Expanded bed chromatographic separation column used for biochemical separation process and process flow

Abstract

The invention relates to an expanded bed chromatographic separation column used for a biochemical separation process and a process flow, belonging to the field of biochemistry. In the invention, a liquid pressure equalizing distributor with self-cleaning function is arranged below a porous blocking sieve plate at the bottom of an expanded bed, and an upper nozzle assembly with back-flushing cleaning function is arranged at the top of the expanded bed so that the expanded bed chromatographic separation column is suitable for treating a raw material liquid containing solid particle impurities, meanwhile, the raw material sample liquid entering the expanded bed can be better distributed uniformly, therefore, the problem of uniform distribution of feed liquid pressure of lower-bed pressure drop feed liquid along a radius direction in the industrial amplification production process is solved, fluid passing through a bed layer of the expanded bed is better ensured to be in a plug flow state, the separation theoretical plate number and the chromatographic separation efficiency of the expanded bed can be greatly enhanced, the using effect and the using efficiency of the expanded bed are improved and the adsorption capacity and the desorption resolution of the expanded bed are enhanced.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a chromatographic separation device used for extraction / separation / purification in industrial scale production and a production process thereof. Background technique [0002] Chromatographic separation is one of the most commonly used methods in biochemical separation processes. [0003] The traditional chromatographic separation column (referred to as chromatographic column) adopts the form of fixed bed, that is, the adsorption resin is pressed and fixed in the chromatographic column with upper and lower porous sieve plates, so that it cannot flow, commonly known as "fixed bed". [0004] The characteristics of the traditional fixed bed are that due to the small porosity of the bed, the resistance of the fluid passing through the bed is relatively large, the fluid flows through the bed in a "plug flow", and the fluid velocity in the radial direction is well distr...

AI Technical Summary

Problems solved by technology

In order to achieve the goal of uniform pressure distribution, the volume of the cavity of the expanded bed must be large, but the increase in the volume of the cavity will lead to serious back-mixing and affect the use effect / efficiency of the expanded bed;

[0026] 2) In the middle and late stages of the "sample loading" process, as the solid content in the circulating sample solution increases, the solid particles coalesce. At this time, the circulating sample solution with high viscosity and high solid content is difficult to clean the porous screen. The role of the support plate, the clogging phenomenon will still occur, and it is difficult to avoid

[0027] Therefore, until now, this technical solution has not been practically applied in actual industrial production, nor has there been a corresponding commercial product.

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