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Microfluidic chip for rapid detection of saxitoxin and method for preparing same

A microfluidic chip and saxitoxin technology are applied in the preparation of test samples, measurement devices, biological tests, etc., and can solve problems such as environment and large volume that are not suitable for freshwater lakes

Inactive Publication Date: 2010-09-22
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the equipment is bulky and not suitable for the environment of freshwater lakes

Method used

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  • Microfluidic chip for rapid detection of saxitoxin and method for preparing same
  • Microfluidic chip for rapid detection of saxitoxin and method for preparing same
  • Microfluidic chip for rapid detection of saxitoxin and method for preparing same

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Embodiment Construction

[0040] Substrate preparation. Put the silicon chip into Piranha solution (98% concentrated sulfuric acid: 30% hydrogen peroxide = 7: 3) and boil for 15 min. Rinse five times with deionized water, blow dry with nitrogen, and bake at 200 °C for 30 min.

[0041] Spray paint. Pour Microchem's SU-8 glue (the same below) on the center of the silicon wafer, hold the edge of the silicon wafer with your hand, tilt it and rotate it slowly, so that SU-8 covers most of the silicon wafer, and let it stand for 15 minutes. Spin coating with a spin coater (Spin-CoaterKW-4A, Chemat Technology, Inc.): Spin coating at 3000 rpm for 60 s to make the glue more evenly distributed, and let stand for 10 min to alleviate the edge protrusion effect.

[0042] Soft bake. The purpose of soft baking is to volatilize the solvent in the SU-8 photoresist. During soft drying, the solvent is evaporated at a controlled rate. Keep at 65°C and 95°C for 3min and 6min, respectively. Then slowly lower to room te...

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Abstract

The invention belongs to the technical field of biologic analysis and detection, and in particular relates to a microfluidic chip for rapid detection of saxitoxin and a method for preparing the same. The chip is made of the material such as optically transparent dimethyl silicone polymer by the molding method and mainly comprises a sample reaction micro-channel layer, a valve control layer and a substrate layer. The chip comprises a sample enrichment and immunity analysis module and a signal acquisition module, wherein each module consists of a plurality of paralleled immunity chromatographiccolumn micro analysis chambers of nanoliter volume; and saxitoxin antibody protein or antigen is fixed in each analysis chamber so as to realize rapid on-site detection of the saxitoxin in the sample. The chip can perform automatic signal acquisition, remote transmission and signal analysis due to the characteristics of high speed, high efficiency, convenient carrying, low cost and easy automation control and is suitable for on-site rapid detection of the saxitoxin in the water environment and remote detection in a large range.

Description

technical field [0001] The invention belongs to the technical field of biological analysis, detection and control, and in particular relates to a microfluidic chip for on-site rapid detection of the paralytic shellfish poison saxitoxin, and provides a preparation method of the chip. Background technique [0002] Red tides have a serious impact on the marine environment, marine fisheries, aquaculture, tourism and human health. When a red tide breaks out, it will destroy the aquatic ecology and lead to the death of a large number of aquatic organisms. Some algae in red tides contain various marine toxins, which can directly poison fish, shrimp, and shellfish, seriously affecting mariculture and fishery. What's more serious is that these toxins can also be enriched in fish, shrimp and shellfish, which can cause poisoning after being eaten by humans, and in severe cases lead to death. Sea water is also irritating to human skin during red tide. At the same time, it also damage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/544G01N33/552G01N33/52G01N1/28
Inventor 隋国栋刘思秀张金玲赵望刘超
Owner FUDAN UNIV
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