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Medicament composition containing ginsenoside and cantharidin and application of medicament composition

A technology of ginsenosides and total ginsenosides is applied in the directions of drug combinations, medical preparations containing active ingredients, active ingredients of heterocyclic compounds, etc., which can solve problems such as clinical application limitations, reduce the incidence of adverse reactions, and improve safety. , Improve the effect of anti-tumor effect

Active Publication Date: 2012-07-04
SHANGHAI GREEN VALLEY PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the clinical application of cantharidin and its extract and active ingredient cantharidin is greatly limited.

Method used

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  • Medicament composition containing ginsenoside and cantharidin and application of medicament composition
  • Medicament composition containing ginsenoside and cantharidin and application of medicament composition
  • Medicament composition containing ginsenoside and cantharidin and application of medicament composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: Preparation of total ginsenosides

[0045] 20kg ginseng, cut into small pieces of 1-2cm, add 160L 50% ethanol to reflux and extract twice, the first time for 3 hours, the second time for 1.5 hours, combine the extracts, centrifuge at 4500rpm for 20 minutes, take the supernatant and filter it with filter paper, collect The filtrate is used for later use.

[0046] Get 5kg of processed AB-8 resin, put it into a stainless steel chromatographic column with a diameter of 30cm, the diameter-to-height ratio of the resin bed is 1:10, wash with water, and filter the obtained ginseng extract at a rate of 1mL / min.cm 2 The sample was loaded twice at a high speed, and the static adsorption was performed for 12 hours. First wash 4BV with water and discard; then wash 3BV with 30% ethanol and discard; finally use 75% ethanol at 2-3mL / min.cm 2 Speed ​​elution, collect the eluate, concentrate and dry to obtain 50g of total ginsenosides.

Embodiment 2

[0047] Embodiment 2: The HPLC-ELSD pattern determination of total ginseng saponins

[0048] The total ginsenosides prepared in Example 1 were taken for HPLC-ELSD spectrum determination. Chromatographic column: Kromasil C18 (250mm×4.6mm, 5.0μm). Mobile phase: A is acetonitrile, B is water, gradient elution program: 0~10min, 5%A~20%A; 10~35min, 20%A; 10~35min, 20%A~29%A; 36~ 70min, 29%A; 70~100min, 47%A. Flow rate: 1mL·min-1; Column temperature: 35°C. Injection volume: 20 μL. Evaporative light scattering detector: T=40°C, P=0.35MPa, the carrier gas is high-purity nitrogen. Test results such as figure 1 shown.

[0049] Depend on figure 1 Visible, the total ginsenosides that the embodiment of the present invention 1 makes comprises ginsenoside Rf, Rb 1 , Rc, Rb 2 , Rd, Rg 1 , Re, 3-O-β-D-glucopyranosyl-oleanolic acid-28-O-β-D-glucopyranoside, notoginsenoside NR 4 , 3-O-α-L-arabinofuranosyl (2-1)-α-L-rhamnopyranose (3-1)-β-D-glucopyranose-(4-1)-β-D -Glucopyranosyl--Olea...

Embodiment 3

[0052]Embodiment 3: the preparation of mylabris extract

[0053] Weigh 20g of mylabris powder, pour it into a 1000mL round bottom flask, add 250mL of acetone, shake intermittently at 30°C for 2h, and extract twice. The extracts were combined, concentrated under reduced pressure, washed with 95% ethanol:petroleum ether (1:1.5) until the washing liquid was colorless, and a near-white crude product was obtained, then about 5mL of acetone was added, heated to dissolve, filtered while hot, and the filtrate was placed at 4°C Natural crystallization gave 50 mg of white transparent needle crystals, yield 0.25%. The product was detected by liquid chromatography and gas chromatography, and no impurity peaks were found, indicating that the product had a purity greater than 99.5%.

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Abstract

The invention relates to the field of Chinese medicine, and discloses a medicament composition containing ginsenoside and cantharidin and application of the medicament composition. The medicament composition containing the ginsenoside and the cantharidin contains the cantharidin or a Chinese medicinal material extract containing the cantharidin and the ginsenoside or a Chinese medicinal material extract containing the ginsenoside, wherein the ginsenoside or the Chinese medicinal material extract containing the ginsenoside is enough to reduce the toxic or side effect produced by the cantharidin. According to the medicament composition containing the ginsenoside and the cantharidin, the toxicity of the cantharidin on the kidney is obviously weakened, the adverse effect rate is obviously reduced, and the using safety of the cantharidin is greatly improved; and the medicament composition has the effect of improving tumor resistance, is safe in clinical use and low in toxic or side effects, and can be widely applied to preparation of medicaments for treating various cancers.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to a pharmaceutical composition containing ginsenoside and cantharidin and its application. Background technique [0002] Mylabris, also known as Mylabris phalerata Pallas or Mylabris cichorii Linnaeus, is the dry body of Mylabris phalerata Pallas or Mylabris cichorii Linnaeus. Catch in summer and autumn, suffocate or burn to death, and dry in the sun. Mylabris is a kind of precious Chinese medicinal material. Li Shizhen, a great medical scientist in the Ming Dynasty, said in the masterpiece "Compendium of Materia Medica" that mylabris is pungent in nature, cold and poisonous. , breaking stones, blood accumulation, hurting human muscles, treating scabies, abortion, treating scrofula, clearing waterways, treating gonorrhea, treating malignant sores, fistulas, treating hernia, detoxifying boils, and treating rabies. [0003] Cantharidin mainly contains cantharidin, monote...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61K35/64A61K31/704A61P35/00A61K31/34
Inventor 刘艳丽许琼明陈重刘江云何文君贾丹张苗苗杨世林
Owner SHANGHAI GREEN VALLEY PHARMA
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