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D-glucosamine-modified iron oxide nanoparticles and preparation method of lyophilized powder thereof

A technology of iron oxide nanoparticles and glucosamine, which is applied in the fields of emulsion delivery, MRI/MRI contrast agent, drug delivery, etc. It can solve the problems of not targeting tumors and affecting the application effect in the field of tumor targeting diagnosis, etc. Achieve a non-irritating effect on blood vessels

Inactive Publication Date: 2010-04-28
ZHENJIANG NO 1 PEOPLES HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although SPION has good superparamagnetism, it can be swallowed by phagocytes in the body and accumulate in the reticuloendothelial system, but it has no tumor-targeting properties, which affects its application in the field of tumor-targeted diagnosis.

Method used

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  • D-glucosamine-modified iron oxide nanoparticles and preparation method of lyophilized powder thereof
  • D-glucosamine-modified iron oxide nanoparticles and preparation method of lyophilized powder thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Add 0.1 mol of carbodiimide to 0.1 mol of citric acid-modified superparamagnetic iron oxide nanoparticles colloidal solution, mix well, and activate the carboxyl groups on the surface of iron oxide nanoparticles for 30 minutes at room temperature; add 0.1 mol of D-Glucosamine, mixed well, reacted with shaking at room temperature for 12 hours; dialyzed the above system with 50 times double distilled water for 10 hours; added 3% sorbitol to the system, heat sterilized, pre-frozen at -20°C for 12 hours, and freeze-dried for 10 hours. Hours, that's it.

Embodiment 2

[0021] Example 2: Add 0.5 mol carbodiimide and 0.1 mol N-hydroxysuccinimide to 0.1 mol dimercaptosuccinic acid-modified superparamagnetic iron oxide nanoparticle colloidal solution, mix well, and activate iron oxide nanoparticles at room temperature Carboxyl groups on the surface of grains for 60 minutes; add 0.2mol D-glucosamine to the above system, mix well, and react with shaking at room temperature for 24 hours; dialyze the above system with 100 times double distilled water for 12 hours; add 5% mannitol and 1% polyethylene glycol to the system Diol, sterilized by heating at 100°C for 30 minutes; pre-freezing at -40°C for 12 hours, then freeze-drying for 24 hours to obtain the product.

Embodiment 3

[0022] Example 3: Add 0.3 mol carbodiimide and 0.08 mol N-hydroxysuccinimide to 0.1 mol carboxy-terminal polyethylene glycol derivative modified superparamagnetic iron oxide nanoparticle colloidal solution, mix well, and activate at room temperature Carboxyl groups on the surface of iron oxide nanoparticles for 45 minutes; add 0.1mol D-glucosamine to the above system, mix well, and react with shaking at room temperature for 15 hours; dialyze the above system with 100 times double distilled water for 12 hours; add 5% glucose to the system, Sterilize with a 0.22 μm filter membrane; pre-freeze at -30°C for 10 hours, and then freeze-dry for 18 hours.

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Abstract

The invention relates to d-glucosamine-modified iron oxide nanoparticles used as a tracer for magnetic resonance, a preparation method of lyophilized powder thereof and use thereof. The d-glucosamine-modified iron oxide nanoparticles comprise superparamagnetic iron oxide nanoparticles, wherein the surfaces of the superparamagnetic iron oxide nanoparticles are modified by a substance having carboxyls; the carboxyls react with the aminos of the d-glucosamine to form amido bonds; and thus a structure that the surfaces of the superparamagnetic iron oxide nanoparticles are modified by the d-glucosamine is formed. The invention provides a novel tumor-targeted MRI contrast agent which has high selectivity of tumors for the tumor targeted magnetic resonance diagnostic tests and clinic tumor diagnosis. By preserving the d-glucosamine-modified superparamagnetic iron oxide nanoparticles in form of the lyophilized powder, the unstable problem of the d-glucosamine-modified superparamagnetic iron oxide nanoparticles is solved. The d-glucosamine-modified iron oxide nanoparticles are sterile, anhemolytic and free from vascular stimulation and can meet the requirements of tests of cells, animals and the like.

Description

technical field [0001] The invention relates to a preparation method of D-glucosamine modified iron oxide nanoparticles and freeze-dried powder which can be used as a magnetic resonance tracer in tumor diagnosis. Background technique [0002] Malignant tumors are the number one killer of human health. Early non-invasive diagnosis and targeted therapy of tumors have become the key to prolong survival and improve quality of life of cancer patients. Most malignant tumor cells have high metabolic characteristics, aerobic and anaerobic metabolism coexist, and tumor cells overexpress glucose transporter to absorb enough glucose. Therefore, glucose can be used as a targeting molecule for identifying tumor cells. At present, 18F-FDG (2-fluoro-18-fluoro-2-deoxy-D-glucose) has been used as a radioactive tracer, and the location, shape, size and number of tumors can be displayed by positron emission tomography (PET) and radioactivity distribution within the tumor. [0003] Superpar...

Claims

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Application Information

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IPC IPC(8): A61K49/08A61K49/18
Inventor 熊非顾宁单秀红朱夫蒋鹏程柳迎招
Owner ZHENJIANG NO 1 PEOPLES HOSPITAL
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