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Preparation method and application of a drug-loaded magnetic composite nanomaterial

A magnetic composite nanometer and magnetic nanoparticle technology, which is applied in the direction of medical formulas, unknown raw materials, medical preparations with non-active ingredients, etc., can solve problems such as the inability of drugs to concentrate tumor cells, failure of tumor treatment, and large toxic and side effects. Overcome multi-drug resistance, improve toxic and side effects, and improve curative effect

Inactive Publication Date: 2011-12-14
卢世璧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Tumor is a major disease that threatens human health. Chemotherapy is still one of the main methods to treat tumors. The problem with chemotherapy is that it has high toxicity and side effects, because drugs not only kill tumor cells, but also kill some normal cells, that is, drugs cannot be concentrated. Accumulated in tumor cells, or the targeting is not good; second, after the application of various chemotherapy drugs, multidrug resistance (multidrug resistance MDR) will occur, that is, tumor cells will cross multiple anticancer drugs with different structures and functions. Drug resistance, which leads to the failure of tumor therapy
[0004] In order to overcome these two major problems in tumor chemotherapy, the compounding of nanomaterials and chemotherapeutic drugs can solve these problems. Therefore, it is very necessary to explore a kind of nanomaterials with multiple functions, so as to overcome the multidrug resistance and targeting problems of current tumors to some chemotherapy drugs. At present, the difficulties and hot issues that urgently need to be solved at home and abroad, and magnetic nanomaterials have greater advantages in overcoming these problems

Method used

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  • Preparation method and application of a drug-loaded magnetic composite nanomaterial
  • Preparation method and application of a drug-loaded magnetic composite nanomaterial
  • Preparation method and application of a drug-loaded magnetic composite nanomaterial

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Embodiment 1

[0041] 1. The phacoemulsification method is used to prepare a multifunctional magnetic composite nanomaterial integrating magnetism, sustained release and treatment.

[0042] ① Synthesis of Fe by co-precipitation method 3 o 4 Magnetic nanoparticles, and then coated with oleic acid, the particle size is 8nm;

[0043] ②Extract 0.1mg / ml doxorubicin with triethylamine, and the molar ratio of doxorubicin to triethylamine is 1:3;

[0044] 3. prepare the dichloromethane solution of polylactic acid / glycolic acid copolymer (PLGA), the PLGA concentration is 5mg / ml;

[0045] ④ The Fe obtained in step ① 3 o 4 Add the magnetic material to the PLGA dichloromethane solution prepared in step 3, then add the mixed solution of adriamycin extracted by step 2 triethylamine, mix well, and make the Fe 3 o 4 The final concentration of magnetic nanoparticles is 5 mg / ml, and the final concentration of doxorubicin is 0.05 mg / ml;

[0046] ⑤ Add 2ml of the mixed solution obtained in step ④ dropwis...

Embodiment 2

[0052] 1. The phacoemulsification method is used to prepare a multifunctional magnetic composite nanomaterial integrating magnetism, sustained release and treatment.

[0053] ① Synthesis of Fe by co-precipitation method 3 o 4 Magnetic nanoparticles, and then coated with oleic acid, the particle size is 8nm;

[0054] ②Extract 0.5 mg / ml doxorubicin (DOX) with triethylamine, and the molar ratio of doxorubicin to triethylamine is 1:3;

[0055] 3. prepare the dichloromethane solution of polylactic acid / glycolic acid copolymer (PLGA), the PLGA concentration is 30mg / ml;

[0056] ④ The Fe obtained in step ① 3 o 4 Add the magnetic material to the PLGA dichloromethane solution prepared in step 3, then add the mixed solution of adriamycin extracted by step 2 triethylamine, mix well, and make the Fe 3 o 4 The final concentration of magnetic nanoparticles is 10 mg / ml, and the final concentration of doxorubicin is 0.25 mg / ml;

[0057] ⑤ Add 2ml of the mixed solution obtained in step ...

Embodiment 3

[0064] 1. The phacoemulsification method is used to prepare a multifunctional magnetic composite nanomaterial integrating magnetism, sustained release and treatment.

[0065] ① Synthesis of Fe by co-precipitation method 3 o 4 Magnetic nanoparticles, and then coated with oleic acid, the particle size is 8nm;

[0066] ② Extract 1mg / ml doxorubicin with triethylamine, the molar ratio of doxorubicin to triethylamine is 1:3;

[0067] 3. prepare the dichloromethane solution of polylactic acid / glycolic acid copolymer (PLGA), the PLGA concentration is 100mg / ml;

[0068] ④ The Fe obtained in step ① 3 o 4 Add the magnetic material to the PLGA dichloromethane solution prepared in step 3, then add the mixed solution of adriamycin extracted by step 2 triethylamine, mix well, and make the Fe 3 o 4 The final concentration of magnetic nanoparticles is 5 mg / ml, and the final concentration of doxorubicin is 0.5 mg / ml;

[0069] ⑤ Add 2ml of the mixed solution obtained in step ④ dropwise to...

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Abstract

The invention discloses a preparation method and application of a drug-loaded magnetic composite nanometer material. The fat-soluble solution (or emulsion) of the surface lipophilic magnetic nanoparticles and the drug is added to the dichloromethane solution of polylactic acid / glycolic acid copolymer (PLGA), and the three are mixed with each other to form an oil phase system. The oil phase system is added dropwise to the aqueous solution in which the surfactant Pluronic F127 is dissolved to form an oil-in-water system, and then the drug-loaded magnetic composite nanomaterial is obtained under the action of ultrasound. The drug-loaded magnetic composite nanomaterial can concentrate the drug in the tumor area under the action of an in vitro magnetic field to achieve targeting; the coating of PLGA solves the problem of mutual self-aggregation of magnetic particles, and the slow release control of the drug solves the problem It has great toxicity and side effects in the body and can be quickly taken up by tumor cells, leading to apoptosis of tumor cells and reversing the multidrug resistance of tumor cells.

Description

technical field [0001] The invention belongs to the technical field of controllable preparation and application of nanometer materials, and in particular relates to the technology of preparing multifunctional composite magnetic nanomaterials by ultrasonic emulsification and the application of the composite magnetic nanometer materials. Background technique [0002] Due to their strong magnetism and low cytotoxicity, magnetic nanomaterials have great application potential in biomedicine, such as purification of antibodies and fusion proteins, delivery of drugs or genes, immunoassays, and magnetic resonance imaging. However, there are serious defects in the application of pure magnetic nanomaterials in biomedicine. Therefore, in recent years, the multifunctionalization of magnetic nanomaterials has become a research hotspot due to its novel ideas and wide applications. Cytotoxicity and therapeutic equal advantages in one. Inorganic materials have advantages in controllability...

Claims

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Application Information

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IPC IPC(8): A61K47/34A61K47/02A61K31/704A61K35/00
Inventor 袁玫卢世璧唐芳琼黄兴禄贾艳辉郭全义陈东眭翔许文静
Owner 卢世璧
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