Method for reducing heavy metal ions in oyster hydrolysis liquid by using crab shell meal
A technology of heavy metal ions and hydrolyzate, which is applied in the fields of application, food preparation, food science, etc., can solve the problems of strict breeding environment requirements, difficult removal of heavy metals, and harm to human health, and achieve the goal of reducing pollution, low cost, and sufficient resources Effect
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Embodiment 1
[0009] The oyster hydrolyzate can be prepared according to the Chinese patent application No. 200510047583.4, entitled "Preparation Method of Oyster Antioxidant Peptide Health Products". Wash the crab shells with deionized water, dry at 60°C, crush and sieve to prepare crab shell powder with a particle size of 60 mesh, adjust the pH value of the oyster hydrolyzate to 2 with 0.1N HCl, add 0.5g / L crab shell powder , shaken in a water bath at 10°C for 0.5h, centrifuged and filtered to get the supernatant. The mixed acid (4:1) of nitric acid and perchloric acid was used to digest the oyster hydrolyzate before and after treatment to obtain a clear and transparent solution. The content of cadmium, lead and chromium in the obtained clear and transparent solution was measured by atomic absorption spectrophotometer, and the content of amino acid nitrogen in the oyster hydrolyzate before and after treatment was measured by potentiometric titration. The results showed that the removal of...
Embodiment 2
[0011] Take 3kg of oysters, add 4.5kg of water and 10g of subtilisin after homogenization, adjust the pH value to 6.0 with 0.1N HCl and 0.1NNaOH, hydrolyze at 55°C for 120min and inactivate, pass the enzymatic solution through 100 mesh sieve and 330 mesh sieve respectively , and then carry out ultrafiltration to filtrate (filter membrane is 30000D), obtain oyster hydrolyzate. The crab shells were washed with deionized water, dried at 98° C., crushed and sieved to prepare crab shell powder with a particle size of 300 mesh. Use 0.1N NaOH to adjust the pH value of the hydrolyzate to 7, add 8g / L crab shell powder, shake in a water bath at 40°C for 6h, and centrifuge to obtain the supernatant. The mixed acid (4:1) of nitric acid and perchloric acid was used to digest the oyster hydrolyzate before and after treatment to obtain a clear and transparent solution. The content of cadmium, lead and chromium in the obtained clear and transparent solution was measured by atomic absorption ...
Embodiment 3
[0013] Take 3kg of oysters, add 4.5kg of water and 10g of papain after homogenization, adjust the pH to 6.0 with 0.1N HCl and 0.1N NaOH, hydrolyze at 55°C for 120min, and then inactivate. The enzymolysis solution is passed through a 100-mesh sieve and a 330-mesh sieve respectively, and then the filtrate is subjected to ultrafiltration (the filter membrane is 30000D) to obtain an oyster hydrolyzate. Wash the crab shells with deionized water, dry at 80°C, crush and sieve to prepare crab shell powder with a particle size of 150 mesh, adjust the pH value of the hydrolyzate to 6 with 0.1N NaOH, add 3g / L crab shell powder, and Shake in a water bath at 20°C for 3 hours, then centrifuge and filter to get the supernatant. The mixed acid (4:1) of nitric acid and perchloric acid was used to digest the oyster hydrolyzate before and after treatment to obtain a clear and transparent solution. The content of cadmium, lead and chromium in the obtained clear and transparent solution was deter...
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