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Preparation method of collagen sponge

A collagen sponge and collagen technology, which is used in medical science, absorbent pads, prostheses, etc., can solve the problems of short degradation time and poor mechanical properties, and achieve high protein content, good tissue repair performance, and good biocompatibility. performance effect

Active Publication Date: 2012-04-18
北京益而康生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of collagen sponges currently on the market are poor mechanical properties and short degradation time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Clean the purchased qualified raw material Achilles tendon with deionized water, cut the Achilles tendon to a suitable size with a slicer and put it in an enzymatic hydrolysis tank for later use. Add water for injection to 1000g of bovine Achilles tendon, add an appropriate amount of acetic acid to adjust the pH to 1.0, add pepsin for enzymolysis, the concentration of pepsin is 0.01mg / L, and the temperature is 0°C for 120h. After the enzymolysis is completed, put the solution in the enzymolysis tank into a centrifuge and centrifuge at a speed of 1000r / min for 120min. Take the supernatant. Pour the centrifuged supernatant into a stainless steel barrel, add saturated sodium chloride solution into the stainless steel barrel, the volume ratio of the saturated sodium chloride solution to the supernatant is 1:1, and keep stirring until all the collagen is precipitated. Put the salted-out collagen into a dialysis bag and put it into a dialysis tank for dialysis for 5 days. P...

Embodiment 2

[0024] Clean the purchased qualified raw material Achilles tendon with deionized water, cut the Achilles tendon to a suitable size with a slicer and put it in an enzymatic hydrolysis tank for later use. Add water for injection to 1000g bovine Achilles tendon, add an appropriate amount of citric acid to adjust the pH value to 5.0, add trypsin for enzymolysis, the concentration of trypsin is 1000mg / L, and the temperature is 30°C for 12 hours. After the enzymolysis is completed, put the solution in the enzymolysis tank into a centrifuge and centrifuge at a speed of 10000r / min for 12min. Take the supernatant. Pour the supernatant after centrifugation into a stainless steel bucket, add sodium potassium chloride and solution into the stainless steel bucket, the volume ratio of the saturated sodium chloride potassium solution to the supernatant is 1:100, and keep stirring until the collagen is completely precipitated . Put the salted-out collagen into a dialysis bag and put it into...

Embodiment 3

[0030]Clean the purchased qualified raw material Achilles tendon with deionized water, cut the Achilles tendon to a suitable size with a slicer and put it in an enzymatic hydrolysis tank for later use. Add water for injection to 1000g of bovine Achilles tendon, add an appropriate amount of hydrochloric acid to adjust the pH to 4.0, add papain for enzymolysis, the concentration of papain is 100mg / L, and the temperature is 30°C for 80 hours. After the enzymolysis is completed, put the solution in the enzymolysis tank into a centrifuge and centrifuge at a speed of 10000r / min for 10min. Take the supernatant. Pour the centrifuged supernatant into a stainless steel barrel, add saturated sodium chloride solution into the stainless steel barrel, the volume ratio of the saturated sodium chloride solution to the supernatant is 1:50, and keep stirring until all the collagen is precipitated. Put the salted-out collagen into a dialysis bag and put it into a dialysis tank for dialysis for ...

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PUM

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Abstract

The invention discloses a preparation method of collagen sponge. The method comprises the following steps: carrying out enzymolysis on cattle heel tendon in protease aqueous solution at pH value of 1.0-5.0; centrifuging the enzymolysis liquid, collecting supernatant, and salting out collagen with saturated salt solution; dialyzing salted-out collagen; cross-linking the dialyzed collagen in a cross-linking agent aqueous solution; and freeze-drying cross-linked collagen gel, cross-linking the collagen in a cross-linking agent aqueous solution and freeze-drying to obtain the collagen sponge. The collagen sponge obtained by the preparation method of the collagen sponge disclosed by the invention has certain elastic and tensile toughness after being immersed in water, and has good biocompatibility and tissue repairing performance, and high protein content and is controllable in degradation.

Description

technical field [0001] The invention relates to a preparation method of a collagen sponge. Background technique [0002] Collagen is a kind of extracellular protein, which is a fibrous protein with three peptide chains twisted into a spiral shape. Collagen is the most abundant protein in the human body, accounting for more than 30% of the total protein in the body. Collagen is rich in amino acids such as glycine, proline, and hydroxyproline that the human body needs. Collagen is the most important component of the extracellular matrix. Collagen is considered to be one of the most useful biomaterials due to its superior biocompatibility and safety. The main applications of collagen as a drug delivery system are: collagen masks in ophthalmic surgery, collagen sponges in wounds and burns, pellets and tablets for protein delivery, controlled release gel formulations, controlled release materials for transdermal drug delivery, gene delivery Nanoparticles and cell culture subst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L27/24A61L27/60C12P21/06
Inventor 宋文领石凌锋
Owner 北京益而康生物工程有限公司
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