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Method for extracting polyphenols from Siberian nitraria peels

A technique for the peel and polyphenols of white thorn, which is applied in the biological field to achieve the effects of improving extraction rate, high extraction rate and high extraction efficiency

Inactive Publication Date: 2011-07-06
肖国华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional extraction methods are Soxhlet extraction method, hot water extraction method and organic solvent reflux method, etc., but the pigment can only be extracted from the peel of Nitraria thorn or the production of low value-added products

Method used

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  • Method for extracting polyphenols from Siberian nitraria peels
  • Method for extracting polyphenols from Siberian nitraria peels

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting polyphenols from Nitraria pericarp of the present invention comprises the following steps:

[0025] (1) Cleaning: Wash the Nitraria rind with running water to remove sediment, polysaccharides and water-soluble impurities;

[0026] (2) Degreasing: Nitraria pericarp contains certain lipid substances, which affect the extraction effect of polyphenols, so the Nitraria pericarp should be degreased before extraction to remove lipid substances. The method adopts organic solvents such as ether and petroleum ether to degrease;

[0027] (3) Microwave-assisted extraction: Dry the degreased Nitraria pericarp at low temperature, and conduct auxiliary extraction for 100 seconds at a microwave frequency of 2450MHZ and a microwave power of 100W;

[0028] (4) Ultrasonic extraction: The Nitraria rind peel extracted with microwave assistance is extracted for 100 minutes at an ultrasonic frequency of 25KHZ, and the extraction temperature is 90°C;

[0029] (5) Impu...

Embodiment 2

[0032] A method for extracting polyphenols from Nitraria pericarp of the present invention comprises the following steps:

[0033] (1) Cleaning: Wash the Nitraria rind with running water to remove sediment, polysaccharides and water-soluble impurities;

[0034] (2) Degreasing: Nitraria pericarp contains certain lipid substances, which affect the extraction effect of polyphenols, so the Nitraria pericarp should be degreased before extraction to remove lipid substances. The method adopts organic solvents such as ether and petroleum ether to degrease;

[0035] (3) Microwave-assisted extraction: Dry the degreased Nitraria pericarp at low temperature, and conduct auxiliary extraction for 80 seconds at a microwave frequency of 2450MHZ and a microwave power of 500W;

[0036] (4) Ultrasonic extraction: extract the Nitraria pericarp assisted by microwave extraction for 80 minutes at an ultrasonic frequency of 50KHZ, and the extraction temperature is 70°C;

[0037] (5) Impurity remova...

Embodiment 3

[0040] A method for extracting polyphenols from Nitraria pericarp of the present invention comprises the following steps:

[0041] (1) Cleaning: Wash the Nitraria rind with running water to remove sediment, polysaccharides and water-soluble impurities;

[0042] (2) Degreasing: Nitraria pericarp contains certain lipid substances, which affect the extraction effect of polyphenols, so the Nitraria pericarp should be degreased before extraction to remove lipid substances. The method adopts organic solvents such as ether and petroleum ether to degrease;

[0043] (3) Microwave-assisted extraction: Dry the degreased Nitraria pericarp at low temperature, and conduct auxiliary extraction for 50 seconds at a microwave frequency of 2450MHZ and a microwave power of 800W;

[0044] (4) Ultrasonic extraction: The Nitraria rind peel extracted with the aid of microwaves was extracted for 20 minutes at an ultrasonic frequency of 80KHZ, and the extraction temperature was 40°C;

[0045](5) Impu...

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PUM

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Abstract

The invention relates to biotechnology, particularly relates to a method for extracting polyphenols from Siberian nitraria peels. The method comprises the following steps: (1) cleaning; (2) degreasing; (3) performing microwave-assisted extraction; (4) performing ultrasonic extraction; (5) removing impurities; (6) testing by determining contents of polyphenols (counted with content of proanthocyanidin) using sulfuric acid-vanillin method, hydrochloric acid-vanillin method and so on. The invention provides a set of sound technical scheme for extracting polyphenols from Siberian nitraria peels. Compared with the conventional extraction method, the method provided by the invention has the advantages of high extraction rate, high safety and simple operation, increases added value of product, and to a great extent, provides a prerequisite for large-scale processing of Siberian nitraria peels.

Description

technical field [0001] The invention relates to biotechnology, in particular to a method for extracting polyphenols from the peel of Nitraria thorn. Background technique [0002] Polyphenols refer to a group of chemical elements in plants, named after having multiple phenolic groups. Polyphenols have a strong antioxidant effect, among which proanthocyanidins (OPC) are the most efficient antioxidants found in plant sources so far. The French Academy of Sciences has done experiments on the scavenging free radical activity of polyphenols, and the results believe that proanthocyanidins (OPC) are one of the most effective free radical scavenger found so far. Its antioxidant activity is 20 times that of vitamin C and 50 times that of vitamin E. Polyphenols have the functions of protecting cardiovascular and preventing high blood pressure, anti-oxidation and anti-enzyme; they can reduce cholesterol and low-density lipoprotein levels, prevent thrombosis, and help prevent cardiov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K131/00
Inventor 张东宋永朋林辉史雪林崔庆峰葛岩涛申娟萍单斌马华丽
Owner 肖国华
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