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Ginseng saponin H extract and preparation method thereof

A technology of ginsenosides and total ginsenosides, which is applied in the direction of drug combinations, pharmaceutical formulas, plant raw materials, etc., can solve problems such as poor effects, and achieve the effects of novel use, convenient operation, and high purity

Inactive Publication Date: 2011-05-18
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The effect of separating ginsenoside H in the prior art is not good. During the research process of the ginsenoside H extract, the inventor adopted a new separation technology to test out a group of new ginsenoside H extracts and their preparation. method, these ginsenoside H extracts have high curative effect, high purity, good absorption, stable quality, and at the same time the preparation method has good separation effect, high content, simple process, convenient operation, low cost, and is suitable for industrial production

Method used

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  • Ginseng saponin H extract and preparation method thereof
  • Ginseng saponin H extract and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0046] The preparation of embodiment 1 ginsenoside H extract

[0047] Step 1. Extract American ginseng leaves with water twice, each time for 2 hours, add 10 times of water each time, mix the extract, concentrate to a relative density of 1.18, add 95% ethanol for alcohol precipitation, so that the alcohol content reaches 75%, and place More than 12h, filter, and concentrate the supernatant to dryness to obtain total saponins;

[0048] Step 2, the total saponins obtained in step 1 are dissolved in acetic acid buffer solution with a pH of about 5, add β-glucosidase, and react at 40°C. After the reaction is completed, add water to dilute the reaction solution and stir evenly to precipitate and collect the precipitate to obtain ginseng Total glycoside crude product;

[0049] Step 3. The crude product of total ginseng glycosides is dissolved by heating with ethanol, mixed into 2 times the amount of silica gel, subjected to silica gel column chromatography, and eluted with ethyl ac...

Embodiment 2

[0050] The preparation of embodiment 2 ginsenoside H extract

[0051] Step 1. Extract American ginseng with water twice, each time for 2 hours, add 10 times the amount of water each time, mix the extract, concentrate to a relative density of 1.18, add 95% ethanol for alcohol precipitation, so that the alcohol content reaches 75%, and place it for 12 hours above, filtered, and the supernatant was concentrated to dryness to obtain total saponins;

[0052] Step 2, the total saponins obtained in step 1 are dissolved in acetic acid buffer solution with a pH of about 5, add β-glucosidase, and react at 40°C. After the reaction is completed, add water to dilute the reaction solution and stir evenly to precipitate and collect the precipitate to obtain ginseng Total glycoside crude product;

[0053] Step 3. The crude product of total ginseng glycosides is dissolved by heating with ethanol, mixed into 2 times the amount of silica gel, subjected to silica gel column chromatography, and e...

Embodiment 3

[0054] The preparation of embodiment 3 ginsenoside H extract

[0055] Step 1. Extract Panax notoginseng with water twice, 2 hours each time, add 10 times of water each time, mix the extract, concentrate to a relative density of 1.18, add 95% ethanol for alcohol precipitation, so that the alcohol content reaches 75%, and place More than 12h, filter, and concentrate the supernatant to dryness to obtain total saponins;

[0056] Step 2, the total saponins obtained in step 1 are dissolved in acetic acid buffer solution with a pH of about 5, add β-glucosidase, and react at 40°C. After the reaction is completed, add water to dilute the reaction solution and stir evenly to precipitate and collect the precipitate to obtain ginseng Total glycoside crude product;

[0057] Step 3. The crude product of total ginseng glycosides was dissolved by heating with ethanol, mixed into 2 times the amount of silica gel, subjected to silica gel column chromatography, and eluted with ethyl acetate con...

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Abstract

The invention relates to a traditional Chinese medicine extract and a preparation method thereof, in particular to a Ginseng saponin H extract and a preparation method thereof. In the extract, the content of Ginseng saponin Rh1 is between 1 and 15 percent, the content of Ginseng saponin Rh2 is between 10 and 30 percent, the content of the Ginseng saponin Rh1 and the Ginseng saponin Rh2 is not less than 25 percent and the content of protodioscin is not less than 50 percent based on that of the Ginseng saponin Rh2.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract and its preparation, in particular to a ginsenoside H extract and its preparation. Background technique [0002] Ginseng has many reports in the treatment of cardiovascular and cerebrovascular diseases. [0003] Ginsenoside can treat coronary heart disease and ischemic stroke. [0004] Ginsenoside H is the secondary saponin produced by the hydrolysis of ginsenoside components, and then purified by silica gel column chromatography to obtain the effective part-H group saponin. Its components are ginsenosides Rh1, Rh2 and Rh3, of which Rh2 is the The main ingredient of the drug is also the main anticancer active ingredient of the drug. Pharmacodynamic studies have shown that: ginsenoside Rh2 has a significant and specific inhibitory effect on the proliferation of cancer cells such as liver cancer, melanoma B16, cervical cancer and sarcoma S180. Its mechanism of action is as follows: in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61P35/00A61K31/704
Inventor 宋兆辉张兰兰蔡楠林万里黄芝娟周水平朱永宏
Owner TIANJIN TASLY PHARMA CO LTD
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