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Method for extracting tea polysaccharide

An extraction method and technology of tea polysaccharides, which are applied in the field of tea polysaccharide extraction, can solve the problems of reducing bioavailability, inactivation of tea polysaccharides, and easy destruction of polysaccharide structures, so as to ensure effective wall breaking at low temperature, small activity damage, and improved resistance. The effect of oxidation activity

Inactive Publication Date: 2013-09-18
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional tea polysaccharides are extracted with cold or warm water, and polysaccharides are difficult to diffuse out of the cells; high temperature can easily inactivate tea polysaccharides; and acid and alkali methods can easily destroy the structure of polysaccharides and reduce their bioavailability

Method used

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  • Method for extracting tea polysaccharide
  • Method for extracting tea polysaccharide
  • Method for extracting tea polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1 Materials and reagents

[0036] Fresh thick old tea leaves (West Lake Longjing, provided by Hangzhou Longguan Industrial Co., Ltd.); For analytical purity.

[0037] 2 experimental equipment

[0038] UV-2000 ultraviolet spectrophotometer; DFY-400 pulverizer; SANYO ultra-low temperature refrigerator; RE-52A rotary evaporator; WVD-01 microwave vacuum drying oven.

[0039] 3 Experimental methods

[0040] 1) Pretreatment of coarse and old tea leaves

[0041] Take 1000g of fresh coarse old tea leaves and freeze at -80°C for 18 hours, freeze and pulverize them, pass through a 60-mesh sieve to obtain tea powder, reflux with absolute ethanol for 3 hours to remove the fat surrounded by extracellular lipids in the tea leaves, and then use 80% ethanol by volume fraction Soak at room temperature for 24 hours, filter to remove small molecular impurities, monosaccharides, disaccharides, oligosaccharides, glycosides, alkaloids, tea polyphenols, amino acids and alcohol-soluble pro...

Embodiment 2

[0082] The experimental operation is the same as that in Example 1, except that the thick and old tea leaves are frozen at -80°C for 12 hours at an ultra-low temperature. Other operations and conditions are the same as in Example 1, and 1.36 g of tea polysaccharides are obtained, the polysaccharide content is 56.84%, and the dissolution rate of tea polysaccharides is 4.53. %, the antioxidant capacity is 81.6%.

Embodiment 3

[0084] The experimental operation is the same as in Example 1, except that the thick and old tea leaves are frozen at -80°C for 24 hours at an ultra-low temperature, and other operations and conditions are the same as in Example 1 to obtain 1.73g of tea polysaccharide, with a polysaccharide content of 57.36%, and a tea polysaccharide dissolution rate of 5.76%. %, the antioxidant capacity is 84.7%.

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Abstract

The invention discloses a method for extracting tea polysaccharide, which comprises the following steps of: (1) performing ultralow temperature freeze drying on coarse and old tea at -80DEG C for 12 to 24 hours, grinding, and sieving to obtain tea powder; (2) performing refluxing extraction on the tea powder by using an organic solvent for 2 to 4 hours, soaking in 70 to 90 percent aqueous solution of ethanol for 24 to 36 hours, filtering, drying a filter cake, and thus obtaining the pretreated tea powder; and (3) adding water into the pretreated tea powder in the step (2), performing ultrasonic extraction at the temperature of between 0 and 40DEG C and the frequency of between 20 and 40KHz for 10 to 60 minutes to obtain a primary extracting solution, centrifuging the primary extracting solution to obtain primary supernate and primary residue, and separating and purifying the primary supernate to obtain the tea polysaccharide. The obtained product has high purity and high antioxidant activity; and process conditions are mild, the structure of the polysaccharide is hardly damaged, and the method is environment-friendly, meets the production requirement of health foods, is suitable for keeping the activity of thermosensitive polysaccharide, and is suitable for industrial production.

Description

(1) Technical field [0001] The invention relates to a method for extracting tea polysaccharides, in particular to a method for extracting tea polysaccharides from coarse and old tea leaves. (2) Background technology [0002] my country is one of the major tea producing countries. The tea tree planting area accounts for about 50% of the world's tea garden area, ranking first in the world. The total tea output exceeds 1 million tons, accounting for about 31% of the world's tea output. However, only the young leaves have been fully utilized for a long time, and a large amount of thick and old tea has been backlogged in warehouses or abandoned in tea gardens. The backlog of thick and old tea is more than 200,000 tons, which can only be used as fertilizer or fuel in the end, resulting in a huge waste of resources. waste. Therefore, there is an urgent need to develop the processing industry of coarse and old tea, to find a breakthrough in the deep processing of coarse and old tea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 张拥军陈萍朱丽云金晖李佳
Owner CHINA JILIANG UNIV
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