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Breeding process of high-titer strains of streptomyces avermitilis strains subjected to heavy ion irradiation mutagenesis

A Streptomyces avermanii and ion irradiation technology, applied in the field of antibiotic breeding, can solve the problems such as difficult conditions to achieve, and achieve the effect of uniform pH value and accurate nutrients

Inactive Publication Date: 2010-07-07
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But conditions are limited and difficult to achieve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0064] 1. Preparation medium

[0065] 1. Fermentation bottle culture medium

[0066] (1) First measure 600ml of tap water and pour it into No. 1 beaker to boil, weigh 15g of starch and beat it into a paste, pour the starch into boiling water to fully gelatinize;

[0067] (2) Accurately weigh 0.01g of cobalt chloride with a ten-thousandth balance, add it to No. 2 beaker, add 300ml of water, stir and dissolve on a magnetic stirrer, and weigh 12g of bean cake powder and 8g of peanut cake powder after cobalt chloride is fully dissolved , 6g of yeast powder and 10g of dextrin were added to No. 2 beaker, and after being fully mixed, they were transferred to No. 1 beaker;

[0068] (3) Use No. 3 small beaker to accurately weigh 5g of yeast paste, add a small amount of water and stir to dissolve with a glass rod. After the dissolution is complete, add to No. 1 beaker, add water to 1000ml, and keep stirring with a glass rod to mix all the materials evenly;

[0069] (4) Use an acidity ...

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Abstract

The invention relates to a breeding process of high-titer strains of streptomyces avermitilis strains subjected to heavy ion irradiation mutagenesis, belonging to the field of antibiotic breeding. The breeding process further researches breeding to obtain an optimal breeding process after heavy ion irradiation and is characterized in that fermentation temperature is (28+ / -0.6) DEG C; the pH of a fermentation medium is 6.5-7.5; the rotation speed of a bottle rocker is 220-260 r / min; the inoculation age is 22-26 hours; the inoculation amount is 4-5 percent; and the fermentation medium comprises the following components: starch, dextrin, bean cake powder, peanut cake powder, yeast powder, yeast cream, cobalt chloride, bean oil and water. The invention has high fermentation titer of more than 6000 microgrammes / milliliter, reliable, controllable, adjustable and stable process, rich culture medium resources and low cost.

Description

technical field [0001] The invention relates to the field of antibiotic breeding, especially the breeding of Streptomyces avermanii. Background technique [0002] Abamectin is a metabolite extracted from Streptomyces avermanii after liquid fermentation, and is a sixteen-membered macrolide antibiotic. Its preparations can be used as pesticides and veterinary drugs, and have the characteristics of broad spectrum, high efficiency, low toxicity, no residue, and not easy to produce drug resistance. [0003] In terms of pesticides, abamectin is an agricultural antibiotic insecticide and acaricide, which is an insect nerve agent, which mainly interferes with the neurophysiological activities of pests, causing paralysis, poisoning and death. No systemic property, but strong osmotic effect, and can conduct laterally in plants, with high insecticidal and acaricidal activities, 5-50 times higher than commonly used pesticides, and the dosage is only 1%-2% of commonly used pesticides ....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01
Inventor 王曙阳陈积红李文建刘敬颉红梅
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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