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Method for improving vitamin D content in lucid ganoderma calcium fortifier by ultraviolet irradiation

A Ganoderma lucidum calcium and fortifier technology, applied in botany equipment and methods, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problem of lack of bioidentity and balance, and the content cannot meet the needs of vitamin D, etc. question

Inactive Publication Date: 2010-12-08
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although edible and medicinal fungi are an important source of vitamin D in natural foods, their content is far from meeting the human body's demand for vitamin D
Vitamin D in calcium fortifiers currently on the market is also artificially added to assist calcium absorption, which does not have bioidentity and balance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Strain used: Ganoderma lucidum strain of CGMCC NO.5.535

[0082] A, prepare liquid culture medium: add following composition (weight / volume percentage) in every 100ml culture medium:

[0083] Sucrose 3.0g

[0084] Corn flour 3.0g

[0085] Soybean powder 1.5g

[0086] Yeast powder 0.1g

[0087] K H 2 PO 4 0.05g

[0088] MgSO 4 0.02g

[0089] FeSO 4 Add iron 0.01g

[0090] ZnSO 4 Add zinc 0.01g

[0091] Take Na 2 SeO 3 Added Selenium 0.00002g

[0092] to CrCl 3 Add chromium 0.00002g

[0093] CaCO 3 Add calcium 0.2g

[0094] VB 1 0.002g

[0095] Add water to dissolve and make the volume to 100ml;

[0096] Sterilize at 120°C for 30 minutes and then cool to 30°C;

[0097] b. Inoculate the ganoderma lucidum (Ganoderma lucidum) bacterial strain with the preservation number CGMCC NO.5.535 in the culture medium of the above-mentioned step a, which is preserved by the China Committee for the Preservation...

Embodiment 2

[0129] Strain used: Ganoderma lucidum strain of CGMCC NO.5.535.

[0130] A, prepare liquid culture medium: add following composition (weight / volume percentage) in every 100ml culture medium:

[0131] Sucrose 4.0g

[0132] Corn flour 4.0g

[0133] Soybean powder 2.0g

[0134] Yeast powder 0.2g

[0135] K H 2 PO 4 0.1g

[0136] MgSO 4 0.02g

[0137] FeSO 4 Add iron 0.01g

[0138] ZnSO 4 Add zinc 0.01g

[0139] Take Na 2 SeO 3 Added Selenium 0.00002g

[0140] to CrCl 3 Add chromium 0.00002g

[0141] with CaCl 2 Add calcium 0.3g

[0142] VB 1 0.003g

[0143] Add water to dissolve and make the volume to 100ml;

[0144] Sterilize at 120°C for 30 minutes and then cool to 30°C;

[0145] b. Inoculate the culture medium of the above step a into the Ganoderma lucidum strain preserved by the China Microorganism Culture Collection Management Committee, and the preservation number is CGMCC NO.5.535, and culture ...

Embodiment 3

[0176] Strain used: Ganoderma lucidum strain of CGMCC NO.5.535.

[0177] A, prepare liquid culture medium: add following composition (weight / volume percentage) in every 100ml culture medium:

[0178] Sucrose 6.0g

[0179] Corn flour 4.0g

[0180] Soybean powder 2.0g

[0181] Yeast powder 0.3g

[0182] K H 2 PO4 0.1g

[0183] MgSO 4 0.02g

[0184] FeSO 4 Add iron 0.01g

[0185] ZnSO 4 Add zinc 0.01g

[0186] Take Na 2 SeO 3 Added Selenium 0.00002g

[0187] to CrCl 3 Add chromium 0.00002g

[0188] With Ca(NO 3 ) 2 Added Calcium 0.4g

[0189] VB 1 0.003g

[0190] Add water to dissolve and make the volume to 100ml;

[0191] Sterilize at 120°C for 30 minutes and then cool to 30°C;

[0192] b. Inoculate the ganoderma lucidum (Ganoderma lucidum) bacterial strain with the preservation number CGMCC NO.5.535 in the culture medium of the above step a, which is preserved by the China Microorganism Culture Coll...

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Abstract

The invention discloses a method for improving vitamin D content in a lucid ganoderma calcium fortifier by ultraviolet irradiation. The method comprises the following steps of: a, preparing a culture medium, namely preparing a liquid culture medium from a carbon source, a nitrogen source, mineral elements and the like, and sterilizing and cooling the prepared culture medium; b, inoculating lucid ganoderma strains into the culture medium, and performing shaking culture under certain conditions so as to obtain liquid cordyceps sinensis strains; c, adding a certain amount of liquid lucid ganoderma strains into the culture medium, introducing gas, stirring for culture, and taking the fermentation liquor out; and d, selecting a UV-B ultraviolet light source with the power of 14 to 60W and the wavelength of 280 to 325nm, pouring the fermentation liquor of fungus lucid ganoderma into a large culture dish, putting the ultraviolet light source over the culture dish to irradiate the obtained fermentation liquor, concentrating and drying the irradiated fermentation liquor, and breaking walls and grinding into powdery products. The method for improving the vitamin D content has the advantages of simple process and convenient operation, and can be widely applied to the prevention and treatment of rickets in children, calcium deficiency in women and osteoporosis in old people.

Description

technical field [0001] The present invention relates to calcium-supplementing food and pharmaceutical products, and more specifically relates to a method for increasing the vitamin D content in Ganoderma lucidum calcium fortifier by ultraviolet light irradiation. Photoconversion of sterols to vitamin D2. It can be used in the preparation of medicines or health food for the prevention and treatment of calcium deficiency in children and women; the osteoporosis of the elderly and other diseases. Background technique [0002] A Chinese patent discloses a patent entitled "Preparation Method of Ganoderma Lucidum Bioactive Multidimensional Enhancer" (Application No. 03106293.8). The medicinal fungus Ganoderma lucidum is used as a biological carrier, and the inorganic calcium can be converted into active calcium in the chelated state of biological macromolecules through liquid fermentation. The product contains a variety of vitamins, bacterial immune proteins and bacterial polysac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A61K36/074A01G1/04A23L33/00
Inventor 胡征
Owner HUBEI UNIV OF TECH
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