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Biological method for extracting grease from flat almond

A technology for oil extraction and biology, applied in the direction of oil/fat production, fat production, fermentation, etc., can solve the problems of low extraction efficiency, increased cost, and limited industrial application

Inactive Publication Date: 2010-06-16
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to supercritical CO 2 The extraction pressure is high, the extraction efficiency is low, and the number of cycles is long, which increases the cost and limits its large-scale industrial application

Method used

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  • Biological method for extracting grease from flat almond
  • Biological method for extracting grease from flat almond

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Such as figure 1 As shown, the flat almonds are crushed by a dry method with a pulverizer, the particle size is between 10-150 mesh, preferably 10-60 mesh, and the tissue cells of the flat almond raw material are destroyed. Mix the crushed flat almond powder and water in a mass ratio of 1:4, adjust the pH to 6.5, and the temperature to 50°C, add 0.1% neutral protease in the quality of the flat almond powder, and stir for 2 hours for enzymatic hydrolysis; then cool down to 40°C, then adjust the pH to 7.5, add 0.1% alkaline protease in the mass of flat almond powder, and then stir for 2 hours to obtain the enzymolysis solution; centrifuge the enzymolysis solution at 3500-4000r / min for 10min, To obtain free oil, emulsion, hydrolyzate and almond residue, add lipopeptide biological demulsifier accounting for 0.1% of the emulsion volume to the obtained emulsion, and after demulsification, centrifuge again at 3500-4000r / min for 10min to obtain free oil Combine the free oil ob...

Embodiment 2

[0020] Such as figure 1 As shown, the flat almonds are crushed by a dry method with a pulverizer, the particle size is between 10-150 mesh, preferably 10-60 mesh, and the tissue cells of the flat almond raw material are destroyed. Mix the crushed flat almonds and water with a mass ratio of 1:8, adjust the pH to 7.0, and the temperature is 50°C, add 0.5% neutral protease of the quality of flat almond powder, stir for 4 hours; then cool down to 40°C, adjust the pH to 8.0, add alkaline protease with 0.5% mass of flat almond powder, and then stir for 3 hours to obtain the enzymolysis solution; centrifuge the enzymolysis solution at 3500-4000r / min for 10min, then top-down Obtain free oil, emulsion, hydrolyzate and almond residue, add lipopeptide biological demulsifier accounting for 0.3% of the emulsion volume to the obtained emulsion, after demulsification, centrifuge again at 3500-4000r / min for 10min to obtain free oil; combine The free oil obtained by secondary centrifugation c...

Embodiment 3

[0022] Such as figure 1 As shown, the flat almonds are crushed by a dry method with a pulverizer, the particle size is between 10-150 mesh, preferably 10-60 mesh, and the tissue cells of the flat almond raw material are destroyed. Mix the crushed flat almonds and water with a mass ratio of 1:10, adjust the pH to 7.5, and the temperature is 50°C, add 1.0% of the mass of flat almond powder with neutral protease, and perform enzymatic hydrolysis for 6 hours; then lower the temperature to 40 ℃, adjust the pH to 8.5, add almond powder mass 1.0% alkaline protease, and then stir for 6 hours for enzymolysis to obtain an enzymolysis solution. After centrifuging the enzymatic solution at 3500-4000r / min for 10 minutes, free oil, emulsion, hydrolyzate and almond residue are obtained from top to bottom, and the obtained emulsion is added with a lipopeptide biodemulsifier accounting for 0.5% of the emulsion volume, After demulsification, centrifuge again at 3500-4000r / min for 10 minutes to...

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Abstract

The invention relates to a biological method for extracting grease from flat almonds. The method comprises the following steps of: crushing the flat almonds in a dry way, mixing the crushed flat almond powder and water, regulating pH to 6.5-7.5 and the temperature to 50 DEG C, adding neutral proteinase accounting for 0.1-1.0% of the flat almond powder to carry out enzymolysis for 2-6 hours, reducing the temperature to 40 DEG C and regulating the pH 7.5-8.5, adding alkaline proteinase accounting for 0.1-1.0% of the raw materials to carry out enzymolysis for 2-6 hours to obtain enzymolysis liquid; directly centrifuging the enzymolysis liquid to respectively obtain free oil, emulsion, hydrolytic liquid and almond dregs from top to bottom, adding biological lipopeptid demulsifying agents accounting for 0.1-0.5% of the volume of emulsion into the emulsion, demulsifying and centrifuging again for separation to obtain free oil, and merging the free oil to obtain flat almond oil. The invention can avoid the formation of stable emulsion by adopting a dry method, the demulsifying agents are saved, and the cost is reduced; the flat almond oil prepared by the biological method can avoid problems of fat oxygenation caused by squeezing grease, and solvent remain caused by extracting oil in a lixiviation method.

Description

technical field [0001] The invention belongs to oil extraction technology, in particular to a biological method for extracting oil from flat almonds. Background technique [0002] Apricot is a plant of the genus Apricot in the Rosaceae family. It is mainly distributed in Northeast my country and North China. Due to its cold resistance, drought resistance, barren resistance, and easy management, it has gradually become one of the main economic forest tree species in the "Three Norths" region. It is rich in nutritional value, containing 25% protein and about 50% crude fat. Sweet almond oil contains a large amount of unsaturated fatty acids, mainly oleic acid and linoleic acid, accounting for more than 90% of the total fatty acids. It is a good health-care edible oil. In addition, almond oil remains clear at -10°C and does not condense at -20°C, so it can be used as lubricating oil for precision instruments and base oil for cosmetics. At present, supercritical CO has been use...

Claims

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Application Information

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IPC IPC(8): C12P7/64C11B1/00C11B1/04
Inventor 钱建华何余堂刘贺朱丹实
Owner BOHAI UNIV
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