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Cell lysis solution for extracting animal DNA, kit and method

A cell lysate and kit technology, applied in the field of DNA extraction, can solve the problems of high cost, poor quality, low DNA content, etc., and achieve the effect of high success rate, better effect, and high DNA yield

Inactive Publication Date: 2011-08-10
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a cell lysate for animal DNA extraction, a test kit containing the lysate and a method for extracting animal DNA using the kit, so as to solve the problem of high cost of the existing animal DNA extraction method and the The problem of low DNA content and poor quality

Method used

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  • Cell lysis solution for extracting animal DNA, kit and method
  • Cell lysis solution for extracting animal DNA, kit and method

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Experimental program
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Effect test

Embodiment 1

[0031] Prepare the cell lysate for animal DNA extraction according to the following recipe:

[0032] Tris-base 24g / L, EDTA 0.7g / L, SDS 24g / L, NaCl 7.52g / L, urea 120g / L, the rest is sterile water, adjust the pH value to 8.0 with NaOH.

Embodiment 2

[0034] Prepare the cell lysate for animal DNA extraction according to the following recipe:

[0035] Tris-base 20g / L, EDTA 1.0g / L, SDS 30g / L, NaCl 5g / L, urea 150g / L, the balance is sterile water, adjust the pH value to 8.5 with NaOH.

Embodiment 3

[0037] Prepare the cell lysate for animal DNA extraction according to the following recipe:

[0038] Tris-base 30g / L, EDTA 0.5g / L, SDS 20g / L, NaCl 10g / L, urea 100g / L, the rest is sterile water, adjust the pH value to 9.0 with NaOH.

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Abstract

The invention provides cell lysis solution for extracting animal DNA, a kit containing the lysis solution and a method for extracting animal DNA by using the kit. The cell lysis solution provided by the invention comprises Tris-base at a concentration of 20 to 30g / L, ethylene diamine tetraacetic acid (EDTA) at a concentration of 0.5 to 1.0g / L, sodium dodecyl sulfonate (SDS) at a concentration of 20 to 30g / L, NaCl at a concentration of 5 to 10g / L, urea at a concentration of 100 to 150g / L and the balance of sterile water, and the pH value of the cell lysis solution is regulated by NaOH to 8.0 to 9.0. When the animal DNA extraction kit provided by the invention is used, the extraction time is reduced by at least half hour compared with that in the prior art, the use of chloroform with strong toxic odor is avoided, the damage to the health of operators in an operation process is relieved, and the extracted DNA is high in yield and purity. The method provided by the invention is quick, simple, convenient, efficient and very repeatable. In the invention, the result is very stable, the success rate is high, extraction cost is reduced and large-scale extraction of DNA is easy.

Description

technical field [0001] The invention relates to the technical field of DNA extraction, in particular to a cell lysate, a kit and a method for animal DNA extraction. Background technique [0002] Since Watson and Crick proposed the DNA double helix model in the 1950s, molecular biology has achieved unprecedented development in breadth and depth. Our research on the molecular field has penetrated into various aspects such as biology, medicine, genetics and zoology. The extraction of genomic DNA is the most basic technology in molecular biology research and the guarantee for the success of follow-up experiments (see "Chicken Genome A Comparative Study of Different DNA Extraction Methods", Ma Xinhong et al.; Journal of Jiangxi Agricultural University, 2010.32(001): p.181-184). At the same time, the use of live blood collection and then DNA extraction from the blood can conduct polymorphism research and early selection of genomic DNA without killing animals, which is a very good...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 胡耀东朱庆兰丹吴华莉邱芙寒
Owner SICHUAN AGRI UNIV
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