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Method for preparing enhanced liquid microbial organic fertilizer

An organic fertilizer and microbial technology, applied in the field of liquid fertilizer preparation, can solve the problems of ambiguity and unstable efficacy of liquid fertilizer, and achieve the effect of clear function, easy operation and soil quality improvement.

Active Publication Date: 2010-11-03
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] The purpose of the present invention is to provide a method for intermittent aerobic fermentation by inoculating microorganisms with clear functions to prepare liquid microbial organic fertilizers for the problem of unstable and unclear efficacy of liquid fertilizers prepared from compost products

Method used

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  • Method for preparing enhanced liquid microbial organic fertilizer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Obtaining and strain characteristics of endophytic Bacillus subtilis strains:

[0024] The endophytic Bacillus subtilis bacteria described in this patent are obtained by the Institute of Agricultural Resources and Environment of the Jiangsu Academy of Agricultural Sciences from pepper plants grown in greenhouses, and are obtained by the method of isolating and screening endophytic bacteria. The bacteria can secrete a variety of antibacterial substances in a liquid culture state, such as antibacterial proteins, small molecule antibiotics, etc.; and the secreted small molecule antibiotics are still biologically active after being treated at 120°C for 30 minutes, and have excellent temperature stability. The bacterium has a strong effect on the production of more than ten kinds of pathogenic fungi such as common Fusarium wilt, Phytophthora, Blight, Verticillium dahliae, Sclerotinia, Botrytis cinerea and pathogenic bacteria such as bacterial wilt, canker, clubroot The growt...

Embodiment 2

[0028] Preparation of Bacillus subtilis culture medium

[0029] Preparation of Bacillus subtilis test tube slant medium: Weigh 0.3 grams of beef extract, 1.0 grams of peptone, and 0.5 grams of sodium chloride, dissolve them in 1000 ml of water, adjust the pH to 7.4-7.6, add 15-20 grams of agar, heat and boil until the agar Melt, make up the volume to 1000ml, cool down properly and divide into test tubes, the filling volume is 1 / 5~1 / 6 of the test tube height, plug the test tube mouth with a test tube stopper, autoclave at 121°C for 25 minutes, take it out while it is hot Put the inclined plane until it is cooled, and the height of the inclined plane shall not exceed 1 / 3 of the height of the test tube.

[0030] Preparation of liquid medium: 0.3 grams of beef extract, 1.0 peptone, 0.5 grams of sodium chloride, dissolved in 1000 ml of water, adjusted to pH 7.4 to 7.6, dissolved in 1000 ml of water, adjusted to pH 7.4 to 7.6, then divided into liquids The volume is 80-100ml / 250ml ...

Embodiment 3

[0033] Preparation of endophytic Bacillus subtilis powder

[0034] Put the preserved bacterial classification of the endophytic Bacillus subtilis of embodiment 1 into the test tube slant culture medium and carry out after the test tube slant plane activation, then put into the triangular flask that the capacity that liquid culture medium is housed is 250ml, and the filling volume is 80ml / 250ml ; Place the flask in a shaking table for 3-4 days, the rotating speed of the shaking table is 160r / min, and the ambient temperature during the cultivation period is 28 ± 2°C; after the end, insert the solid medium with 5% inoculum (V / W) Continue to cultivate in an environment of 28±2°C for 6-8 days, and stir once every 2-3 days until spores are produced; finally, air-dry naturally or dry at 30°C to obtain a solid bacterial agent, and the bacterial content in the solid bacterial agent for 10 10-11 cfu / g. After the solid microbial agent is crushed through a 20-40 mesh sieve, endophytic B...

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Abstract

The invention relates to a method for preparing an enhanced liquid microbial organic fertilizer. The method comprises the following steps of: mixing rotten compost products and water in a volume ratio of 1:8-10 in an opened barrel container; inoculating microbial agents; fermenting the mixture under an intermittent aerobic environment at the room temperature of between 20 and 25 DEG C; and filtering the mixture with a 100-mesh screen to obtain the organic fertilizer. The method has the advantages that: the method takes the rotten compost products such as cow dung, chicken dung and rice strawsas raw materials; and the obtained liquid fertilizer contains mineral elements such as quick-acting and slow-acting carbon, nitrogen, phosphorus and potassium elements which are necessary to crop growth, and a great number of aerobic microorganisms and metabolic products thereof. In the process of liquid fermentation, endogenous bacillus subtilis bacteria which can be colonized in plants are inoculated, so that the organic fertilizer has the advantages of more specific functions, stronger target property and more stable effect. The organic fertilizer also has the advantages of simple fermentation process equipment, simple fermentation process, easy operation, capabilities of improving soil quality, balancing soil nutrients and enriching population structures of soil microorganisms after application, and effects of promoting growth and preventing diseases for crops.

Description

technical field [0001] The invention relates to a preparation method of liquid fertilizer. Specifically, compost is made from a mixture of cow dung, chicken manure and rice straw, and then the compost product is used as a raw material, and inoculated with microorganisms that can colonize plants and have growth inhibitory effects on plant pathogens, and are prepared by secondary liquid fermentation The invention relates to a liquid fertilizer capable of preventing diseases and promoting the growth of crops, which belongs to the field of comprehensive utilization of agricultural waste. Background technique [0002] According to statistics, the annual production of agricultural waste in my country is about 3.8 billion tons, of which 1.75 billion tons are animal excrement, 650 million tons are crop straw, and 500 million tons are other waste. The speed continues to increase. The utilization of these wastes is currently mainly to produce solid organic fertilizers through compost...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F17/00C05F5/00C05G3/00C05G3/04C05G3/80
CPCY02A40/20Y02W30/40
Inventor 马艳常志州李艳霞黄红英徐跃定张建英
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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