Method for constructing lung tumor experimental animal model

Abstract

The invention provides a method for constructing a lung tumor experimental animal model. The method comprises the following steps of: 1, feeding a dog with cyclosporin according to the weight ratio of the dog to cyclosporin of 10mg to 1kg 4 days before inoculation; 2, preparing canine transmissible venereal tumor (CTVT) tissue blocks; and 3, puncturing to a predetermined target part by using a 16G guide puncture needle, placing 2 to 3 CTVT tissue blocks into the puncture needle, arranging a sterile gelatin sponge strip behind the CTVT tissue blocks, pushing the tissue blocks into lung tissues by a needle core and pulling the needle out. In the method, CTVT cells are used as tumor strains, the tumor tissue blocks are prepared through subcutaneous tumor cell passage by using the characteristics of transplantability and genetic stability; in-lung planting is performed by a minimally invasive intervention method to establish a dog transplantable lung cancer model; and the model can be applied to related experimental researches such as growth, invasion, transfer and the like of large-sized animal lung tumors.

Description

technical field [0001] The invention relates to the field of constructing experimental animal models of diseases, in particular to a method for constructing experimental animal models of lung tumors. Background technique [0002] In recent years, with the widespread application of interventional minimally invasive techniques such as arterial chemoembolization, percutaneous ablation (radiofrequency, microwave, laser, cryo) and percutaneous radioactive seed implantation in lung tumors, most patients with advanced The hope of controlling the lesion and even curing it. However, the basic experimental research of related interventional techniques is relatively lagging behind. One of the main reasons is that the common animal models of lung tumors at home and abroad are mostly used in the study of etiology and pathogenesis, such as chemically induced models, transgenic mouse models and tumor models. Xenograft models, etc. [0003] Different experimental models are suitable for d...

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Problems solved by technology

For example, in the experiment of foreign Ahrar, tissue serous fluid was used. The tissue serous fluid or cell suspension can be implanted by percutaneous puncture with a thinner needle (20-22G). Diffusion and pleural implantation, etc.

[0009] The second method is thoracotomy and implantation of tissue blocks. This method is complicated to operate and relatively traumatic. Complications such as pneumothorax and hemothorax are prone to occur. In mild cases, the tumor formation rate is affected, and in severe cases, it is life-threatening, resulting in a low success rate of animal experiments. high

[0010] The third method is percutaneous puncture implantation of tissue blocks. In theory, this method can effectively avoid pleural implantation caused by cell reflux, but the puncture needle required is relatively thick, and its success rate and complications have not been reported at home and abroad.

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