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Separation and purification method for dendrobium polysaccharide

A technology for the separation and purification of dendrobium polysaccharides, which is applied in the field of separation and purification of dendrobium polysaccharides, can solve problems such as lack of innovation, and achieve the effects of preserving the biological activity of dendrobium polysaccharides, increasing the extraction rate, and increasing the extraction rate

Active Publication Date: 2012-06-20
云南金九地生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The polysaccharide extraction methods in most patents only include a few routine steps: degreasing, water extraction, alcohol precipitation, protein removal, and drying; lack of innovation, and the extracted polysaccharide content is mostly 20%-25%

Method used

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  • Separation and purification method for dendrobium polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The dried dendrobium sample was pulverized with a pulverizer, sieved, and 100 g of 80 mesh dendrobium powder was taken. Reflux twice with 15 times of 95% ethanol, filter, and place in a cool and ventilated place to volatilize the organic solvent to obtain medicinal residues.

[0027] Microwave-assisted solvent extraction, microwave time 75 seconds, microwave intensity 800W, add 15 times the mass of distilled water.

[0028] Add 2% cellulase, adjust the pH of the solution to 6.0, and keep the water temperature at 40°C for 2 hours of enzymatic hydrolysis.

[0029] Filter with double-layer filter cloth, extract the filter residue with 20 times distilled water, the temperature of the water bath is 90°C, and extract twice with water, the first time is 2 hours, and the second time is 1 hour. Suction filtration, discard the residue, collect the suction filtrate, and combine the suction filtrate to obtain the extract.

[0030] Concentrate the extract in vacuum to 1 / 6 of the o...

Embodiment 2

[0032] The dried dendrobium sample was pulverized with a pulverizer, sieved, and 100 g of 80 mesh dendrobium powder was taken. Reflux twice with 10 times of absolute ethanol, filter, and evaporate the organic solvent to obtain medicinal residues. Microwave-assisted solvent extraction, microwave time 75 seconds, microwave intensity 800W, add 15 times the mass of distilled water. Filter with double-layer filter cloth, add 2% cellulase, adjust the pH value of the solution to 6.0, and keep the water temperature at 40°C for 2 hours for enzymatic hydrolysis. Filter with double-layer filter cloth, extract the filter residue once with 30 times distilled water, and the temperature of the water bath is 100°C; after the water extraction is completed, filter with suction, collect the filtrate to obtain the extract.

[0033] The extract was concentrated in vacuum to 1 / 6 of the original volume, and 95% ethanol was added until the ethanol concentration reached 80%. Alcohol precipitation at...

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Abstract

The invention relates to a separation and purification method for dendrobium polysaccharide. The process steps comprise: raw material selecting, crushing, screening, degreasing, microwave assisted solvent extraction, enzyme extraction, filtering, water extraction, suction filtration, vacuum concentration, alcohol precipitation, centrifugal separation, freeze drying, crude product obtaining, protein removing, freeze drying, and dendrobium polysaccharide obtaining. The technical effect of the present invention is that: the extraction rate of the dendrobium polysaccharide is improved, the extraction time is shortened, the energy consumption is reduced, and the biological activity of the dendrobium polysaccharide is effectively maintained.

Description

technical field [0001] The invention relates to a method for separating and purifying dendrobium polysaccharides. Background technique [0002] Dendrobium is one of the largest genus of Orchidaceae, with more than 1500 species, widely distributed in tropical and subtropical regions such as Asia, Europe and Oceania. There are about 80 species in my country, mainly distributed in Southwest, East and South China. In traditional Chinese medicine, the fresh or dried stems of various plants of the genus Dendrobium are harvested for medicinal purposes, collectively referred to as Dendrobium. Dendrobium officinale is a traditional Chinese medicinal material in my country. Modern Chinese medicine theory believes that Dendrobium officinale has the functions of strengthening the spleen and stomach, nourishing yin and kidney, moistening the lungs and promoting body fluid, etc. It is used to treat chronic atrophic gastritis, hypertension, diabetes, anti-tumor, and anti-aging. For deca...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 崔武卫聂少平黄晓君谢明勇田芳
Owner 云南金九地生物科技有限公司
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