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Method for reducing heavy metal ions in oyster hydrolysis liquid by using crab shell meal

A technology of heavy metal ions and hydrolyzate, which is applied in the fields of application, food preparation, food science, etc., can solve the problems of strict breeding environment requirements, difficult removal of heavy metals, and harm to human health, and achieve the goal of reducing pollution, low cost, and sufficient resources Effect

Inactive Publication Date: 2010-06-16
DALIAN FISHERIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because heavy metals are not easy to remove and easy to produce enrichment effects, if people eat them frequently, heavy metal poisoning will occur, which will seriously endanger their health.
At present, the heavy metal removal methods for oysters and other shellfish products mainly focus on the quality control during the breeding process, focusing on the removal of live bodies, but this method has strict requirements on the breeding environment and high farming costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] The oyster hydrolyzate can be prepared according to the Chinese patent application No. 200510047583.4, entitled "Preparation Method of Oyster Antioxidant Peptide Health Products". Wash the crab shells with deionized water, dry at 60°C, crush and sieve to prepare crab shell powder with a particle size of 60 mesh, adjust the pH value of the oyster hydrolyzate to 2 with 0.1N HCl, add 0.5g / L crab shell powder , shaken in a water bath at 10°C for 0.5h, centrifuged and filtered to get the supernatant. The mixed acid (4:1) of nitric acid and perchloric acid was used to digest the oyster hydrolyzate before and after treatment to obtain a clear and transparent solution. The content of cadmium, lead and chromium in the obtained clear and transparent solution was measured by atomic absorption spectrophotometer, and the content of amino acid nitrogen in the oyster hydrolyzate before and after treatment was measured by potentiometric titration. The results showed that the removal of...

Embodiment 2

[0011] Take 3kg of oysters, add 4.5kg of water and 10g of subtilisin after homogenization, adjust the pH value to 6.0 with 0.1N HCl and 0.1NNaOH, hydrolyze at 55°C for 120min and inactivate, pass the enzymatic solution through 100 mesh sieve and 330 mesh sieve respectively , and then carry out ultrafiltration to filtrate (filter membrane is 30000D), obtain oyster hydrolyzate. The crab shells were washed with deionized water, dried at 98° C., crushed and sieved to prepare crab shell powder with a particle size of 300 mesh. Use 0.1N NaOH to adjust the pH value of the hydrolyzate to 7, add 8g / L crab shell powder, shake in a water bath at 40°C for 6h, and centrifuge to obtain the supernatant. The mixed acid (4:1) of nitric acid and perchloric acid was used to digest the oyster hydrolyzate before and after treatment to obtain a clear and transparent solution. The content of cadmium, lead and chromium in the obtained clear and transparent solution was measured by atomic absorption ...

Embodiment 3

[0013] Take 3kg of oysters, add 4.5kg of water and 10g of papain after homogenization, adjust the pH to 6.0 with 0.1N HCl and 0.1N NaOH, hydrolyze at 55°C for 120min, and then inactivate. The enzymolysis solution is passed through a 100-mesh sieve and a 330-mesh sieve respectively, and then the filtrate is subjected to ultrafiltration (the filter membrane is 30000D) to obtain an oyster hydrolyzate. Wash the crab shells with deionized water, dry at 80°C, crush and sieve to prepare crab shell powder with a particle size of 150 mesh, adjust the pH value of the hydrolyzate to 6 with 0.1N NaOH, add 3g / L crab shell powder, and Shake in a water bath at 20°C for 3 hours, then centrifuge and filter to get the supernatant. The mixed acid (4:1) of nitric acid and perchloric acid was used to digest the oyster hydrolyzate before and after treatment to obtain a clear and transparent solution. The content of cadmium, lead and chromium in the obtained clear and transparent solution was deter...

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PUM

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Abstract

The invention discloses a method for reducing heavy metal ions in an oyster hydrolysis liquid by using crab shell meal, which comprises the following steps: washing a crab shell through de-ionized water, drying and crushing the crab shell; adding the obtained meal into the oyster hydrolysis liquid with an addition amount of 0.5 to 8 g / L, regulating pH of the hydrolysis liquid to between 2.0 and 7.0 through 0.1 percent NHCL or 0.1 percent NaOH; and oscillating the solution at the temperature of between 10 and 40 DEG C for 0.5 to 6h, centrifuging and filtering the solution, and obtaining supernatant. The method can effectively reduce the content of heavy metal cadmium, lead and chromium in the oyster hydrolysis liquid, does not influence the original taste and nutrition constituents of the hydrolysis liquid, and has the advantages of simple operation, low cost and high efficiency, and can realize large-scale industrial production.

Description

Technical field: [0001] The invention relates to the technical field of food and aquatic product processing, in particular to a method for reducing heavy metal ions in oyster hydrolyzate by using crab shell powder, which can fully utilize resources, reduce environmental pollution, and is simple to operate and low in cost. Background technique: [0002] Oyster is a high-quality marine cultured shellfish, containing 45% to 56% protein, 7% to 16% fat, 19% to 38% glycogen, and contains a variety of vitamins and niacin, carbohydrates and calcium, zinc, Nutrients such as iron and iodine. The high content of taurine in oysters is highly valued by the medical community. It is believed to be beneficial to the brain, reduce fat and lose weight, and promote the decomposition of cholesterol. At the same time, a large amount of glycogen contained in oysters is also used as human cells for metabolism. Therefore, a variety of oyster deep-processing products have been developed, such as oy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/015A23L1/327A23L1/33A23L5/20A23L17/20A23L17/40
Inventor 任丹丹汪秋宽侯亦舒范建军程晶吴超何云海
Owner DALIAN FISHERIES UNIVERSITY
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