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A kind of detection method of selenomethionine in selenium-enriched yeast

A technology for selenium-enriched yeast and selenomethionine, which is applied in the field of high performance liquid chromatography for determining selenomethionine in selenium-enriched yeast, can solve the problem of increasing the difficulty of extraction and detection of selenomethionine, being difficult to adapt to laboratory testing requirements, and requiring strict equipment and equipment conditions. It is easy to popularize, reduce the cost of measurement, and achieve the effect of high sensitivity.

Active Publication Date: 2011-12-21
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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Problems solved by technology

[0004] Due to the complex matrix of selenium-enriched yeast and the easy oxidation of selenomethionine, the difficulty of extraction and detection of selenomethionine is increased.
At present, HPLC-ICP-MS method and HPLC-ESI-MS / HPLC-ESI-MS / MS method are widely used in the research to analyze the speciation of selenium in selenium-enriched yeast. The requirements for equipment and equipment are strict, and the experimental cost is expensive, so it is difficult to adapt. Laboratory routine testing requirements, the method is not easy to popularize

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  • A kind of detection method of selenomethionine in selenium-enriched yeast
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  • A kind of detection method of selenomethionine in selenium-enriched yeast

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Embodiment 1

[0051] Determination of selenomethionine in embodiment 1 selenium-enriched yeast

[0052] Sample 1 is a selenium-enriched yeast sample provided by a domestic yeast production company. The cell wall of the selenium-enriched yeast was destroyed by liquid nitrogen freezing and then mortar grinding. Weighed 0.1g of selenium-enriched yeast powder into a glass bottle and added 5mL 0.1% DTT (w / v) 0.05mol / L phosphate buffer (pH7.4), 40mg pronase, reacted on a shaker at 37°C for 24h, after the enzymolysis solution was cooled to room temperature, add 0.1mL to the enzymolysis solution 1.5g / mL potassium ferrocyanide, 0.1mL 3g / mL zinc acetate, mix well and centrifuge at 3000rpm for 10min.

[0053] Take 200 μL of the supernatant obtained after centrifugation of the enzymatic hydrolysis solution, add 1.8 mL of sodium carbonate buffer solution, 1 mL of 6000 mg / L dansyl chloride derivative solution, derivatize in a water bath at 60 ° C for 2 hours, add 0.1 mL of 20 mg / mL methylamine hydrochlor...

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Abstract

The invention discloses a method for detecting selenomethionine (SeMet) in selenium-enriched yeast and belongs to the technical field of food analysis. The method comprises the following main steps of: (1) performing enzymolysis on combined SeMet in the selenium-enriched yeast; (2) derivating the SeMet through dansyl chloride serving as a derivating agent; and (3) separating and measuring components of derivating liquid by a high performance liquid chromatography-fluorescence detection (HPLC-FLD) method, and calculating content of the SeMet in a sample by an external standard method. Enzymolysis conditions and parameters in the derivatization process are optimized, the technical problem of extracting and derivating the SeMet in the selenium-enriched yeast is solved, the HPLC-FLD method for detecting the SeMet in the selenium-enriched yeast is established, and an accurate, easy and economic effective method is provided for quality evaluation of the selenium-enriched yeast.

Description

technical field [0001] The invention belongs to the field of food analysis and relates to a high-performance liquid chromatography (HPLC) method for determining selenomethionine in selenium-enriched yeast. Background technique [0002] Functional yeast refers to the selection of suitable yeast strains. By controlling their growth environment, the yeast can enrich certain types of nutrients in the medium during the growth process and convert them into special active substances needed by certain types of people through the metabolism of yeast. At present, the most mature functional yeast product in China is selenium-enriched yeast, which uses Saccharomyces cerevisiae, and adds inorganic selenium preparations such as selenite to the medium during production. After the yeast obtains inorganic selenium, it converts it into Organic selenium mainly exists in the form of selenomethionine (organic selenium refers to selenium combined with or participating in the synthesis of biologic...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 熊正河钟其顶高洪波韦淑毅
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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