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A light-colored high-molecular-weight ganoderma polysaccharide and its preparation method

A technology of high molecular weight and Ganoderma lucidum polysaccharide, which is applied in the direction of antineoplastic drugs, drug combinations, allergic diseases, etc., can solve the problems of unfavorable large-scale production of product quality, difficult to control product quality, and harmful organic solvent residues, etc., to achieve enhanced anti-tumor The effect of high capacity, uniform distribution and high content of active ingredients

Active Publication Date: 2011-12-28
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are more and more health care products based on ganoderma lucidum polysaccharides on the market, but due to the complex polysaccharides contained in ganoderma lucidum, the rough extraction method, and the high cost of intensive processing, the current health care products using ganoderma lucidum as raw materials are not purified. The active ingredients of Ganoderma lucidum are made from the latest active ingredients, which makes it difficult to control the quality of the product and the product effect is unstable
[0004] The crude product of Ganoderma lucidum obtained by conventional water extraction contains pigments, proteins and small molecular impurities, which makes the polysaccharide product darker in color and low in polysaccharide content. The existing decolorization and deproteinization technologies are relatively complicated and require activated carbon, hydrogen peroxide or even chloroform , n-butanol and other organic solvents will cause the loss of polysaccharides, and even cause the residue of harmful organic solvents, which is not conducive to large-scale production and improvement of product quality. The polysaccharide content of the obtained product is only about 50%, and the purity is not high

Method used

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  • A light-colored high-molecular-weight ganoderma polysaccharide and its preparation method
  • A light-colored high-molecular-weight ganoderma polysaccharide and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Extraction of Ganoderma lucidum crude polysaccharides: using red lucidum fruiting body (Ganoderma lucidum strain with strain No. 2442 preserved in the strain preservation center of Edible Fungi Institute of Shanghai Academy of Agricultural Sciences, obtained through artificial cultivation) as raw material, crushed into coarse grains , weigh 2kg, add 20L water, soak at room temperature for 30min, heat to boiling, keep boiling slightly for 90min, and filter. Repeat the above steps for the filter residue, extract twice more, and combine the filtrates.

[0022] 2. Concentration of ganoderma lucidum crude extract: the filtrate is coarsely filtered with a 120-mesh filter screen (purchased from Shanghai Xinhua Wire Mesh Store), and then filtered with a 100-micron membrane (purchased from Shanghai Fleet Company), and the filtrate is concentrated under reduced pressure to 3L.

[0023] 3. Ethanol precipitation and washing: slowly add absolute ethanol to the concentrated solut...

Embodiment 2

[0028] 1. Extraction of Ganoderma lucidum crude polysaccharides: use red lucidum fruiting body (the bacterial strain No. Ganoderma lucidum 2442 preserved in the strain preservation center of the Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, obtained through artificial cultivation) as raw material, crushed into coarse grains , weigh 1.5kg, add 20L of water, soak at room temperature for 30min, heat to boiling, keep boiling slightly for 60min, and filter. Repeat the above steps for the filter residue, extract once more, and combine the filtrates.

[0029] 2. Concentration of ganoderma lucidum crude extract: the filtrate is coarsely filtered with a 120-mesh filter screen (purchased from Shanghai Xinhua Wire Mesh Store), and then filtered with a 100-micron membrane (purchased from Shanghai Fleet Company), and the filtrate is concentrated under reduced pressure to 2L.

[0030] 3. Ethanol precipitation and washing: Slowly add absolute ethanol to the concentra...

Embodiment 3

[0035] Determination of the activity of Ganoderma lucidum polysaccharide to stimulate macrophages to release NO in vitro:

[0036] 1. Sample preparation: Accurately weigh the Ganoderma lucidum polysaccharide samples prepared in Example 1 and Example 2 into a sterilized eppendorf tube, and prepare a sample solution with a concentration of 5 mg / mL with sterile PBS. After fully dissolving, centrifuge at 15000r / min for 30min, transfer the supernatant to a new sterile eppendorf tube under aseptic conditions, and dilute the sample to 2mg / ml and 0.5mg / ml for use.

[0037] 2. Cell culture: take the RAW264.7 macrophage cell line in the logarithmic growth phase (purchased from the Institute of Cells, Chinese Academy of Sciences), and use DMEM complete medium (purchased from Gibco) at 37 ° C, containing 5% CO 2 The cells were subcultured under conditions, digested with 0.05% trypsin or 5% EDTA solution, and the suspension was centrifuged at 1000rpm / min for 3min to collect the cells and c...

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Abstract

The invention provides a ganoderma lucidum polysaccharide with a light color and high molecular weight, and a preparation method thereof. The ganoderma lucidum polysaccharide with a light color and high molecular weight is prepared by a method comprising the steps of extraction of crude polysaccharide of ganoderma lucidum, condensation of crude polysaccharide of ganoderma lucidum, ethanol precipitation and rinsing, and drying. The ganoderma lucidum polysaccharide prepared in the invention has a stable quality, contains high content active components and can substantially enhance phagocytosis and killing capability of macrophage, thereby improving antineoplastic capability of organism.

Description

technical field [0001] The invention relates to the field of extracting medicinal fungi, in particular to a light-colored high-molecular-weight ganoderma polysaccharide and a preparation method thereof. Background technique [0002] Ganoderma lucidum is a traditional medicinal fungus that has been used for a long time in my country. It includes Ganoderma lucidum and Zizhi. For medicinal purposes, Ganoderma lucidum is more commonly used. A large number of studies have shown that Ganoderma lucidum polysaccharide is one of its main active ingredients, which has pharmacological effects such as immune regulation, anti-tumor, anti-virus, anti-oxidation, hypoglycemic and liver protection. [0003] At present, there are more and more health care products based on Ganoderma lucidum polysaccharides in the market, but due to the complex polysaccharides contained in Ganoderma lucidum, the rough extraction method, and the high cost of intensive processing, the current health care product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/04A61P35/00
Inventor 刘艳芳张劲松唐庆九杨焱唐传红周帅吴迪冯娜贾薇汪雯翰
Owner SHANGHAI ACAD OF AGRI SCI
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