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Bacillus alcalophilus and application thereof to fermentative hydrogen production

A technology of thermophilic bacillus and application method, which is applied in the field of thermophilic bacillus and its application in fermentative hydrogen production, can solve the problems of large infrastructure and operating costs, high manufacturing requirements, long delay time, etc., and achieve obvious economical Value and development potential, improved bioredegradability, and short delay time effect

Inactive Publication Date: 2012-03-14
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has a long delay time (from the start of fermentation to hydrogen production), low purity of hydrogen, and large power consumption, which requires high equipment manufacturing and increases production costs.
In addition, ultrasonic, ozone oxidation, chlorine oxidation, thermal hydrolysis, acid or alkali treatment and other physical and chemical methods have chloroform by-products, Maillard reaction inhibits anaerobic digestion, large infrastructure and operating costs, high energy consumption, and potential safety hazards And other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 100ml of sludge from the secondary settling tank of the urban sewage plant, the TSS of the sludge is 13.26g / L, and the VSS is 7.41g / L. Place in a thermophilic bacteria hydrolysis reactor, and inoculate 10ml of bacterial solution of Bacillus thermophilic A11 strain (take A11 bacterial strain and inoculate it in a liquid medium, culture it in a 65°C water bath with shaking for 24 hours to prepare the bacterial solution of A11 bacterial strain, and the medium mainly The ingredients are: beef extract 5g, peptone 10g, NaCl 5g, water 1000ml, pH 7.0, 1.05kg / cm 2 , sterilized at 121°C for 20 minutes), the experimental temperature was set at 65°C, the ventilation rate was 0.21L / min, and the treatment was carried out for 48 hours to release the organic nutrients in the sludge and at the same time enrich the product in the sludge Hydrogen bacteria can kill hydrogen-consuming bacteria and pathogenic bacteria, and can release some heavy metals adsorbed in sludge. After pretrea...

Embodiment 2

[0030] Take 100ml of organic waste from marine farms. The TCOD of the waste is 12945mg / L, the SS is 1.74g / L, and the VSS is 0.62g / L. Get 100ml of organic waste from marine farms, inoculate 10ml of Bacillusthermophilic A11 bacterium liquid of the present invention, adjust pH=7, at 65°C, stirring rate 140r / min, process 24h, then inoculate Bacillus thermophilic A11 to treat sludge 10ml, at a temperature of Under the condition of 35°C, hydrogen is produced by fermentation, hydrogen is produced after 2 hours, no methane is produced, the content of hydrogen in the produced biogas is greater than 70%, and the maximum hydrogen production rate reaches 12.59ml / gCOD. The generated high-purity hydrogen is collected in the gas collection device and can be used after purification.

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Abstract

The invention relates to bacillus alcalophilus and an application thereof to fermentative hydrogen production. The steps of sludge and / or organic waste pretreatment and fermentative hydrogen production are adopted. The bacillus alcalophilus is characterized in that in the sludge and / or organic waste pretreatment step, bacillus alcalophilus liquid is inoculated in sludge and / or organic wastes, and bacillus alcalophilus hydrolysis pretreatment sludge and / or organic wastes are obtained after the treatment. In addition, the prepared bacillus alcalophilus hydrolysis pretreatment sludge can also be used as hydrogen production floras to be inoculated into the organic wastes for producing hydrogen through fermentation. A bacillus alcalophilus sludge pretreatment method can be effectively used for screening efficient hydrogen production floras in the sludge, inhabiting the growth of hydrogen consumption bacteria and killing pathogenic bacteria in the sludge and / or organic wastes. The sludge and / or organic wastes through all bacterial strain treatment has / have the large hydrogen generation quantity in the subsequent fermentative hydrogen production step, the purity of hydrogen gas is high, the delay time of the hydrogen production is short, and obvious economic values and development potential are realized.

Description

technical field [0001] The invention belongs to the technical field of fermentative hydrogen production, and specifically relates to a thermophilic bacillus and its application in fermentative hydrogen production, that is, the thermophilic bacillus is applied to the hydrolysis pretreatment of sludge / or organic waste, and the hydrolyzed bacteria are reused Fermentation of sludge / or organic waste to produce hydrogen; also includes a screened strain of thermophilic Bacillus A11. Background technique [0002] Due to the massive use of non-renewable resources such as coal and oil, human beings are not only facing the depletion of resources, but also pollute the environment and produce the greenhouse effect. Hydrogen energy has become the most attractive alternative energy source in the 21st century due to its clean and non-polluting, high calorific value, high thermal efficiency, wide application range, renewable and many other advantages. At present, hydrogen can be produced in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C02F11/04C12N1/20C12R1/07
Inventor 郭亮佘宗莲马启敏赵珺卢明敏纵岩
Owner OCEAN UNIV OF CHINA
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