50°C, each time for 1 hour, filter, combine the filtrates and concentrate under reduced pressure to obtain 25.1g of extract; Dissolve the extract in 250ml of water, extract 3 times with an equal volume of petroleum ether in the aqueous extract solution, extract 4 times with an equal volume of ethyl acetate in the lower phase aqueous solution, and collect the lower phase aqueous solution. Use the same volume of n-butanol and ethyl acetate as the volume ratio of the lower phase aqueous solution to extract 6 times, combine the upper phase extracts, decolorize the extracts with activated carbon, and concentrate the decolorized extracts under reduced pressure to obtain a product. Madecassoside 9.3g; add 10 times the amount of methanol to dissolve asiaticoside, filter, concentrate the filtrate, and recrystallize with 90% ethano...

Embodiment 3

[0039] Weigh 500g of pulverized Centella asiatica powder, soak it in 7500ml mass concentration of 75% ethanol aqueous solution, ultrasonically extract 4 times at 50°C, each time for 1 hour, filter, combine the filtrates and concentrate under reduced pressure to obtain 28.7g of extract; Dissolve the extract in 290ml of water, extract 3 times with an equal volume of petroleum ether in the aqueous extract solution, extract 4 times with an equal volume of ethyl acetate in the lower phase aqueous solution, and collect the lower phase aqueous solution. Use the same volume of n-butanol and ethyl acetate as the volume ratio of the lower phase aqueous solution to extract 6 times, combine the upper phase extracts, decolorize the extracts with activated carbon, and concentrate the decolorized extracts under reduced pressure to obtain a product. Madecassoside 10.1g; add 10 times the amount of methanol to dissolve asiaticoside, filter, concentrate the filtrate, and recrystallize with 90% et...

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Abstract

The invention discloses a method for preparing high-purity asiaticosid. The method mainly comprises the following steps of: preparing an asiaticosid extracting solution by using an ethanol aqueous solution under an ultrasonic condition, and concentrating the extracting solution to obtain an extract; and extracting an extract solution with petroleum ether and ethyl acetate respectively to obtain an aqueous solution, extracting with a mixed solvent of n-butyl alcohol and ethyl acetate, combining upper phase organic solutions, decolorizing with active carbon, concentrated under reduced pressure, performing vacuum drying to obtain asiaticosid, and recrystallizing the asiaticosid to obtain high-purity asiaticosid. The process operation method has the advantages of easiness, no need of column chromatography separation in an operating process, short production period, large treating capacity, high product purity and suitability for industrial mass production.

Description

technical field [0001] The invention belongs to the technical field of extracting active ingredients from natural plants. Specifically, it is a method for preparing high-purity asiaticoside. technical background [0002] Centella asiatica (Centella asiatica (L) Urban) is the dry or rooted whole grass of the Umbelliferae plant Centella asiatica (L) Urban, also known as triptophylla, horseshoe grass, and Luodeda. It can be harvested all year round and is rich in resources. It grows in Southeast Asia , Australia, South Africa and the tropical regions of Central Africa, it has a long history of application in the field of medicine in many countries. Centella asiatica is cold in nature and bitter in taste. It has the effects of clearing away heat and dampness, detoxifying and reducing swelling. The chemical components in Centella asiatica mainly include asiaticoside, madecassoside, ginsenoside, asiatic acid, asiatica sugar, kaempferol, quercetin, etc. Recent studies have found...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 韦万兴唐新宗周敏凌秀荣
Owner GUANGXI UNIV
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