Lactobacillus reuteri strain and application thereof

A technology of Lactobacillus reuteri and microbial strains, applied in the direction of application, bacteria, microorganisms, etc., can solve the problems of limited application, unsystematic research on application, and small number of reports, so as to promote animal growth and enhance non-specific immunity function, the effect of improving conversion efficiency

Active Publication Date: 2012-01-04
北京龙科方舟生物工程技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Lactobacillus reuteri is mainly used in human yogurt food in China, and the number of reports is small, and there are even fewer reports in the field of animals. The main reason may be that there are no applications of the bacteria from isolation and identification to product preparation and animal production. Systematic research, thus limiting the application of this strain in the field of animals

Method used

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  • Lactobacillus reuteri strain and application thereof
  • Lactobacillus reuteri strain and application thereof
  • Lactobacillus reuteri strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1, isolation and identification of Lactobacillus reuteri (Lactobacillus reuteri) RI021

[0074] The Lactobacillus reuteri of the present invention is obtained by extracting, separating, screening and purifying from pig gastrointestinal mucosa.

[0075] (1) Isolation and purification of Lactobacillus reuteri

[0076] 1.1 Test material

[0077] Take healthy piglet gastrointestinal mucosa 5cm 2 (stomach, duodenum, jejunum, ileum, cecum, and colon), in a sterile cup, rinse the chyme on the mucosa with sterile saline, immerse the mucosa in a beaker containing 15 mL of HEPES buffer, and use forceps After shaking the mucosa back and forth for 5 minutes, the supernatant was obtained.

[0078] 1.2 Isolation and cultivation of strains

[0079] Use a sterile syringe to draw 0.2 mL of the supernatant into the Hungates roller tube filled with Rogosa SL agar medium, and gently roll the supernatant by hand to evenly distribute the supernatant on the surface of the agar ...

Embodiment 2

[0146] Example 2. Research on the biological characteristics of Lactobacillus acidophilus RI021 CGMCC No.4650

[0147] 1.1 Growth curve

[0148] Fill 300mL MRS broth medium in a 500mL Erlenmeyer flask. Inoculate the culture of Lactobacillus reuteri according to 1% inoculation amount, take samples every 1 hour in the first 24 hours, and take samples at 28, 32, 36, 40, 44, 48 hours after 24 hours, take 1 mL of culture solution for gradient dilution, and then in 10 -2 ~10 -6 Dilution Take 0.3mL dilution and spread evenly on MRS agar medium (pH5.2), make 3 parallel samples for each gradient, place the plate at 37°C, 5% CO 2 In the incubator, cultivate for 24 hours, take the dilution of 50-150 colonies in the plate for calculation, make 3 parallel samples for each gradient, and express the results as the average value. Bacterial concentration per milliliter is expressed as a logarithmic value.

[0149] 1.2 Acid-resistant survival rate

[0150] MRS broth medium was adjusted to ...

Embodiment 3

[0177] Embodiment 3, the preparation of Lactobacillus reuteri (Lactobacillus reuteri) RI021 CGMCC No.4650 preparation

[0178] 1. Inoculate Lactobacillus reuteri in glucose nutrient broth, adjust the pH to 6.7 with acetic acid, place it in an ordinary incubator at 37°C for 18-24 hours, and then dilute it with sterile water to obtain a concentration of (10 7 -10 10 ) cfu / g of the strain solution.

[0179] By weight, mix 1 part of the strain solution containing Lactobacillus reuteri CGMCC No.4650, 1 part of rice husk powder, 1 part of skimmed milk powder, 1 part of maltodextrin, and 0.5 part of bran, dry and pulverize, that is A Lactobacillus reuteri preparation A consisting of Lactobacillus reuteri and a carrier Lactobacillus reuteri preparation was obtained. In the Lactobacillus reuteri preparation A, the proportion of the two is 8 grams of carrier: 10 10 cfu Lactobacillus reuteri, the carrier is composed of rice husk powder, skimmed milk powder, maltodextrin and bran, and ...

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Abstract

The invention discloses a lactobacillus reuteri strain and application thereof. The lactobacillus reuteri strain is lactobacillus reuteri RI021 preserved in China General Microbiological Culture Collection Center on March 11, 2011, and the accession serial number in the collection center is CGMCC (China General Microbiological Culture Collection Center) No. 4650. The lactobacillus reuteri strain and a lactobacillus reuteri preparation thereof are mainly used as feed additives for animals and can be used for regulating the microeubiosis in animal intestines, thus achieving the effect of enhancing the non-specific immune function so as to prevent diseases, simultaneously providing nutritional factors, promoting the digestion and absorption of nutrients, promoting the growth of the animals and improving the feed conversion rate.

Description

technical field [0001] The invention relates to a strain of Lactobacillus reuteri and its application. Background technique [0002] While the addition of antibiotics in feed has made a significant contribution to the development of intensive animal husbandry, its side effects such as endogenous infection and superinfection, the emergence of drug resistance, the destruction of normal intestinal flora and the The residues of antibiotics pose a serious threat to the food safety of aquaculture, animals, and humans. Therefore, research and development of new green and safe additives that replace antibiotics is an important direction for feed development. [0003] Lactobacillus reuteri (Lactobacillus reuteri) is an obligate heteromorphic lactic acid fermenting bacterium that inhabits the intestinal tract of humans and animals and has the ability to adhere to the intestinal epidermis. It is also one of the important populations of lactic acid bacteria. The U.S. Food and Drug Admi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/225A23K10/18A23K10/20A23K10/30
CPCY02P60/87
Inventor 谯仕彦宋青龙宋维平王洪彬
Owner 北京龙科方舟生物工程技术有限公司
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