Preparation method and application of multiflower knotweed vine polysaccharide chelate zinc
A technology of Shouwuteng and chelated zinc, applied in the field of medicine, can solve problems such as low absorption rate and loss, and achieve the effects of simple method, low absorption rate and abundant raw materials.
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Embodiment 1
[0014] Weigh 400g of dried Shouwu vine powder, add water to extract 3 times at 90°C, 3 hours each time, add 4000g (or 4000ml) of water 10 times the weight of the powder for the first time, add the weight of the powder for the second and third times 3200g (or 3200ml) of 8 times water, filter, combine the filtrates, concentrate to a solution with a relative density of 1.1, and obtain the polysaccharide extract of Polygonum multiflorum, then add an equivalent amount of 0.5mol / L zinc sulfate solution, stir evenly, and use 2mol / L sodium hydroxide to adjust the pH of the solution to 6.0, react at 80°C for 2.5 hours, dialyze with a dialysis bag with a molecular weight cut-off of 8000-14000, concentrate the dialysate to a solution with a specific gravity of 1.3, add 95% ethanol to adjust the alcohol content to 80 %, let stand overnight, centrifuge, dry the precipitate, and pulverize it to obtain polysaccharide chelated zinc from Polygonum multiflorum.
Embodiment 2
[0016] Weigh 600g of dried Shouwu vine powder, add water and extract twice at 80°C for 3 hours each time, add 5400g (or 5400ml) of water 9 times the weight of the powder for the first time, add 5400g of water 9 times the weight of the powder for the second time (or 5400ml), filter, combine the filtrates, concentrate to a solution with a relative density of 1.2, and obtain the polysaccharide extract of Polygonum multiflorum, then add an equivalent amount of 0.5mol / L zinc sulfate solution, stir evenly, and oxidize with 2mol / L hydroxide Adjust the pH of the solution to 7 with sodium, react at 90°C for 2 hours, dialyze with a dialysis bag with a molecular weight cut-off of 8000-14000, concentrate the dialysate to a solution with a specific gravity of 1.2, add 95% ethanol to adjust the alcohol content to 85%, and let stand overnight , centrifuged, dried the precipitate, and pulverized to obtain polysaccharide polysaccharide chelated zinc.
Embodiment 3
[0018] Weigh 1000g of dried Shouwu vine powder, add water to extract 4 times at 100°C, 1.5 hours each time, add 10000g (or 10000ml) of water 10 times the weight of the powder for the first time, add the weight of the powder for the second and third times 9 times the water 9000g (or 9000ml), the fourth time add 8000g (or 8000ml) of water 8 times the weight of the powder, filter, combine the filtrate, concentrate to a solution with a relative density of 1.3 to obtain the polysaccharide extract of Polygonum multiflorum, then add Amount of 0.5mol / L zinc sulfate solution, stir evenly, adjust the pH of the solution to 8 with 2mol / L sodium hydroxide, react at 70°C for 3 hours, dialyze with a dialysis bag with a molecular weight cut-off of 8000-14000, and concentrate the dialysate to For a solution with a specific gravity of 1.3, add 95% ethanol to adjust the alcohol content to 90%, let it stand overnight, centrifuge, dry the precipitate, and pulverize it to obtain polysaccharide polys...
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