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Preparation method of chicken infectious bursal disease specific transfer factor

A technology for bursal disease and transfer factor, which is applied in the field of preparation of specific transfer factor for infectious bursal disease in chickens, can solve the problems of unsatisfactory, single drug efficacy and the like, achieves rapid action and eliminates immune stress response , the effect of reducing infection rate and mortality

Inactive Publication Date: 2011-11-30
骆延波
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  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the above problems of single and unsatisfactory drug effects for the treatment of chicken infectious bursal disease, the invention provides a preparation of chicken infectious bursal disease specific transfer factor (hereinafter referred to as chicken specific transfer factor) Method, prepared chicken-specific transfer factor combined with egg yolk antibody to improve clinical efficacy

Method used

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  • Preparation method of chicken infectious bursal disease specific transfer factor
  • Preparation method of chicken infectious bursal disease specific transfer factor
  • Preparation method of chicken infectious bursal disease specific transfer factor

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Embodiment 1

[0020] (1) Each healthy poultry is immunized with 10 doses of infectious bursal disease live vaccine B87 strain, and the virus content of each dose is not less than 1000ELD 50 7 days later, intramuscular injection of 20 doses of infectious bursal disease live vaccine B38 venomous strain was used to boost the immunization, and the virus content of each dose was not less than 2000ELD 50 At the same time, 0.5 mL of inactivated oil emulsion vaccine of infectious bursal disease was used to immunize subcutaneously, and the virus content was 10 7.2 TCID 50 / 0.1mL, 7 days later, inject the same dose of IBD live vaccine B38 spicy strain to boost immunization once, measure the antibody level after 10 days, and detect the antibody level of IBD in the blood by agar gel diffusion test reach 2 4 For the above, fresh spleen, kidney or bursa of Fabricius were taken from the animals, the tissue was crushed and homogenized, and then frozen and thawed repeatedly at minus 20°C-30°C for more tha...

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Abstract

The invention relates to a preparation method for a chicken infectious bursal disease specific transfer factor in the technical field of immunomodulators. The method comprises the following steps of: immunizing every healthy domestic fowl by using infectious bursal disease live vaccine B87 strain, B38 strain and infectious bursal disease oil emulsion inactivated vaccine; after antibody level reaches over 24, killing an animal and taking fresh spleen, kidney or bursa of fabricius; crushing tissues, homogenating and repeatedly freeze-thawing for over 8 times; centrifuging to take supernatant; filtering to obtain a component of which the relative molecular mass is less than 5,000 Daltons; and adding a stabilizer EDTA-Na2 (Ethylenediamine Tetraacetic Acid disodium salt) and a composite thermal protectant and disinfecting, wherein the composite thermal protectant is obtained by mixing mannitol, lactose, chitosan, glycerol, glycine and sorbitol according to mass ratio of 2:3:2:3:1:2. The transfer factor has the advantages of high activity, quick response, small dose and no residues compared with a chemical drug product and the like and quick response, convenience in use and transportation, small molecular substance and various application methods compared with a traditional Chinese medicine product and the like.

Description

technical field [0001] The invention relates to the technical field of immunomodulators, in particular to a method for preparing chicken infectious bursal disease-specific transfer factor. Background technique [0002] Infectious bursal disease (IBD) is an acute, highly contagious, immunosuppressive disease mainly affecting chicks caused by infectious bursal disease virus (IBDV). It is characterized by inflammation, necrosis, atrophy, and severe damage of lymphocytes in the bursa of Fabricius. Thereby causing immune dysfunction in chickens and interfering with the immune effects of various vaccines. The morbidity rate is high, almost 100%, and the mortality rate is low, generally 5% to 15%. It is one of the most important diseases in the poultry industry. A large number of chickens suddenly become ill, and 60% to 70% of the chickens can be affected within 2 to 3 days. The death peaks 3 to 4 days after the onset, and the death stops after 7 to 8 days. At the beginning of t...

Claims

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Application Information

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IPC IPC(8): A61K35/55A61K39/395A61K35/28A61K35/23A61P31/14A61P37/04A61K35/57
CPCY02A40/70
Inventor 骆延波徐怀英
Owner 骆延波
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