Sample introduction method and sample introduction device for microfluidics detection of fish-farming water environment

Abstract

The invention discloses a sample introduction method and a sample introduction device for microfluidics detection of a fish-farming water environment. Two ends of a tank body are respectively connected with an A end extrusion pump and a B end extrusion pump, and a 10 mum level filter screen is arranged in a space between the A end extrusion pump and the B end extrusion pump in a cavity of the tank body. A sample introduction channel is connected with a microfluidics device outside the tank body, the top of the tank body is connected with a magnetic stirring and pumping pressure conveying structure through a sample channel, an original water sample in the fish-farming water environment can be automatically and fully stirred, cluster materials including cenobium and the like in the water sample can be automatically dispersed, and extrusion pumps at two ends are respectively extruded according to a certain sequence. After the dispersed sample is automatically guided in through a push type sample introduction mode, the introduced sample is filtered, the processes from sample dispersing, sample filtering, sample introduction to the filter screen cleaning can be completed in a full automation mode, the microfluidics detection is guaranteed to be carried out smoothly, and the degree of automation is high.

Description

technical field [0001] The invention relates to fish breeding technology, in particular to a method and a device for detecting fish breeding water environment by using microfluidic control. Background technique [0002] The water environment of fish farming is an important factor affecting the production of aquaculture. Poor water quality environment will affect the reproduction and growth of fish. In severe cases, it will cause a large number of harmful bacteria to breed in the water environment, causing large-scale fish diseases and the death of bacterial diseases. The rate is often as high as 70%~80%, causing huge economic losses. Therefore, the real-time monitoring of the fish farming water environment has become the key to the prevention and treatment of fish diseases and bacteria in the fish farming industry. Patent application number 200410041911.5 discloses a fish disease automatic diagnosis method and its device using image processing. The method applies computer d...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a microfluidic detection method in the water environment for fish farming to solve the problems that the introduction of samples in the microfluidic detection in the current fish farming water environment may easily cause the blockage of the microfluidic chip and the occurrence of fish diseases cannot be accurately detected. The advanced sampling method and the automatic sampling device with sample pretreatment adopt an adsorption push-pump filter structure for the particularity of the fish culture water environment, which integrates the sample pretreatment and sampling links into one, greatly improving micro Efficiency of flow control detection

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