Gene-activated poly-lactic acid material for treating liver diseases and preparation method thereof
A polylactic acid material and a technology of gene activation, which are applied in the field of gene activated polylactic acid material and its preparation, can solve the problems such as integration of polylactic acid implant and surrounding tissue of the liver, long-term stability, etc., and achieve good cell compatibility. , improve the transfection rate, low cost effect
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[0031] ①Preparation of galactosylated chitosan
[0032] Configure NaHCO 3 / acetic acid buffer solution (pH4.7 of the buffer solution, NaHCO in the solution 3 The concentration is 0.1N); Take lacturonic acid (LA) and dissolve it in NaHCO 3 / acetic acid buffer solution, prepared into a molar concentration of 64mM lacturonic acid solution, then added 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinyl Imine (NHS), in the resulting solution, the molar ratio of LA, NHS and EDC is 2:1:1, of course, the molar ratio of LA, NHS and EDC is 1:1:1-2: 1:1 is acceptable;
[0033]Stir magnetically at room temperature for 5 minutes to completely dissolve the material, then place it at 4°C for 3 hours, then react at room temperature for 3 hours, then add chitosan to the solution (the molar ratio of LA to chitosan is 2:1), and keep stirring For 3 days, dialyze in deionized water with a dialysis bag for 3 days, and obtain a galactose grafting rate of 10.9% galactosylat...
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