Method for evaluating efficacy of antler

A deer antler and drug efficacy technology is applied in the field of evaluating the influence of deer antler on the growth and metabolism of characteristic microbial flora in the gastrointestinal tract, which can solve the problems of difficulty in comprehensively characterizing characteristic information, inability to obtain dynamic data, and easy loss of overall information, so as to be beneficial to accurate determination. , conducive to standardization, easy to standardize the effect

Inactive Publication Date: 2012-08-29
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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Problems solved by technology

[0004] Traditional research methods such as cellular immune reaction detection, immunohistochemistry, hemodynamics, and erythrocyte agglutination have been used to evaluate the active parts of velvet antler, but these methods often have single evaluation indicators, easy loss of overall information, and interference factors. It is difficult to fully characterize the characteristic information of the body in the process of drug intervention, and it is impossible to obtain real-time dynamic data.

Method used

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  • Method for evaluating efficacy of antler
  • Method for evaluating efficacy of antler
  • Method for evaluating efficacy of antler

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Effects of each extraction part of velvet antler alcoholic extract on the growth and metabolism of beneficial bacteria in the gastrointestinal tract

[0053] 1. Preparation of the test solution

[0054] Take 12g of sika deer antler powder, add 10 times the amount of 80% ethanol to reflux extraction for 3 times, each time for 1 hour, combine the medicinal liquid, concentrate it into an extract with a relative density of 0.9g / ml; adopt the system solvent extraction method, use ether, chloroform , ethyl acetate and n-butanol to the fractional extraction of the extract, each solvent is extracted 3 times, each 20ml, and each extract is combined respectively to obtain the ether part, the chloroform part, the ethyl acetate part and the n-butanol part. Each extraction part was dried under reduced pressure to obtain a dry paste; respectively take 0.2 g of each extraction part dry paste, add an appropriate amount of sterile water to dissolve, and prepare a concentration...

Embodiment 2

[0072] Example 2 The effect of the n-butanol extraction part of velvet antler alcoholic extract on the growth and metabolism of harmful bacteria in the gastrointestinal tract

[0073] 1. Measurement method

[0074] 1×10 6 Staphylococcus aureus was inoculated into peptone medium to form a suspension of Staphylococcus aureus at a concentration of 1.5×10 6 Cell / mL; Get the suspension 0.05ml of Staphylococcus aureus, mix in the ampoule bottle with the need testing solution 1.95ml of the velvet antler n-butanol extraction part different concentration of preparation in embodiment 1, seal, use ampoule lifter Put the ampoule into the preheating position on the upper half of the measuring cylinder, preheat the sample for 20 minutes, then drop the sample into the measuring cup for detection, and record the thermal spectrum curve; when the curve returns to the baseline, the experiment ends. The thermal spectrum curve of Staphylococcus aureus growth and metabolism under the action of n-...

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Abstract

The invention relates to a method for evaluating efficacy of an antler, which takes the bifidobacterium in a gastrointestinal tract as representative of beneficial bacterium and takes staphylococcus as representative of harmful bacterium.The method objectively and quantitatively evaluates the influence of different extraction sites of antler alcohol extract on the growth and metabolism of microorganism in the gastrointestinal tract through obtaining a thermogram and other comprehensive information which can fully reflect the interaction process and has overall expression characteristics, screens the active site with the strongest bacterium-promoting action of the antler and inspects the bacteriostasis of the active side simultaneously. The method has simple operation, easy standardizationand automation of the detection.

Description

technical field [0001] The invention relates to a method for evaluating the efficacy of velvet antler, in particular to a method for evaluating the effect of velvet antler on the growth and metabolism of characteristic flora in the gastrointestinal tract. Background technique [0002] Deer antler is the unossified and densely haired young horns of the deer Cervus nippon Temminck or the red deer Cervuselaphus Linnaeus, which has the functions of promoting growth and metabolism, strengthening kidney and yang, and strengthening bones and muscles. With the deepening of research, people have extracted and isolated more and more chemical components from different active parts of velvet antler, and there are many research reports on the pharmacological effects of velvet antler. affect its promotion and application. Therefore, objective and sensitive characterization of the efficacy of velvet antler is of great significance for the quality standard control of velvet antler and effe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02G01N25/20
Inventor 鄢丹肖小河韩玉梅张萍唐慧英
Owner THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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