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Application of ipomoea batatas Sporamin protein in preparation of medicines for treating and preventing tumours and health products

A sweet potato and protein technology, applied in antitumor drugs, drug combinations, peptide/protein components, etc., can solve the problems of research reports on tumor cell growth and metastasis without sweet potato Sporamin protein.

Active Publication Date: 2011-09-28
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no research report on the inhibition of tumor cell growth and metastasis by sweet potato Sporamin protein

Method used

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  • Application of ipomoea batatas Sporamin protein in preparation of medicines for treating and preventing tumours and health products
  • Application of ipomoea batatas Sporamin protein in preparation of medicines for treating and preventing tumours and health products
  • Application of ipomoea batatas Sporamin protein in preparation of medicines for treating and preventing tumours and health products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, prepare and purify sweet potato Sporamin protein from 55-2 sweet potato

[0032] Preparation of Sweet Potato Crude Protein:

[0033] Fresh potatoes (variety: 55-2, protein content 0.5-3.0g / 100g fresh weight) were cleaned, weighed, chopped, and then mixed with 0.1% NaHSO 3 50mmol·L -1 Soak in Tris-HCl buffer solution (pH 7.5) (1L·kg -1 fresh weight), beating, slurry centrifugation (3000g centrifugation 10min), take the supernatant, add ammonium sulfate to the supernatant under ice bath to 60% saturation, stand still, centrifugation (3000g centrifugation 30min), precipitate back to dissolve in Distilled water or 50mmol·L -1 The sweet potato Sporamin protein crude protein powder is obtained by freezing and drying in a Tris-HCl buffer solution, and the protein content is 40%-60%.

[0034] Purification of sweet potato sporamin protein:

[0035] Weigh an appropriate amount of sweet potato crude protein powder, add 100ml of distilled water to dissolve it, a...

Embodiment 2

[0039] Embodiment 2 prepares sweet potato Sporamin protein from red winter sweet potato

[0040] Preparation of Sweet Potato Crude Protein:

[0041] Cut the washed sweet potatoes (variety: 55-2, protein content 0.5-3.0g / 100g fresh weight) into small pieces of 2cm square, and put them in 0.5g / Kg sodium bisulfite ice solution to prevent them from browning. Change. Then grind the potato pieces into a pulp with a grinder and filter. The filtrate was centrifuged at 3000 g at room temperature for 30 min to precipitate the starch therein. Get the supernatant and adjust the pH to 4 with 2M hydrochloric acid, centrifuge at 3000g for 30min at room temperature; after diluting the precipitate with 10 times of 5% sodium chloride solution, adjust the pH to neutral with 2M sodium hydroxide; The molecular weight is more than 8000, and the sweet potato protein molecular weight is 25000 under reducing conditions) after dialysis and freeze-drying, dry sweet potato Sporamin protein powder is o...

Embodiment 3

[0043] Embodiment 3, the preparation of sweet potato Sporamin protein granule

[0044] In dry sweet potato Sporamin crude protein powder (Sporamin content is 55%), add the lactose of 0.2 times of quality, the microcrystalline cellulose of 0.1 times of quality, every 1000g adds 3~5g natural fruit flavor additive, 5~6g sweetener, After mixing evenly, moisten with 90% ethanol, form under pressure, and then granulate through a granulator to obtain granules. Packed in a composite aluminum foil bag.

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Abstract

The invention discloses a new use of ipomoea batatas Sporamin protein. According to the invention, ipomoea batatas Sporamin protein can be applied to prepare products for preventing and / or treating tumours and preparing inhibitors for the proliferation of eukaryote tumour cells. Tests of anticancer activity show that ipomoea batatas Sporamin protein is able to inhibit the proliferation and metastasis of SW480 and HCT-8 cells in human colorectal cancer cell lines and Lewis lung cancer cells, and also inhibit the invasion of the cells to a basement membrane. Thus ipomoea batatas Sporamin protein has an obvious anticancer effect. In addition, deriving from grain crop ipomoea batatas, ipomoea batatas Sporamin protein enjoys high security which ensures the long-term medication of patients. And having essential aminoacid that is reasonable in composition, ipomoea batatas Sporamin protein plays a role in nutrition improvement and can improve the health of cancer patients from multiple perspectives.

Description

technical field [0001] The invention relates to a new application of sweet potato Sporamin protein, in particular to the application of sweet potato Sporamin protein in the preparation of drugs for preventing and treating tumors and health care products. Background technique [0002] Sweet potato (Ipomoea batatas) is an annual or perennial herbaceous plant belonging to Convolvulaceae (Convolvulaceae). Sweet potato is rich in nutrition, rich in starch and soluble sugar, and also contains protein, fatty acid, multivitamins, polyphenols, calcium, phosphorus, iron and other minerals. Its protein content is about 1.2-10g / 100g dry matter, but the protein content may vary greatly among different varieties. [0003] Sporamin protein is a group of special proteins in sweet potato tubers. It was discovered by Maeshima et al. in 1985. It mainly exists in sweet potato tubers, accounting for 60% to 80% of sweet potato soluble protein, while almost none in other organs. It is a sweet po...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K36/8945A61P35/00C07K14/415
Inventor 木泰华李鹏高邓乐
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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