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Method for preparing high-purity rice protein and acquired products by using same

A rice protein, high-purity technology, applied in the field of high-purity rice protein, high-purity rice protein preparation, can solve problems such as low protein extraction rate, reduced solubility, and protein denaturation

Active Publication Date: 2011-08-17
WUXI JINNONG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of this method is simple, but because the protein in the rice dregs obtained from the preparation of rice starch sugar has already been denatured due to high temperature, the solubility in alkali solution is greatly reduced, resulting in a very low protein extraction rate, making the alkali-dissolving and acid-precipitating method in this does not apply

Method used

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  • Method for preparing high-purity rice protein and acquired products by using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1: The present invention prepares the method for high-purity rice protein

[0082] The steps of this method are as follows:

[0083] (1) pulping and refining

[0084] Add 5.25 kg of water to 1 kg of rice dregs with a protein weight percentage of 41.3%, to obtain 16% rice milk by weight of rice dregs, grind the rice milk with a colloid mill, and then filter through a 200-mesh sieve to obtain a filtrate.

[0085] (2) Degreasing

[0086] The filtrate obtained in step (1) was heated to 65°C, and then 40% weight concentration of sodium hydroxide aqueous solution was added to adjust the pH value of the filtrate to 8.2, and then soaked at 63°C for 30 minutes to saponify fat and swell protein.

[0087] (3) Enzymolysis reaction

[0088] The solution of step (2) was incubated at 63° C. for 30 minutes, adjusted its pH value to 4.2 with 1 mol / L hydrochloric acid solution, and then added 0.2% glucoamylase based on the mass of rice dregs. The enzyme activity of the gluco...

Embodiment 2

[0096] Example 2: The present invention prepares the method for high-purity rice protein

[0097] The steps of this method are as follows:

[0098] (1) pulping and refining

[0099]Add 5.06 kg of water to 1 kg of rice dregs with a protein weight percentage of 42.0%, to obtain rice milk of 16.5% by weight of rice dregs, grind the rice milk with a colloid mill, and then filter through a 150-mesh sieve to obtain a filtrate.

[0100] (2) Degreasing

[0101] The filtrate obtained in step (1) was heated to 64°C, and then 45% aqueous sodium hydroxide solution was added to adjust the pH value of the filtrate to 8.3, and then soaked at 60°C for 30 minutes to saponify the fat and swell the protein.

[0102] (3) Enzymolysis reaction

[0103] The solution of step (2) was incubated at 60° C. for 40 minutes, adjusted its pH value to 4.3 with 1 mol / L hydrochloric acid solution, and then added 0.1% glucoamylase based on the mass of rice dregs. The enzyme activity of the glucoamylase was ...

Embodiment 3

[0111] Example 3: The present invention prepares the method for high-purity rice protein

[0112] The steps of this method are as follows:

[0113] (1) pulping and refining

[0114] Add 4.9 kg of water to 1 kg of rice dregs with a protein weight percentage of 40.7%, to obtain 17% rice milk by weight of rice dregs, grind the rice milk with a colloid mill, and then filter through a 250-mesh sieve to obtain a filtrate.

[0115] (2) Degreasing

[0116] The filtrate obtained in step (1) was heated to 63° C., and then 35% by weight aqueous sodium hydroxide solution was added to adjust the pH value of the filtrate to 8.5, and then soaked at 65° C. for 30 minutes to saponify fat and swell protein.

[0117] (3) Enzymolysis reaction

[0118] The solution of step (2) was incubated at 65° C. for 20 minutes, adjusted its pH value to 4.5 with 1 mol / L hydrochloric acid solution, and then added 0.3% glucoamylase based on the mass of rice dregs. The enzyme activity of the glucoamylase was...

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Abstract

The invention relates to a method for preparing high-purity rice protein. The method comprises the following steps: sizing mixing and grinding, degreasing, enzymolysis reaction, separating washing and blanching and sterilization. The invention also relates to the high-purity rice protein acquired by using the method, wherein the rice protein contains following components by weight percent: more than 80% of protein, less than 3% of fat, and less than 3% of ash content. The protein extraction ratio is more than 80% and the additional value of rice residue is greatly increased.

Description

【Technical field】 [0001] The invention belongs to the technical field of deep processing of rice. More specifically, the present invention relates to a method for preparing high-purity rice protein, and also relates to the high-purity rice protein obtained according to the preparation method. 【Background technique】 [0002] The content of rice protein in rice is about 7%, which is mainly composed of albumin, globulin, prolamin and glutenin. Rice protein is a high-quality vegetable protein with high nutritional value. Its amino acid composition ratio is reasonable, which is similar to the optimal ratio mode of protein amino acid recommended by FAO / WHO. The amino acid score is as high as 67, the digestibility is 82, the biological value is 77, and the efficacy ratio is 1.7. The biological value (BV) and protein utility ratio (PER) of rice protein are almost the best among plant proteins. Therefore, rice protein is an important protein source in people's diet, and its nutrit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/12
Inventor 于秋生
Owner WUXI JINNONG BIOTECH CO LTD
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