Method for producing Hirsutella sinensis
A technology of Trichospora chinensis and a production method, which is applied in the production field of a new strain of Trichospermum chinensis in China, can solve the problems of environmental pollution and flammability caused by the discharge of artificial cordyceps sinensis fermentation waste liquid, and achieve enhanced controllability and increased yield. , the effect of reducing environmental pollution
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Embodiment 1
[0037] A kind of production method of Mortierella chinensis is carried out by following steps:
[0038] (1) Incline culture of semi-solid bacteria:
[0039] Dissolve the medium in boiling water, adjust the pH to 7.2, and sterilize at 121°C for 30 minutes to make a slant medium; after cooling, put it in an incubator at 28°C and cultivate it for 3 days. After confirming that there is no bacterial contamination, it is imported into China Mortierella species, evenly coated; controlled temperature 17 ~ 18 ℃ culture, culture time 40 days; then, put into 3 ℃ refrigerator for storage; wherein the composition of the slant medium is by weight percentage: defatted silkworm chrysalis powder 2.1%, corn Flour 1.5%, Bran 1.5%, Glucose 2%, KH 2 PO 4 0.02%, MgSO 4 0.01%, agar 1.8%, the rest is water; the inoculum amount is 15%;
[0040] (2) Shake flask cultivation of liquid bacteria:
[0041] Inoculate the strains cultivated on the slant into shake flasks with liquid medium, and culture t...
Embodiment 2
[0055] The same method as the embodiment is implemented through the following steps
[0056] (1) Incline culture of semi-solid bacteria:
[0057] Dissolve the medium in boiling water, adjust the pH to 7.2, and sterilize at 121°C for 30 minutes to make a slant medium; after cooling, put it in an incubator at 28°C and cultivate it for 3 days. After confirming that there is no bacterial contamination, it is imported into China Trichosporum species, evenly coated; controlled temperature 18 ~ 19 ° C culture, culture time 45 days; then, put into 4 ° C refrigerator for storage; wherein the composition of the slant medium is by weight percentage: defatted silkworm chrysalis powder 2.9%, corn Flour 1.5%, Bran 4.5%, Glucose 2%, KH 2 PO 4 0.02%, MgSO 4 0.01%, agar 1.8%, and the rest are water; wherein the inoculation amount is 15%; the insertion of Mortierella chinensis species is at 18°C, the inoculum amount is 10%, and it is flame-protected aseptic inoculation; wherein the defatted ...
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