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Bacterial cellulose (BC) based antibacterial dry film applied to acute injury as well as preparation method and application thereof

A bacterial cellulose and acute wound technology is applied in the field of bacterial cellulose-based antibacterial dry film and its preparation, which can solve problems such as the bacterial cellulose-based antibacterial dry film that has not yet been seen, and achieves a simple and easy preparation method, good performance, Good breathability

Inactive Publication Date: 2010-06-23
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the research and development of bacterial cellulose-based antibacterial dry film for acute trauma at home and abroad.

Method used

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  • Bacterial cellulose (BC) based antibacterial dry film applied to acute injury as well as preparation method and application thereof
  • Bacterial cellulose (BC) based antibacterial dry film applied to acute injury as well as preparation method and application thereof
  • Bacterial cellulose (BC) based antibacterial dry film applied to acute injury as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Preparation and treatment of BC membrane

[0036] Acetobacter xylinum (Acetobacter xylinum) was expanded and cultivated in liquid seed medium at 30°C for 24 hours according to the inoculation amount of 2 to 3 inoculation loops, and then transferred to the fermentation medium at 30°C for static culture at 6% of the inoculum amount. Day, harvest bacterial cellulose (BC) wet film; The prepared BC film is immersed in 1% NaOH solution in a water bath at 80°C until the BC film becomes white and translucent, and then the prepared BC film is cut into 15mm diameter After being freeze-dried by a freeze-drying apparatus, the discs were weighed for dry weight and set aside;

[0037] Among them, the components of the seed medium and the fermentation medium are: mannitol, glucose, sucrose or fructose 25g, peptone or tryptone 3g, yeast extract 5g, water 1L, pH 5.0, sterilized at 121°C for 20min;

[0038] (2) Preparation of antibacterial agent solution

[0039] Preparation of the...

Embodiment 2

[0047] (1) Preparation and treatment of BC membrane

[0048] Gluconacetobacter xylinum (Gluconacetobacter xylinum) was expanded in the liquid seed medium at 30°C for 24 hours according to the inoculation amount of 2 to 3 inoculation loops, and then transferred to the fermentation medium at 25°C with a 10% inoculum amount. Place and cultivate for 10 days, harvest bacterial cellulose (BC) wet film; the prepared BC film is immersed in 1.5% NaOH solution in a water bath at 90°C until the BC film becomes white and translucent, and then the prepared BC film is cut into diameter It is a 15mm disc, which is lyophilized by a lyophilizer for subsequent use;

[0049] Among them, the components of the seed medium and the fermentation medium are: mannitol, glucose, sucrose or fructose 20g, yeast extract 5g, peptone or tryptone 5g, citric acid 1.15g, Na 2 HPO 4 2.7g, 1L of water, pH7.0, sterilized at 121°C for 20min;

[0050] (2) Preparation of antibacterial agent solution

[0051] Pre...

Embodiment 3

[0065] (1) Preparation and treatment of BC membrane

[0066] Gluconobacter oxydans (Gluconobacter oxydans) was expanded and cultivated in liquid seed medium at 30°C for 24 hours according to the inoculum amount of 2 to 3 inoculation loops, and then transferred to the fermentation medium at 15% inoculum amount for static culture at 20°C for 10 Day, harvest bacterial cellulose (BC) wet film; The prepared BC film is immersed in 1% NaOH solution in a water bath at 80°C until the BC film becomes white and translucent, and then the prepared BC film is cut into 15mm diameter Discs, freeze-dried by a freeze-drying apparatus for later use;

[0067] Among them, the components of the seed medium and the fermentation medium are: mannitol, glucose, sucrose or fructose 25g, peptone or tryptone 3g, yeast extract 5g, water 1L, pH 4.0, sterilized at 121°C for 20min;

[0068] (2) Preparation of antibacterial agent solution

[0069] Preparation of domiphene solution: draw 5.9-105 μL of 85% dom...

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Abstract

The invention relates to a bacterial cellulose (BC) based antibacterial dry film applied to acute injury, comprising the following components: a BC dry film and an antibacterial agent, wherein the drug loading rate of the BC based antibacterial dry film is 0.0114-0.2027mg / cm2. A preparation method of the BC based antibacterial dry film comprises the steps of: after implanting production strains of the BC into a seed culture medium for propagation culture, transplanting the strain to a fermentation medium for static culture, obtaining a BC wet film for soaking and water-bath, cutting the wet film into sheets for drying, dipping the sheets in a prepared antibacterial agent solution for the secondary drying to obtain the BC based antibacterial dry film. In the invention, the BC based antibacterial dry film has the advantages of light and soft texture, good biocompatibility, high hygroscopicity, good air permeability, durable antibiotic property and the like; in addition, the preparation method is simple, convenient and easy in operation and has low cost; in the practical application process, the BC based antibacterial dry film can be sheared into any shapes, is not adhered, does not irritate to skin, can be rapidly degraded in the environment after being abandoned, and can be taken as a green and environment-friendly functional antibacterial dressing.

Description

technical field [0001] The invention belongs to the field of bacterial cellulose-based antibacterial film and its preparation method and application, in particular to a bacterial cellulose-based antibacterial dry film for acute wounds, its preparation method and application. Background technique [0002] Bacterial Cellulose (BC) is a kind of natural cellulose synthesized by microorganisms. From the perspective of chemical molecular formula, BC is no different from ordinary plant cellulose, but it has extremely high mechanical properties due to its unique physical and chemical structure. strength and biocompatibility. The formed BC is composed of ribbon-shaped microfibers, which are highly crystalline and highly single-oriented fibers, which are intricately entangled together to form a unique three-dimensional network structure. There are many "holes" inside the unique three-dimensional network structure of BC, which has good air permeability, Water permeability, can absorb ...

Claims

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Application Information

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IPC IPC(8): A61L15/28A61L15/44C12P19/04C12R1/01C12R1/02C12R1/025C12R1/05C12R1/065C12R1/38C12R1/41
Inventor 洪枫魏斌杨光杨雪霞
Owner DONGHUA UNIV
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