Production method for preparing high-purity oligosaccharides by hemicellulose enzymolysis

Abstract

The invention provides a method for preparing high-purity oligosaccharides by the enzymolysis of hemicellulose containing mannosan, and a product which is a high-effect beta-mannase BM-II produced by fermenting bacillus subtilis TQBm with the preservation No. of CCTCC No: M 211147. Konjac glucomannan, guar gum, sophora bean gum, sesbania gum and other hemicelluloses containing the mannosan are hydrolyzed into relatively small molecule materials, and then are desalted, decolored and deodorized through centrifugal separation, flocculation clarification and ion exchange techniques, large molecule materials in sugar solution are separated by the combination of an ultrafiltration technique and a molecular sieve chromatography technique, and finally the high-purity oligosaccharides is prepared through concentration and drying. The method for preparing the high-purity oligosaccharides disclosed by the invention has the advantages of simple operation, good separation effect, low cost and no chemical pollution, and industrialized production can be realized. The oligosaccharides product disclosed by the invention can obviously proliferate bifidobacteria in the human intestinal tract and otherbeneficial bacteria and obviously reduce enterobacter and other harmful bacteria, has the functions of blood sugar and blood fat reduction and immunoregulation, and is a novel raw material and assistant for gastrointestinal health, blood sugar and fat reducing, immunoregulation and disease resisting and preventing medicines.

Description

technical field [0001] The invention relates to enzyme technology and biochemical technology, more precisely, it is a preparation method and product of mannan oligosaccharides with a purity of 98% (P98) by enzymatically hydrolyzing hemicellulose containing mannan. , Immunomodulation, new raw materials and additives for anti-disease and disease-preventive drugs. Background technique [0002] Hemicellulose refers to the part of plant polysaccharides that grow symbiotically with cellulose in plant cell walls, are soluble in alkaline solutions, and are far easier to hydrolyze than cellulose when exposed to acid. There are three main types of hemicelluloses, namely xylosaccharides, polyglucomannose and polygalactosylglucomannose. The hemicellulose involved in the present invention is polyglucomannose (representative product is konjac gum) and polygalactomannose (representative product is guar gum), collectively referred to as mannan. [0003] Mannan in hemicellulose is enzymati...

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Problems solved by technology

[0004] So far, in the research on the preparation method of low-carbon chain mannose heteropolymers, either the enzyme activity is not high, the substrate concentration is low, the cost is high, and the basis for industrial production is lacking; or the addition of alkali and acid during the hydrolysis process , which brings difficulties to the post-treatment process and is not conducive to environmental protection; or the purity of the product is low, generally only 70-80%, although in the research results of Huang Daiyong et al. (2007), the purity of konjac manna oligosaccharides has reached 95%, but the degree of polymerization of sugar molecules is as high as 3 to 10 sugars. Due to the limitation of functions, it can only be used in food and chemical products, and there is still a certain gap in the application to pharmaceutical products.

In particular, the research level of galactomannan oligosaccharides is still very low, and there is still a big gap from industrialization

Therefore, the diversity of raw materials and high-purity preparation methods of mannooligosaccharides still face many technical difficulties

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