Chitosan-based styptic sponge and preparation method thereof
A hemostatic sponge and chitosan-based technology, applied in medical science, bandages, absorbent pads, etc., can solve the problems of poor thrombin stability, easy inactivation, and restrictions on the application of hemostatic sponges, and achieve good coagulation healing effect and excellent properties The effect of stabilizing and enhancing the healing effect of blood coagulation
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Embodiment 1
[0037] Chitosan was dissolved in distilled water to obtain a chitosan aqueous solution with a mass concentration of 0.5%; thrombin was dissolved in 0.1mol L of pH 7.0 -1 Phosphate buffer to obtain a thrombin solution with a mass concentration of 0.0015%, and then mix the two at a volume ratio of 3:1 to obtain 400 mL of a mixed solution with a weight ratio of chitosan and thrombin of 100:0.1. Under the condition of stirring, add 5g of glycerol and 10g of mannitol, and add 0.5mL of calcium chloride solution with a mass concentration of 5% dropwise, mix evenly, then pour into a mold with an appropriate shape, and place it for 2 hours until a transparent After gelation, freeze at -20°C for 24 hours, then vacuum freeze-dry at -40°C for 36 hours, mold, cut, and package. 60 The finished product is obtained after Co irradiation sterilization.
Embodiment 2
[0039] Chitosan was dissolved in distilled water to obtain a chitosan aqueous solution with a mass concentration of 2%; thrombin was dissolved in 0.1mol L of pH 7.0 -1 Phosphate buffer saline, obtain the thrombin solution that mass concentration is 0.06%, dissolve calcium chloride with distilled water, obtain the calcium chloride solution that mass concentration is 3%, then the three are mixed by volume ratio 3: 1: 0.1, Prepare chitosan, thrombin, Ca 2+410 mL of a mixed solution with a weight ratio of 100:1:1.8. Under the condition of stirring, add 4g glycerol and 20g mannitol, mix well. Then, under stirring, add 2.5 mL of glutaraldehyde solution with a volume concentration of 1% dropwise for cross-linking, then pour it into a mold with an appropriate shape, and let it stand for 2 hours. After forming a transparent gel, freeze it at -20°C for 24 hours. Then vacuum freeze-dry at -40°C for 36 hours, pour the mold, cut and package, 60 The finished product is obtained after Co ...
Embodiment 3
[0041] Chitosan was dissolved in distilled water to obtain a chitosan aqueous solution with a mass concentration of 2%; thrombin was dissolved in 0.1mol L of pH 7.0 -1 Phosphate buffer solution to obtain a thrombin solution with a mass concentration of 0.06%; dissolve the human-like collagen in distilled water to obtain a human-like collagen solution with a mass concentration of 2%, and then mix the three in a volume ratio of 3:1:60 Mix to prepare chitosan, thrombin, and human-like collagen in a weight ratio of 1:0.01:20 mixed solution 640mL. Under the condition of stirring, add 3.2g glycerol and 32g mannitol, mix well. Then, under stirring, add 2.5 mL of glutaraldehyde solution with a volume concentration of 0.1% dropwise for cross-linking, then pour it into a mold with an appropriate shape, and let it stand for 3 hours. After forming a transparent gel, freeze it at -20°C for 24 hours. Then vacuum freeze-dry at -20°C for 36 hours, pour the mold, cut and package, 60 The fini...
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