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Fast detection method of nitrofuran metabolites in aquatic products

A nitrofuran and detection method technology, which is applied in the field of rapid detection of nitrofuran metabolites in aquatic products, can solve the problems of poor repeatability of measurement results, easily affected enzyme activity by reaction conditions, and short life of ELISA reagents, etc., to achieve The effect of shortening the sample inspection time, easy promotion, and simple and fast operation

Inactive Publication Date: 2011-09-07
杭州南开日新生物技术有限公司
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Problems solved by technology

However, the activity of the enzyme in the ELISA method is easily affected by the reaction conditions, which easily leads to poor repeatability of the measurement results.
In addition, ELISA reagents have a short lifespan, so cryopreservation is required

Method used

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  • Fast detection method of nitrofuran metabolites in aquatic products
  • Fast detection method of nitrofuran metabolites in aquatic products
  • Fast detection method of nitrofuran metabolites in aquatic products

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Embodiment Construction

[0030] The preparation of the reagent plate of the present invention includes the preparation of carrier protein conjugates, the preparation of anti-nitrofuran metabolite monoclonal antibody, the preparation of colloidal gold solution, the preparation of colloidal gold-labeled anti-nitrofuran metabolite monoclonal antibody and the preparation of nitrofuran metabolite monoclonal antibody. Assembly of furan metabolite immunocolloidal gold rapid detection kit.

[0031] 1. Coupling of Haptens to Carrier Proteins

[0032] Synthesis of p-aldehyde benzoic acid derivatives of nitrofuran metabolites: Weigh 3g of p-aldehyde benzoic acid in 10mL distilled water, add dimethylformamide (DMF) dropwise until the p-aldehyde benzoic acid is completely dissolved, add 1.0g of nitrofuran metabolites (furazolidone metabolites, nitrofurantoin metabolites, nitrofurazone metabolites or furaltadone metabolites), react at room temperature for 2 hours, and wash with filtered water to obtain a white soli...

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Abstract

The invention relates to a fast detection method of nitrofuran metabolites in aquatic products, specifically relating to a fast detection method for detecting furazolidone metabolites, furantoin metabolites, furacillin metabolites and furaltadone metabolites in aquatic products. An immune colloidal gold fast detection reagent board is prepared in the invention; the reagent board comprises upper and lower plastic templates and a backing; and a sample pad, a colloid gold combining pad, a nitrocellulose membrane and an absorbent pad are tightly attached on the backing in turn. The nitrocellulose membrane is sprayed with a detection line and a quality control line in a direction from the sample pad to the absorbent pad in turn; and the reaction result can be represented with a macroscopic colour. The reagent board can perform semi-quantitative visual detection; the whole operation procedure only needs 2 hour; and expensive experimental auxiliary facilities are not needed. The method is in favour of screening massive samples, and is suitable for performing massive fast detection of nitrofuran metabolites in aquatic products by industrial and commercial administrations, disease inspection organizations, and aquaculture and processing enterprises.

Description

technical field [0001] The invention relates to a rapid detection method for detecting nitrofuran metabolites in aquatic products, in particular to a rapid detection method for detection of furazolidone metabolites, nitrofurantoin metabolites, nitrofurazone metabolites and furaltadone metabolites in aquatic products. Background technique [0002] Nitrofuran drugs are a class of synthetic antibacterial drugs, mainly referring to furazolidone, nitrofurantoin, nitrofurazone and furaltadone. Nitrofuran drugs have killing effects on most Gram-positive bacteria and Gram-negative bacteria, fungi and protozoa and other pathogens, and are low in price and good in efficacy. They were once widely used in aquaculture. Large-dose or long-term use of nitrofuran drugs can produce toxic effects and "three-induced" effects on farmed animals. The "three-causing" effects refer to carcinogenic, teratogenic, and mutagenic effects. Nitrofuran compounds are direct mutagens, which can cause bacte...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 张少恩桑丽雅周崇盈
Owner 杭州南开日新生物技术有限公司
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