Loop-mediated isothermal amplification assay kit and detection method of hand, foot and mouth disease
A detection kit and technology for hand, foot and mouth disease, applied in biochemical equipment and methods, microbiological measurement/inspection, etc., can solve the problems of time-consuming, laborious and cumbersome steps, and achieve simple operation methods, high sensitivity, and high accuracy Effect
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[0043] The following examples further illustrate the invention, but should not be construed as limiting the invention.
[0044] The kit contains the following components:
[0045] (1) Viral RNA extraction reagent
[0046] Viral RNA can be extracted using the conventional Trizol method, or viral RNA extraction kits produced by various biological companies.
[0047] (2) LAMP detection reaction solution
[0048] ①Reaction solution Cox A16-used to detect Coxsackie virus A16
[0049] Contains the following ingredients:
[0050] 10 x LAMP buffer (200mmol / L Tris-HCl, 100mmol / L KCl, 20mmol / L MgSO 4 , 100mmol / L (NH 4 )SO4 , 1.0%Tritonx-100); 100mmol / L MgSO 4 25mmol / LdNTP; 5mol / L betaine (betaine); 20umol / L primer CoxA16-FIP; 20umol / L primer CoxA16-BIP; 5umol / L primer CoxA16-F3; 5umol / L primer CoxA16-B3; DEPC treated water.
[0051] The primer sequences are:
[0052] CoxA16-FIP:
[0053] 5-AAGTRGGTTTCGGAGCCCCTTTTTTAGCCAAACCCAATGGTGAG-3
[0054] CoxA16-BIP:
[0055] 5-TTYGC...
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