Method for efficiently inducing jujube tree 2n pollen
A technology for pollen and jujube trees, applied in the directions of botanical equipment and methods, applications, and plant genetic improvement, can solve problems such as unsatisfactory induction effects, and achieve the effects of simple and convenient methods, high induction efficiency, and increased occurrence probability.
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[0014] Using the excellent diploid variety 'Honey Pot' as the test material, on May 12-13, 2010, a healthy jujube tree with normal development on the periphery of the canopy was selected, and the base was cut with a blade, with a length of about 0.8-1.2 mm and a depth of 0.8-1.2 mm. The xylem, the wound is smooth and has no burrs; quickly cover the wound with absorbent cotton, fix the absorbent cotton on the wound with plastic wrap to make the wound fully contact with the absorbent cotton, and wrap the plastic wrap with kraft paper for shading; use a syringe to inject 0.3% colchicine (containing 1% DMSO) was added dropwise on the absorbent cotton to soak the absorbent cotton; every 4-6 hours, the liquid medicine was dripped once to keep the absorbent cotton moist. After 24 hours, all wrappings were removed, and the wound was rinsed with water; collected and processed from June 1st to 7th. For flower buds that have reached the stage of bud splitting, use a dissecting needle to q...
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