Preparation method of galactooligosaccharides

Abstract

The invention discloses a preparation method of galactooligosaccharides, which comprises the following steps: uniformly mixing thalli producing beta-galactosidase with sodium alginate solution to get mixed bacterial liquid; spraying and pressing the mixed bacterial liquid into calcium chloride solution, and soaking to get an immobilized matter; cutting up the immobilized matter, pouring into mixed solution of calcium chloride and chitosan, stirring and soaking; placing particles of the immobilized matter in glutaraldehyde solution for cross-linking, and cleaning to get the particles fixed with cells; and mixing the particles fixed with the cells with lactose solution, filtering after completing stirring reaction, and purifying syrup obtained by filtering so as to get the galactooligosaccharides. By adopting the method, the difficult problems of separation of the thalli, clarification of the syrup and the like in the way of directly using the thalli for performing transformation reaction can be effectively solved, and the production efficiency is improved; the production time is shortened, the production equipment is simplified, and the production cost is saved; and the consumptionof resources is reduced, the immobilized cells containing the beta-galactosidase can be utilized repeatedly, the frequency of fermentation and input into a tank can be reduced, and the pressure on environment friendliness can be reduced.

Description

technical field [0001] The invention relates to a preparation method of galactooligosaccharide. Background technique [0002] Galactooligosaccharide (GOS) is a functional oligosaccharide, which has good physiological effects and is an effective growth factor for Bifidobacterium and Lactobacillus. It can be widely used in various foods, especially infant formula dairy. The physiological effects of galactooligosaccharides are: (1) promote the proliferation of bifidobacteria and lactobacilli, inhibit the infection of pathogenic bacteria and the development of spoilage bacteria; (2) inhibit the metabolism of toxic substances and the production of harmful enzymes; (3) prevent constipation; (4) Improve serum lipids, promote the growth of volatile fatty acids, and reduce total serum cholesterol concentration; (5) Protect liver function; (6) Lower blood pressure; (7) Promote calcium absorption and prevent bone (8) Improving human immunity; (9) Preventing dental caries. Galacto-ol...

AI Technical Summary

Problems solved by technology

[0004] Purified β-galactosidase is expensive. Adding β-galactosidase directly to the reaction solution to produce galactooligosaccharides will greatly increase production costs. This method cannot achieve continuous production and seriously reduces production efficiency.

[0005] If the bacteria producing β-galactosidase are directly put into the lactose solution, galacto-oligosaccharides can be produced. In theory, flocculation can also be used to separate the bacteria, but in practice, it is difficult to control the separation of the experimental bacteria under the process conditions. , will introduce a large amount of foreign ions into the product, which will increase the ash content in the product, increase the cost of post-processing, and is not an economical production method