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Preparation and application method of filter paper collecting biological sample

A biological sample and filter paper technology, which is applied in the field of clinical disease diagnosis and detection, can solve the problems of low accuracy, low stability, and difficult elution of test results, and achieve the effects of easy elution detection, improved stability, and extended storage period

Inactive Publication Date: 2011-09-14
东莞南方医大代谢医学研发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] Disadvantages: Patients need to control their diet and visit the hospital in person. Blood sugar is easily affected by diet, exercise, emotion, sleep, and medication. It is not easy to collect and store blood samples. Multiple blood collections cause additional pain to patients
[0020] Disadvantages: Urine sugar is not used as a diagnostic indicator for diabetes, and is generally only used for monitoring the control of diabetes and an indicator that may indicate diabetes and requires further examination
[0025] After the blood is collected by the existing filter paper, the biological sample is easy to deteriorate and is not easy to store, and the sample is too much lost or even difficult to elute during elution, resulting in the disadvantages of low accuracy, low stability, and poor repeatability of the test results.
These deficiencies limit the popularization and application of filter paper in clinical diagnostic testing.

Method used

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  • Preparation and application method of filter paper collecting biological sample
  • Preparation and application method of filter paper collecting biological sample
  • Preparation and application method of filter paper collecting biological sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment 1. The filter paper of the present invention was used to collect blood samples, the blood samples were air-dried naturally, and after being placed at room temperature for 3 days, the samples were eluted and recovered. The glycated hemoglobin in the sample was detected by enzyme-linked immunosorbent assay. Three replicate holes were made for each sample. The chart shows that the coefficient of variation among the multiple wells is small, and the value is highly similar to that of the control A (results are shown in the appendix figure 2 ).

Embodiment 2

[0077] Embodiment 2. Blood samples were collected with the filter paper of the present invention, air-dried naturally, left at room temperature for 7 days, and the samples were eluted and recovered. The glycated hemoglobin in the sample was detected by enzyme-linked immunosorbent assay. Three replicate holes were made for each sample. The chart shows that the coefficient of variation among the multiple wells is small, and the value is highly similar to that of the control A (results are shown in the appendix image 3 ).

Embodiment 3

[0078] Embodiment 3. Blood samples were collected by using the filter paper of the present invention, air-dried naturally, left at room temperature for 11 days, and the samples were eluted and recovered. Then use the enzyme-linked immunosorbent assay to detect the glycated hemoglobin in the sample, and make three duplicate holes for each sample. The chart shows that the coefficient of variation among the multiple wells is small, and the value is highly similar to that of the control A (results are shown in the appendix Figure 4 ).

[0079] Ethylenediaminetetraacetic acid, commonly known as EDTA, is an organic compound. It is a hexadentate ligand that can combine with metal ions such as Mg2+, Ca2+, Mn2+, and Fe2+. Since the action of most nucleases and some proteases requires Mg2+, it can be used as an inhibitor of nucleases and proteases. Ethylenediaminetetraacetic acid is an important complexing agent with a wide range of uses, such as bleaching and fixing solutions, dyei...

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Abstract

The invention provides a preparation and application method of filter paper collecting a biological sample. An absorbing material with high accuracy, high stability and good repeatability is prepared by adding a specific stabilizer to the filter paper so that the sample is convenient to convey and store at normal temperature and easy to elute after drying, and the storage time of the biological sample is prolonged. According to the invention, a high-performance cellulose acetate film and a cellulose nitrate film are used as the filter paper materials; the filter paper material is completely soaked in 3.8 mM or 7.7 mM of aqueous solution of ethylene diamine tetraacetic acid; the completely soaked filter paper material is taken out and dried at 45 DEG C; and the finished product is taken out and stored in a sealed manner. The method provided by the invention is convenient for the detection process, and can lower the clinical examination cost and reduce economical burden on patients.

Description

technical field [0001] The invention belongs to the field of clinical disease diagnosis and detection, and relates to a filter paper for collecting liquid biological samples that can be used in clinical diagnosis and detection in a wide range of fields. Background technique [0002] Clinical testing is an important tool for disease diagnosis and prognosis, in order to accurately diagnose diseases and provide scientific basis for clinical treatment. Laboratory workers must continue to explore more accurate, more economical and practical testing methods in the process of practice, so as to more effectively assist clinical diagnosis and differential diagnosis. [0003] Clinical biological samples include: body fluids and tissues, specifically including blood, urine, saliva, lymph fluid, cerebrospinal fluid, gastrointestinal secretion fluid and various tissue sections. Due to the convenience of taking liquid biological samples, liquid biological samples such as blood and urine ...

Claims

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Application Information

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IPC IPC(8): D21H27/08D21H13/36
Inventor 惠宏襄赵小宁唐永明孙大力
Owner 东莞南方医大代谢医学研发有限公司
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