Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Plant stress tolerance related protein GmTPRPK1, encoding gene thereof, and application thereof

A stress tolerance, gene technology, applied in the direction of plant genetic improvement, botanical equipment and methods, application, etc., can solve problems such as comprehensive improvement of stress resistance of difficult plants

Active Publication Date: 2011-11-09
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the stress tolerance of plants is a complex trait regulated by multiple genes, it is difficult to achieve comprehensive improvement of plant stress resistance by introducing a single functional protein gene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plant stress tolerance related protein GmTPRPK1, encoding gene thereof, and application thereof
  • Plant stress tolerance related protein GmTPRPK1, encoding gene thereof, and application thereof
  • Plant stress tolerance related protein GmTPRPK1, encoding gene thereof, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Cloning of embodiment 1, GmTPRPK1

[0048] 1. Isolation of mRNA

[0049] Seedlings of the soybean variety Tiefeng No. 8 (purchased from the Institute of Crop Science, Chinese Academy of Agricultural Sciences) grown in soil pots for about 20 days were quick-frozen in liquid nitrogen and stored at -80°C for later use.

[0050] Total RNA was extracted from soybean leaves by Trizol method (TianGen), and reverse transcriptase XL (AMV) was used for first-strand cDNA synthesis. The ds cDNA was synthesized by SMART method, and the PCR product was detected by 1.0% agarose gel electrophoresis.

[0051] 2. Acquisition of the full-length sequence of the GmTPRPK1 gene

[0052] The full-length sequence of the soybean dual-specificity protein kinase gene was obtained by 5'RACE and 3'RACE methods.

[0053] The protein shown in Sequence 1 of the sequence listing is named GmTPRPK1 protein, which consists of 492 amino acid residues and has a conserved dual-specificity protein kinase ac...

Embodiment 2

[0054] Example 2, real-time fluorescent quantitative PCR analysis of the expression characteristics of GmTPRPK1

[0055] 1. Coercion treatment

[0056] Seedling age is 20 days soybean variety Tiefeng No. 8 seedlings, carry out following treatment:

[0057] (1) Drought treatment ( figure 2 A): The potted soybean seedlings are taken out to absorb the moisture on the roots, placed on dry filter paper, and cultivated in a drought for 30 minutes, 1 hour, 2 hours, 5 hours, 12 hours, and 24 hours. After that, the materials are taken out and quickly frozen with liquid nitrogen. Store at -80°C for later use.

[0058] (2) Salt treatment ( figure 2 B): Soybean seedlings were placed in 200 mM NaCl solution, light cultured for 30 minutes, 1 hour, 2 hours, 5 hours, 12 hours, and 24 hours, and then the materials were taken out, quick-frozen with liquid nitrogen, and stored at -80°C for later use.

[0059] (3) Abscisic acid treatment ( figure 2 C): Soybean seedlings were placed in 200...

Embodiment 3

[0077] Example 3, GmTPRPK1 improves the drought tolerance of Arabidopsis

[0078] 1. Construction of recombinant expression vector

[0079] 1. Cloning of GmTPRPK1 gene

[0080] A pair of primers (GmTPRPK1-121F and GmTPRPK1-121R) were designed according to the sequence of the GmTPRPK1 gene, and SmalI and SpeI recognition sites were introduced at the ends of the primers respectively, and GmTPRPK1 was amplified by PCR using Tiefeng 8 soybean cDNA as a template.

[0081] GmTPRPK1-121F: 5'-TCCCGGGCGTGTGATTGCACTTCTCACCAATG-3';

[0082] GmTPRPK1-121R: 5'-TACTAGTCAATGAGGGGAAGTAGAAG-3'.

[0083] The PCR amplified product was subjected to 1.2% agarose gel electrophoresis, and a band of about 1.5 Kb was recovered and purified using Agarose Gel DNA Purification Kit Ver.2.0 (TaKaRa Company, Code No.: DV807A).

[0084] 2. Construction of recombinant expression vector

[0085] ①Use restriction endonucleases SmalI and SpeI to digest step 1 to recover the purified PCR product, and recover ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a plant stress tolerance related protein GmTPRPK1, an encoding gene thereof, and an application thereof. The protein provided by the invention is (a) or (b): (a) the protein is composed of an amino acid sequence represented by a sequence 1 in a sequence listing; and (b) the protein which is related to the plant stress tolerance, derived from the sequence 1, and obtained through substituting and / or deleting and / or adding to the amino acid sequence of the sequence 1 by one or some amino acid residues. The GmTPRPK1 expresses under the induction of drought, high salt, ABA,ethylene and the like, and the expression of the GmTPRPK1 is inhibited under the low temperature threat. The GmTPRPK1 can enhance the resistance of a plant to high salt and drought, and the growth ofroots. The GmTPRPK1 allows autophosphorylation to be carried out, and can be positioned on nucleuses and cell membranes, and the distribution range is large. The GmTPRPK1 of the present invention lays the foundation for artificially controlling the expression of the anti-stress and stress resistance related gene, and plays an important role in breeding plant varieties with strengthened anti-stress and stress resistance.

Description

technical field [0001] The invention relates to a plant stress tolerance-related protein GmTPRPK1, its coding gene and application. Background technique [0002] Adversity stresses such as drought, high salinity and low temperature are obstacles to plant growth and development. Therefore, understanding the response and signal transduction mechanism of wheat to stress conditions and improving the stress resistance of wheat varieties have become one of the important tasks of wheat genetic research and wheat variety improvement. [0003] Under adversity stress, plants will produce a series of responses, accompanied by many physiological, biochemical and developmental changes. Clarifying the response mechanism of plants to stress will provide scientific evidence for the research and application of stress-resistant genetic engineering. At present, the research on plant stress resistance has gradually penetrated into the cellular and molecular levels, combined with genetics and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
Inventor 徐兆师马有志李连城陈明
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com