Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Drug composition with anti-tumor effect

A technology with anti-tumor effect and anti-inflammatory effect, which is applied in the direction of anti-tumor drugs, drug combinations, active ingredients of hydroxyl compounds, etc. It can solve problems such as difficult to use anti-tumor drugs, cancer synergies are not often found, and achieve inhibition Tumor growth, anti-tumor effect on tumor growth

Inactive Publication Date: 2010-12-15
NANJING NORMAL UNIVERSITY
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it also has similar problems as non-steroidal anti-inflammatory drugs, so it is difficult to use it as an anti-tumor drug in the clinical line
[0009] Due to the above problems, these substances cannot obtain satisfactory therapeutic effects. Therefore, it is necessary to improve the effect of the above substances on tumor cell growth inhibitory activity, expand their anti-tumor range (anti-tumor spectrum), and / or provide a A method to reduce the dose of chemotherapeutic agents to reduce the duration of administration and / or reduce potential or harmful side effects to patients
Despite this need, synergies are not often found in the treatment of cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Drug composition with anti-tumor effect
  • Drug composition with anti-tumor effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Combination of Elemene and Celecoxib on Human Hep G 2 inhibition of cells

[0039] Material :

[0040] Cell line Human liver cancer cell line Hep G 2 (purchased from Shanghai Cell Bank)

[0041] Drug solution preparation: Elemene is dissolved in DMSO before use, and then added to cultured cells to prepare the desired final concentration of the drug. Celecoxib was dissolved in DMSO to prepare mother solutions of various concentrations, and stored in a refrigerator. During the experiment, it was added to the cultured cells to prepare the desired final concentration of the drug.

[0042] Cell culture: human liver cancer cell line Hep G 2 Inoculate in a sterile culture bottle, add an appropriate amount of DMEM culture solution, and incubate at 37°C in 5% CO 2 cultured in an incubator with saturated humidity. The cells grow adherently in a monolayer and are passaged every 2-3 days. Digest and culture the cells with 0.25% trypsin + 0.02% EDTA for 2-4 minut...

Embodiment 2

[0047] Example 2: Detection of apoptosis

[0048] Hep G in logarithmic growth phase 2 cells, made into a single cell suspension, at 1×10 5 / ml inoculated in a 6-well plate, 2 ml per well, after the cells adhered to the wall, different concentrations of drugs were added, and the cells were collected after continuing to culture for 24 hours. Transfer to a centrifuge tube, centrifuge at 1000 r / min for 3 min, discard the supernatant, wash with incubation buffer solution, add 100 ml of labeling solution and disperse the cells with a 400-mesh nylon mesh, incubate at room temperature for 15 min in the dark, centrifuge at 800 rpm for 5 min, and rinse with incubation buffer After washing, add Annexin V / PI staining solution and incubate at 4°C for 20min, and then detect on the machine. The excitation light wavelength of the flow cytometer is 488 nm, and a filter with a wavelength of 515 nm is used to detect FITC fluorescence, and another filter with a wavelength greater than 560 nm is...

Embodiment 3

[0050] Embodiment 3: AO / EB fluorescent staining

[0051] Take the cells in the logarithmic growth phase, make a single cell suspension according to the subculture method, and count them at 1 × 10 5 / ml density in six-well plates. Place the culture plate at 37°C, 5% CO 2 After 24 hours of adherent culture in the incubator, add different concentrations of drugs to each well, and continue to incubate for 24 hours; collect all the cells, transfer them to a centrifuge tube, centrifuge at 1000 rpm for 3 minutes, wash twice with PBS, and add 2ml PBS to resuspend the cells , take 100 ml of cell suspension into EP tube, add 8 ml of AO / EB staining solution, and incubate for 15 min. Under the microscope, green light can stimulate EB to observe late apoptosis, and blue light can stimulate AO to observe early apoptosis.

[0052] The results showed that under the microscope the cells in the combined drug group were significantly shrunken and rounded, with blurred shape, chromatin condens...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a combined drug, in particular to a combined drug with anti-tumor activity. The drug comprises elemene substances with anti-tumor effect, nonsteroidal substances with anti-inflammatory effect and one or more medicinal carriers. Such synergic combination ensures the combined drug to have better anti-tumor activity.

Description

technical field [0001] The invention relates to a composition or compound product of elemene substances and non-sterol substances with anti-inflammatory effects, and their application in antitumor drugs. Background technique [0002] Cancer is a common and frequently-occurring disease that seriously threatens human life. At present, there are about 10 million new cancer patients and nearly 6 million cancer deaths in the world every year. In my country, there are about 1.8 million new cancer patients and 1.3 million deaths each year. And the incidence rate is increasing year by year. In my country, cancer death accounts for the second cause of death of various diseases, and it has already occupied the first place in the city. Cancer not only causes huge physical damage to patients, but also brings serious trauma to patients psychologically and spiritually. It has brought hardships to the lives of countless people, ruthlessly claimed countless lives and destroyed The happy an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K45/00A61K31/015A61K31/035A61K31/11A61K31/045A61P35/00
Inventor 绪广林吴昊杜逸芳蔡婕敖桂珍孙婷
Owner NANJING NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com