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Alternaria alternata DB2 strain and preparation and application thereof

A technology for Alternaria spp. and strains, applied to Alternaria spp. DB2 strain and the field of preparation and application thereof, can solve the problems of high energy consumption of chemical treatment process, unrecyclable waste water, large equipment loss and the like, and is suitable for large-scale industrial production. , Short degumming time, good quality effect

Inactive Publication Date: 2013-01-16
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical treatment process consumes a lot of energy, equipment loss is large, and the discharged wastewater cannot be recycled, causing serious pollution problems

Method used

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  • Alternaria alternata DB2 strain and preparation and application thereof
  • Alternaria alternata DB2 strain and preparation and application thereof
  • Alternaria alternata DB2 strain and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Screening and Obtaining of Flax Degumming Strains

[0059] (1) Sampling rotting seaweed from the Zhoushan sea area of ​​the East China Sea, first put the seaweed and the enrichment medium at a ratio of 1g: 20ml in the enrichment medium and culture it at room temperature for 30 days, then take 0.1ml of the enrichment medium and apply it to the separation medium Culture medium, static culture at 30°C for 1 to 4 days, to obtain the dominant strain of wild-type flax biotreatment;

[0060] Among them, the formula of the enrichment medium is: 5 g of flax powder, 200 ml of tap water, and the pH is natural; the formula of the separation medium is: 5 g of flax powder, (NH 4 ) 2 SO 4 5g, K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01, 20g agar, 1000mL water, adjust the pH to 7-7.2 with NaOH;

[0061] (2) Inoculate the bacterial strain obtained in the step (1) into flax lignin nutrient medium, and culture at 28° C. for 72 hours. Flax lignin nutrient medium, its compo...

Embodiment 2

[0064] Preparation of fermentation broth:

[0065] (1) Alternaria DB2 strains were stored in potato dextrose agar medium, cultured at 30°C and 220rpm for 48 hours, and added freezing buffer; freezing buffer consisted of potassium dihydrogen phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, sodium citrate 0.0588g, 0.02645g of magnesium sulfate heptahydrate, 10ml of glycerin, add water to make up to 100ml;

[0066] (2) In the sterilized and cooled 50mL potato dextrose medium, insert the bacteria ring obtained in step (1), and shake the flask for 48 hours at a rotation speed of 220r / min and a temperature of 30°C;

[0067] (3) Inoculate the culture medium obtained in step (2) into 5 L of flax lignin fermentation medium, and shake the flask for 48 hours at a rotation speed of 220 r / min and a temperature of 30° C. to obtain a fermentation broth; wherein, the formulation of the flax fermentation medium is: : Flax raw hemp lignin 5g, KCl 0.05g, dipotassium hydrogen phosphate...

Embodiment 3

[0069] The flax degumming process is as follows:

[0070] Put 5g of the original stem of flax into a 250ml conical flask, put the original flax stem and the fermented bacterium liquid of Example 2 into the conical flask according to the bath ratio of 1:20, the liquid volume is 100ml, at 40°C, in a shaker at 200rpm Respectively degumming for 1 day; when the degumming time is up, remove the degumming solution and stop the biological treatment. The biologically treated raw flax stems are inactivated, and the process is as follows: the biologically treated flax raw stems are mixed with the degumming solution at a ratio of 1:10, and treated at 70°C for 3 hours, wherein the peroxide The dosage is 3% of the original flax stem, the dosage of urea is 0.05% of the original flax stem, the dosage of sodium tripolyphosphate is 2% of the original flax stem, and the dosage of disodium edetate is 0.1% of the original flax stem . After degumming, 2130 flax fibers for textile can be produced....

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Abstract

The invention relates to an Alternaria alternata DB2 strain and preparation and application thereof. The preparation method comprises the following steps of: (1) putting rotten seaweed into an enrichment culture medium, standing and culturing at room temperature, coating the enrichment culture medium in an isolation culture medium, and standing and culturing for 1 to 4 days at the temperature of 30 DEG C to obtain wild flax biological treatment strains; (2) inoculating the strains obtained in step (1) into a flax lignin nutrient culture medium, and culturing for 72h at the temperature of 28 DEG C; and (3) selecting a strain which has the capacity of degrading lignin from the flax lignin nutrient culture medium to obtain the Alternaria alternata DB2 strain. The strain can be applied to a process for preparing textile flax, hemp, jute or kenaf fiber by degumming peroxide. The growth cycle of the strain is short, the strain cannot be polluted easily, has strong contamination resistance and good heat resistance, and does not pollute the environment, the treatment cost is low, reaction conditions are mild, and the quality of treated fiber is high; and the process is simple and is suitable for large-scale industrialized production.

Description

technical field [0001] The invention belongs to the field of Alternaria strains and their preparation and application, in particular to an Alternaria DB2 strain and its preparation and application. Background technique [0002] Hemp is one of the raw materials for textiles, and its varieties mainly include flax, ramie, hemp, jute, kenaf, apocynum, sisal, abaca, nettle and velvetle. Hemp fiber is a general term for fibers obtained from various hemp plants. It has the incomparable advantages of other natural fibers such as moisture absorption, breathability, heat dissipation and antibacterial. As one of the countries with the richest hemp resources in the world, my country produces hemp fiber and its products not only to meet the needs of the domestic market, but also exported to the United States, Western Europe and Southeast Asian countries as bulk commodities, with an average annual foreign exchange earning of nearly 10 billion U.S. dollars. Driven by the national energy-s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02D01C1/00C12R1/645
Inventor 丁若垚刘国亮董政娥郑磊郁崇文张兴群
Owner DONGHUA UNIV
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