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Micro-fluidic composite chip with symmetric micro-channel structure and integrated non-contact conductivity detection

A conductivity detection and composite chip technology, which is used in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve the problems that the separation conditions cannot be kept consistent, the separation range is small, and cannot be carried out at the same time, so as to achieve rapid electrophoretic separation analysis. , the effect of improving detection sensitivity and improving the degree of integration

Inactive Publication Date: 2013-10-30
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this electrophoresis chip are: the range of isolates is small, when doing blank and sample and other control experiments, they cannot be carried out at the same time, the separation conditions cannot be kept consistent, and the reproducibility is not high
The main disadvantage of this electrophoresis chip is: although there are shielding measures, which can reduce part of the interference and can detect low-concentration substances, when it is used as a complex sample with many interference components, the reproducibility is still low.

Method used

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  • Micro-fluidic composite chip with symmetric micro-channel structure and integrated non-contact conductivity detection
  • Micro-fluidic composite chip with symmetric micro-channel structure and integrated non-contact conductivity detection
  • Micro-fluidic composite chip with symmetric micro-channel structure and integrated non-contact conductivity detection

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Effect test

Embodiment 1

[0023] like Figure 1~4 As shown, a microfluidic composite chip with a symmetrical micropipe structure integrated with non-contact conductivity detection is mainly composed of a glass substrate, a polydimethylsiloxane (PDMS) cover sheet, and a micro control circuit board. The feature is: the microfluidic composite electrophoresis chip is made of a polydimethylsiloxane cover sheet engraved with a symmetrical microchannel structure and a glass substrate deposited with a symmetrical microelectrode at room temperature. The circuit board is connected for conductance detection, and the signals in the two parallel micro-pipes are differentiated, and the separation results are compared under the same conditions.

[0024] The glass substrate has a length of 40000 μm, a width of 18000 μm and a thickness of 600 μm. On the glass substrate, aluminum is sputter-deposited on the glass substrate to form a micro-electrode of a micro-plane thin layer by adopting conventional micro-electro-mech...

Embodiment 2

[0029] A microfluidic composite chip with a symmetrical micropipe structure integrated with non-contact conductance detection, as in Example 1, wherein: the glass substrate has a length of 42000 μm, a width of 19000 μm, and a thickness of 800 μm, and gold is deposited on the glass substrate by sputtering A microelectrode with a microplane thin layer is formed on the chip, and the length of the electrophoretic separation driving microelectrode e, e′ is 4000 μm, the width is 1500 μm, and the thickness is 30 μm. The length of each microelectrode f and g of the online conductance detection microelectrode is 6500 μm, and the width is 650 μm, 30 μm in thickness, 45 μm in distance between two microelectrodes, and 0.4 μm in thickness of the silicon dioxide insulating layer deposited on the surface of conductance detection microelectrodes f and g. The length of the polydimethylsiloxane cover slip is 38000 μm, the width is 16000 μm, and the thickness is 1200 μm. The diameter of each rese...

Embodiment 3

[0031] A microfluidic composite chip with a symmetrical micropipe structure integrated with non-contact conductance detection, as in Example 1, wherein: the glass substrate has a length of 45000 μm, a width of 20000 μm, and a thickness of 1000 μm, and titanium-tungsten alloy is sputter-deposited on A microelectrode with a microplane thin layer is formed on a glass substrate. The length of the electrophoretic separation-driven microelectrode e, e' is 5000 μm, the width is 2000 μm, and the thickness is 50 μm. The width is 700 μm, the thickness is 50 μm, the distance between two microelectrodes is 50 μm, and the thickness of the silicon nitride insulating layer deposited on the surface of conductance detection microelectrodes f and g is 0.5 μm. The length of the polydimethylsiloxane cover sheet is 40000 μm, the width is 18000 μm, and the thickness is 1500 μm. The diameter of each reservoir is 3000 μm and the depth is 1500 μm. The distance between the centers of the two sample pool...

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Abstract

A micro-fluidic composite chip with a symmetric micro-channel structure and integrated non-contact conductivity detection relates to a structure of a micro-fluidic composite electrophoresis chip. The invention mainly comprises a glass substrate, a polydimethylsiloxane coverslip, and a micro control circuit board. The invention is characterized in that: the micro-fluidic composite electrophoresis chip comprises a polydimethylsiloxane coverslip etched with a symmetric micro-channel structure, and a glass substrate deposited with symmetric microelectrodes; conductivity detection is performed by the connection of a chip interface and the micro control circuit board; signals in two parallel micro channels are processed by differencing, and the differential results are compared under the same condition. The invention has the characteristics of convenient deduction of background and interference signals, high sensitivity, simple operations, good stability, quick analysis efficiency, and the like, is convenient for popularization and application, and has the application prospect of developing into an array multi-channel electrophoresis chip with integrated conductivity detection. The invention is widely applicable to the analysis detection of samples such as ionic compounds, biochemical samples, medicaments, pesticide residues, etc.

Description

1. Technical field [0001] The invention belongs to the technical field of the combination of microfluidic chip analysis and testing technology and micro-electromechanical system (MEMS) processing technology, and specifically relates to the structure of microfluidic composite electrophoresis chip. 2. Background technology [0002] The microfluidic chip integrates the basic operation units such as sample preparation, reaction, separation, and detection involved in the fields of chemistry and biology into a chip of several square centimeters, forming a network of separated micro-pipes, with controllable fluid running through the entire system . Since the volume of fluid in the pipeline of the microfluidic chip ranges from picoliters to nanoliters, and the amount of analyzed samples is small, high detection sensitivity and reproducibility are particularly important for microanalysis systems. Currently recognized detectors with high detection sensitivity mainly include laser-ind...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/00
Inventor 徐溢马亮波彭金兰梁静季金苟
Owner CHONGQING UNIV
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