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Volume dissolved oxygen transfer coefficient staged control method based on citric acid metabolic network

A control method and metabolic network technology, applied in the field of fermentation process optimization, can solve problems such as reduced acid production and prolonged fermentation time, and achieve the effects of low equipment requirements, increased production, and low energy consumption

Inactive Publication Date: 2012-05-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the temperature is too high, the bacteria will proliferate in large quantities, and the sugar will be consumed in large quantities so that the acid production will be reduced, and more oxalic acid and gluconic acid will be produced at the same time; if the temperature is too low, the fermentation time will be prolonged

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Determination method of citric acid content:

[0024] Mobile phase: 0.5% H3PO4 / KH2PO4 buffer (PH=2.81); containing 0.025% acetonitrile Flow rate: 1.0ml / min

[0025] Chromatographic column: ODS reversed phase column

[0026] Column temperature: 40°C

[0027] Detection wavelength: 206nm

[0028] Injection volume: 5ul

specific Embodiment 2

[0029] Dissolve 291g of corn flour in 1L of water, add lime to adjust the pH to 6.0-6.4, then add amylase, keep warm at 100°C for 30 minutes, then cool down to 60°C, add glucoamylase, keep warm until the iodine test is brown or brown-red, then When the pH is about 5.6 or so.

[0030] The amylase is derived from the amylase commonly used in the market, and the amount added during the liquefaction process is adjusted according to the enzymatic properties of the enzyme and the substrate, and it comes from Genencor.

[0031] The glucoamylase is derived from commonly used glucoamylase on the market, and the amount added during the liquefaction process is adjusted according to the enzymatic properties of the enzyme and the substrate, and it comes from Novozymes.

[0032] The liquefied raw material liquid is subjected to plate and frame pressure filtration or centrifugation to obtain a filtrate.

specific Embodiment 3

[0033] Solid Culture: Potato Medium (PDA)

[0034] Solid culture conditions: 35°C, 8 days

[0035] Seed culture medium: the filtrate obtained from the liquefaction of the above-mentioned corn flour controls the total sugar at about 9.5%, 13m 3 The mixed liquid is fixed to 26m 3

[0036] Seed culture conditions: 37°C, 125rpm / min, culture for 24 hours, the inoculum size is one eggplant bottle connected to 26m 3

[0037] Fermentation medium: according to every 39m 3 Add 1.6 tons of corn flour in the clear night, add 5m 3 water calculation

[0038] Fermentation culture conditions: inoculum size 8%, 26m 3 The seed solution was inoculated to 340m 3 In the fermentation tank, the temperature is maintained at 37°C, the stirring speed is 84rpm / min, the tank pressure is 57kp, and the ventilation rate is controlled at 40m for 2 to 3 hours in the early stage of fermentation. 3 / min, about 3~36h in the middle stage of fermentation) maintained at 48m 3 / min or so, from 36h in the l...

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Abstract

The invention discloses a volume dissolved oxygen transfer coefficient staged control method based on a citric acid metabolic network. The metabolic flow direction is controlled through controlling the dissolved oxygen of citric acid at different fermentation stages, so the yield of the citric acid is improved. In the whole fermentation process of the citric acid, seed liquid is inoculated into a fermentation tank at the inoculation quantity of 8 percent, the temperature is maintained at 37 DEG C, the tank pressure is 0.05 MPa, the stirring speed is maintained at 84 rpm / min, and the air introduction quantity is controlled to be maintained to be 40m<3> / min in the fermentation beginning period, about 48m<3> / min in the fermentation medium period (the fermentation is started for about 2 to 3 hours) and 40m<3> / min in the fermentation later period. When the fermentation is carried out for about 58 hours, the reducing sugar content is detected, and the fermentation can be stopped when the reducing sugar content is reduced to a value lower than 5 percent. The dissolved oxygen transfer coefficient staged control is realized through optimizing the air introduction quantity at different fermentation stages, the improvement for improving the citric acid yield based on the metabolic network is realized, the process is simple and convenient and is easy to operate, the requirement on equipment is low, the energy consumption is little, and the method can be widely applied to citric acid production.

Description

technical field [0001] The invention relates to a stepwise control method for volume dissolved oxygen transfer coefficient based on a citric acid metabolic network, and belongs to the field of fermentation process optimization. Background technique [0002] Citric acid is an important organic acid, also known as citric acid, a colorless crystal, often containing a molecule of crystal water, odorless, has a strong sour taste, easily soluble in water, and can be ionized with 3 H+; heating can It decomposes into various products and reacts with acids, alkalis, glycerin, etc. Its calcium salt is more soluble in cold water than hot water, and this property is often used to identify and separate citric acid. Anhydrous citric acid can be obtained by controlling the appropriate temperature during crystallization. [0003] Because citric acid has a mild and refreshing sour taste, it is widely used in the manufacture of various beverages, soft drinks, wine, candies, snacks, biscuits...

Claims

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Application Information

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IPC IPC(8): C12P7/48C12R1/685
Inventor 陈坚堵国成刘龙李江华
Owner JIANGNAN UNIV
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