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Multi-component bone tissue engineering scaffold material and preparation method thereof

A technology of bone tissue engineering and scaffold materials, applied in medical science, prosthesis, etc., can solve problems such as easy aggregation and uneven dispersion of HA particles, and achieve the effect of promoting repair, good application prospects, and simple method process

Inactive Publication Date: 2012-04-11
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a multi-component bone tissue engineering scaffold material and a preparation method thereof. The bone tissue engineering scaffold material prepared by the method has a structure similar to natural bone and excellent mechanical properties, and overcomes the disadvantages of traditional scaffolds. HA particles have the disadvantages of uneven dispersion and easy agglomeration, and can play the role of each component at the same time, so it has a good application prospect in bone tissue engineering

Method used

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  • Multi-component bone tissue engineering scaffold material and preparation method thereof
  • Multi-component bone tissue engineering scaffold material and preparation method thereof

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Embodiment 1

[0026] Dissolve 1 g of pectin in 100 mL of deionized water to prepare a pectin solution with a concentration of 1% (w / v); dissolve 1 g of chitosan in 100 mL of 1% acetic acid solution to prepare a concentration of 1% ( w / v) acetic acid solution of chitosan; 4.723g calcium nitrate (Ca(NO 3 ) 2 4H 2 O) Dissolved in pectin solution, 1.653g sodium dihydrogen phosphate (NaH 2 PO 4 2H 2 O) be dissolved in the chitosan solution; the above-mentioned chitosan solution containing phosphate is added dropwise in the pectin solution containing calcium ions, wherein the ratio of calcium ions to the amount of phosphate radicals is 1.67, and the mass concentration is 5% NaOH solution was used to adjust the pH value to 9, stirred for 6 hours, and allowed to stand still for 48 hours to maintain the pH value of 9. Centrifuge, collect the solids, and rinse with deionized water until the pH value of the solution is 7, pre-freeze at -20 °C, and then freeze-dry to obtain nano-hydroxyapatite / chi...

Embodiment 2

[0029] Dissolve 1 g of pectin in 100 mL of deionized water to prepare a pectin solution with a concentration of 1% (w / v); dissolve 1 g of chitosan in 100 mL of 1% acetic acid solution to prepare a concentration of 1% ( w / v) acetic acid solution of chitosan; 2.362g calcium nitrate (Ca(NO 3 ) 2 4H 2 O) Dissolved in pectin solution, 0.827g sodium dihydrogen phosphate (NaH 2 PO 4 2H 2O) dissolved in chitosan solution; the chitosan solution containing phosphate is added dropwise to the pectin solution containing calcium ions, wherein the ratio of calcium ions to phosphate radicals is 1.67, and the mass concentration is 5 % NaOH solution was used to adjust the pH to 9, stirred for 6 hours, and allowed to stand for 48 hours to maintain the pH at 9. Centrifuge, rinse with deionized water until the pH of the solution is 7, pre-freeze at -20 °C, and freeze-dry to obtain nano-hydroxyapatite / chitosan-pectin complex (nHCP).

[0030] Dissolve 1g of chitosan in 50mL of acetic acid with...

Embodiment 3

[0032] Dissolve 1g of pectin in 100mL of deionized water to prepare a pectin solution with a concentration of 1% (w / v); dissolve 0.5g of chitosan in 100mL of 1% acetic acid solution to prepare a concentration of 0.5% (w / v) acetic acid solution of chitosan; 4.723g calcium nitrate (Ca(NO 3 ) 2 4H 2 O) Dissolved in pectin solution, 1.653g sodium dihydrogen phosphate (NaH 2 PO 4 2H 2 O) dissolved in chitosan solution; the chitosan solution containing phosphate is added dropwise to the pectin solution containing calcium ions, wherein the ratio of calcium ions to phosphate radicals is 1.67, and the mass concentration is 5 % NaOH solution was used to adjust the pH to 9, stirred for 6 hours, and allowed to stand for 48 hours to maintain the pH at 9. Centrifuge, rinse with deionized water until the pH of the solution is 7, pre-freeze at -20 °C, and freeze-dry to obtain nano-hydroxyapatite / chitosan-pectin complex (nHCP).

[0033] Dissolve 1g of chitosan in 50mL of acetic acid with...

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Abstract

The invention discloses a multi-component bone tissue engineering scaffold and a preparation method thereof. The multi-component bone tissue engineering scaffold is prepared from chitosan, gelatin, pectin and nano hydroxyapatite by weight percent. The preparation method comprises the following steps of: selecting the chitosan and the pectin to prepare a hydroxyapatite / chitosan-pectin compound by virtue of a codeposition technology, wherein the particle diameter of the hydroxyapatite is 10-100nm; and dispersing nHCP into a chitosan-gelatin solution, crosslinking with glutaric dialdehyde and then freeze-drying to obtain the multi-component bone tissue engineering scaffold. In the scaffold prepared by the preparation method disclosed by the invention, nHCP can be uniformly dispersed in the scaffold and is difficult to agglomerate; the pore diameter of the scaffold is 100-300 mu m, and the porosity of the scaffold is greater than 80%; and the scaffold disclosed by the invention can be used for promoting the adhersion and growth of skeletogenous cells, has good biocompatibility and is expected to be used as a novel bone tissue engineering biological material.

Description

technical field [0001] The invention relates to a multi-component bone tissue engineering scaffold material and a preparation method thereof, belonging to the bone repair biomaterial technology. Background technique [0002] At present, various orthopedic diseases are becoming more and more serious, and bone repair and regeneration is a common and complex clinical problem. Many people died due to lack of ideal replacement materials. The current bone graft materials mainly include autologous bone, allograft bone, and specially treated xenograft bone, but these strategies have certain limitations and their use is limited. Scaffold-based bone tissue engineering strategy is an effective way to solve bone defects. Ideal bone tissue engineering materials should have suitable biodegradability, mechanical properties and biocompatibility. A suitable microenvironment should be provided for the growth of cells and tissues. It not only provides support for the growth of cells, but m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/12A61L27/56
Inventor 姚芳莲李俊杰尹建伟刘玉玺
Owner TIANJIN UNIV
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