Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Enterovirus 71 (EV71) monoclonal antibody and application thereof

A monoclonal antibody and virus technology, applied in the field of virus immunity biomedicine, can solve the problems of high test cost, long detection time, difficult to popularize and use, etc., and achieve the effect of strong guiding significance, high neutralization activity and so on.

Active Publication Date: 2012-04-11
SINOVAC BIOTECH
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the test cost is high, many large-scale instruments and equipment and skilled technicians are required, the operation is complicated, the detection time is long, and it is not easy to promote and use in our country.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enterovirus 71 (EV71) monoclonal antibody and application thereof
  • Enterovirus 71 (EV71) monoclonal antibody and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The establishment of embodiment 1 anti-EV71 monoclonal antibody cell line

[0040] 1. Immunogen preparation: the immunogen is EV71 inactivated virus (the virus was preserved in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC) on May 8, 2009, and the preservation number is CGMCC No.3062) to inoculate Vero cells , obtained through culture, inactivation and purification.

[0041] 2. Animal immunization: BALB / c mice were immunized with EV71 inactivated virus. For the first time, animals were immunized with complete Freund's adjuvant mixed with EV71 emulsified according to methods known in the art; 10 days later, mice were boosted with Freund's incomplete adjuvant mixed with EV71 emulsified; one week later, serum anti-EV71 activity was measured by indirect ELISA. The mouse with the highest antibody titer was boosted intraperitoneally with EV71 purified solution.

[0042] 3. Cell fusion strain establishment: 3 ...

Embodiment 2

[0044] The preparation of embodiment 2 monoclonal antibody

[0045] BALB / c mice were immunoparaffinized intraperitoneally. Hybridoma cells were cultured, collected, centrifuged and washed once, resuspended in serum-free culture medium, cell density was adjusted, and sensitized mice were injected intraperitoneally for 7 days. About 7-12 days after the inoculation, the abdominal cavity of the mouse was obviously swollen. The ascites was collected with a syringe, centrifuged at 1500rpm for 10 minutes to collect the ascites, and the monoclonal antibody cell line was established and stored in liquid nitrogen.

Embodiment 3

[0046] The specificity determination of embodiment 3 monoclonal antibody

[0047] Use A9 monoclonal antibody purified by ammonium sulfate to coat the microtiter plate, add EV71 inactivation solution of A, B, C three genotypes, Cox A16 virus, hepatitis A virus, Japanese encephalitis virus, varicella virus, etc., and mark with HRP The rabbit anti-EV71 polyclonal antibody is a labeled antibody for antigen detection. The results showed that only EV71 virus reacted, and the monoclonal antibody had no cross-reaction with other enteroviruses and other hand-foot-mouth viruses such as Cox A16, and the specificity was good.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an enterovirus 71 (EV71) monoclonal antibody and an application thereof. The monoclonal antibody is derived from an EV71 hybrid tumor cell strain, which has a preservation number of CGMCC (China General Microbiological Culture Collection) No.5330. The monoclonal antibody and related reagents thereof have important significance in promoting the treatment and research of severe cases and death cases caused by EV71 in China, and have extremely important value in further promoting clinical research, epidemic development and passive treatment of EV71.

Description

technical field [0001] The invention relates to the field of virus immune biomedicine, in particular to an enterovirus 71 monoclonal antibody and its application. Background technique [0002] Hand, foot and mouth disease (Hand, foot and mouth diseases, HFMD) is a fecal-oral disease that is widely prevalent in the world, and it has been reported in most parts of the world. For a long time, mild hand, foot and mouth disease broke out periodically all over the world. The disease was first reported in New Zealand in 1957, the Coxsackie virus was isolated for the first time in 1958, and the name of hand, foot and mouth disease was proposed in 1959. In 1969, enterovirus 71 (EV71 virus) was isolated from stool samples of infants with central nervous system diseases in the United States, and the virus was first confirmed. The interval between the prevalence of HFMD is 2-3 years. The early pathogens were mainly Cox A16, and later EV71 and Cox A16 appeared alternately, becoming the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/20C07K16/10G01N33/577A61K39/42A61P31/14C12R1/91
Inventor 蔡芳高强王琳薛晨宝张立志尹卫东
Owner SINOVAC BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com