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Method for detecting amoxicillin residual quantity in milk and meat product

A technology for amoxicillin and residues, applied in the field of detection of amoxicillin residues in milk and meat products, can solve the problems of unsatisfactory fluorescence detection stability, complicated operation steps, low detection sensitivity, etc., and achieves no special technology. Requirements, simple operation method, high sensitivity detection effect

Inactive Publication Date: 2011-05-25
云南健牛环境监测有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods have their own strengths in terms of detection precision, and some have low sensitivity and poor selectivity; Inconvenience in practical application
The inventor described a similar detection method in the published patent 201010109375.3, but there are also shortcomings such as low detection sensitivity and unsatisfactory fluorescence detection stability.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Prepare 500 mL of amoxicillin standard aqueous solution with a concentration of 80 mg / L, and dilute it step by step when used.

[0024] 2. Take 1 mL of amoxicillin standard aqueous solution with a concentration of 5 μg / mL and place it in a 10 mL colorimetric tube, add 1 mL of 2% Tween-20 aqueous solution, 1 mL of pH 6 buffer solution and 0.5 g / L of 2,3-dichloro -5,6 Dicyanobenzoquinone 2mL, dilute to the mark with water, shake well, and place at room temperature for 15min to obtain a charge transfer complex.

[0025] 3. Scan the above-mentioned charge-transfer complex solution in the wavelength range of 220-650nm, measure the fluorescence intensity at the maximum excitation wavelength of 338nm and the maximum emission wavelength of 418nm, and make a reagent blank at the same time.

[0026] 4. Take 1 mL of amoxicillin standard solutions with concentrations of 0.01, 1.00, 5.00, 10.00, 20.00 and 50.00 μg / mL, add 1 mL of 2% Tween-20 aqueous solution, 1 mL of pH6 buffer s...

Embodiment 2

[0033] 1. Prepare 100mL of amoxicillin standard aqueous solution with a concentration of 50mg / L, and dilute it step by step when used.

[0034] 2. Take 1mL of amoxicillin standard aqueous solution with a concentration of 10μg / mL and place it in a 10mL colorimetric tube, add 1mL of 1% Triton-110 aqueous solution, 2mL of pH7 buffer solution and 1g / L tetracyano for the second time Dilute 1 mL of methylbenzoquinone to the mark with water, shake well and place at room temperature for 10 min to obtain a charge-transfer complex.

[0035] 3. Scan the above-mentioned charge-transfer complex solution in the wavelength range of 220-650nm, measure the fluorescence intensity at the maximum excitation wavelength of 310nm and the maximum emission wavelength of 430nm, and make a reagent blank at the same time.

[0036]4. Take 1 mL of amoxicillin standard solutions with concentrations of 0.01, 1.00, 5.00, 20.00, 40.00 and 80.00 μg / mL, add 1 mL of 1% Triton-110 aqueous solution, 2 mL of pH7 buf...

Embodiment 3

[0040] 1. Prepare 100 mL of amoxicillin standard aqueous solution with a concentration of 20 mg / L, and dilute it step by step when used.

[0041] 2. Take 1.0 μg / mL amoxicillin standard aqueous solution 1mL and place it in a 10mL colorimetric tube, add 1mL of 0.5% Tween-80 aqueous solution, 2mL of pH8 buffer solution and 2mL of 0.8g / L chloranil, Dilute the volume to the mark with water, shake well and place at room temperature for 10 min to obtain the charge transfer complex.

[0042] 3. Scan the above-mentioned charge-transfer complex solution in the wavelength range of 220-650nm, measure the fluorescence intensity at the maximum excitation wavelength of 335nm and the maximum emission wavelength of 460nm, and make a reagent blank at the same time.

[0043] 4. Take 1 mL of amoxicillin standard solutions with concentrations of 0.01, 1.00, 5.00, 10.00, 20.00, and 40.00 μg / mL, add 1 mL of 0.5% Tween-80 aqueous solution, 2 mL of pH8 buffer solution and 0.8 g / L of four Chlorobenzoq...

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Abstract

The invention discloses a method for detecting amoxicillin residual quantity in milk and meat products. The method includes the following steps: preparing amoxicillin standard aqueous solution; adding a nonionic surfactant; reacting the obtained solution with quinone or alizarin to get a charge transfer system; determining the wavelength scanning range of fluorescence or ultraviolet; further calculating the linear range, working curve and other parameters of the standard solution; processing the sample to be detected by corresponding charge transfer reaction; determining reaction parameters under ultraviolet or fluorescence; and calculating to obtain the amoxicillin residue quantity. The technology further enhances the value of fluorescence intensity or UV absorption by utilizing the sensitizing effect of non-ionic surfactants, thus meets the low-residue testing requirement; simultaneously improves the system stability significantly, makes up the disadvantage of poor detection stability of fluorescence in the earlier application, and is more feasible in the actual detection.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and relates to a method for detecting residual antibiotics in animal source food, in particular to a method for detecting residual amoxicillin in milk and meat products. Background technique [0002] Foods of animal origin such as milk and meat products generally contain rich nutrients such as protein, fat, amino acid, sugar, salt, calcium, phosphorus, iron, etc. The proportion is also increasing. Amoxicillin is widely used in animal husbandry production for the prevention and treatment of livestock and poultry diseases because of its cheapness, multiple dosage forms, broad antibacterial spectrum, high curative effect, and short course of treatment. The extensive use of amoxicillin has resulted in its residues in most animal-sourced foods. After people eat animal-sourced foods with excessive amoxicillin residues, it will cause harm to human health. The most common such as amoxicil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/25
Inventor 杨亚玲徐兴志耿卫东
Owner 云南健牛环境监测有限公司
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