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Glaucescent fissistigma root saponin pharmaceutical composition and preparation method thereof

A technology of hibiscus and saponin, which is applied in the field of single-flavor traditional Chinese medicine extracts, can solve the problems of immature preparation technology and quality standards, unable to achieve large-scale production and preparation, and achieve large-scale industrial production and significant anti-tumor effects. , the effect of clear ingredients

Inactive Publication Date: 2009-12-30
CHIA TAI TIANQING PHARMA GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current means of separation of sagerin monomers is mainly the preparation of micro-sample in the laboratory, which can only be used for structural analysis and cannot reach the level of large-scale production preparation
[0007] Among the currently marketed Xiaoaiping preparations made from a single traditional Chinese medicine, the preparation process and quality standards are immature, and the active ingredients that reflect its clinical efficacy and pharmacological effects need to be further clarified

Method used

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  • Glaucescent fissistigma root saponin pharmaceutical composition and preparation method thereof
  • Glaucescent fissistigma root saponin pharmaceutical composition and preparation method thereof
  • Glaucescent fissistigma root saponin pharmaceutical composition and preparation method thereof

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Effect test

preparation example Construction

[0054] Preparation of the test solution: Accurately weigh 20.0 mg of Acacia saponin extract, dissolve it in methanol and transfer it to a 100 mL volumetric flask and set the volume to the mark as the test solution.

[0055] Preparation of the reference solution: Take 20.0 mg of each of Aegia saponins A, B, D, and H, weigh them accurately, add methanol to make a solution containing 200 μg per 1 ml, and obtain the solution.

[0056] Precisely draw 20 μL of the reference substance and the test solution, respectively, and inject them into the high-performance liquid chromatograph for determination.

Embodiment 1

[0058] Take 100kg of the medicinal material of Aegypti sinensis, crush and cut into coarse powder, put it into a heat reflux extraction tank, add 8 times the amount of 75% ethanol, heat and reflux for extraction 3 times, each time for 2 hours, release the extract, combine, filter, and concentrate the filtrate to 20L, add an equal volume of ethyl acetate to extract 3 times, combine the ethyl acetate solution, concentrate to a thick paste, then add 50L ethyl acetate to dissolve, filter, pass the filtrate through a neutral alumina column, and add 50L ethyl acetate Elution, TLC monitoring, collection of black bone saponin part, and concentrated to dryness, to obtain black bone saponin crude product (a) 3.2kg.

[0059] Take the crude product (a), add 100L of water, stir to dissolve, filter the plate and frame, pass the filtrate through a chromatographic column connected in series by polyamide (100-200 mesh, 20L)-macroporous resin (HPD-100, 100L), and wash thoroughly with water. Aft...

Embodiment 2

[0064] Take 100 kg of the medicinal material of Aegypti sinensis, crush and cut into coarse powder, put it into an extraction tank, add 10 times the amount of water, heat and reflux for extraction 3 times, each time for 2.5 hours, release the extract, combine, filter, and concentrate the filtrate to 50L, respectively Add an equal volume of ethyl acetate to extract 3 times, combine the ethyl acetate solution, concentrate to a thick paste, then add 50L ethyl acetate to heat to dissolve, filter, pass the filtrate through a neutral alumina column, and add 50L ethyl acetate to elute, TLC monitoring, collect the black bone saponin part, and concentrate to dryness to obtain 2.6kg of black bone saponin crude product (a).

[0065] Take the crude product (a), add 200L of water, stir to dissolve, filter the plate and frame, pass the filtrate through a chromatography column connected in series by polyamide (100-200 mesh, 40L)-macroporous resin (HPD-600, 150L), wash thoroughly with water A...

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Abstract

The invention relates to an extract of a single Chinese medicament, in particular to a glaucescent fissistigma root saponin extract and a preparation method thereof, a pharmaceutical composition, anti-tumor application thereof and the like.

Description

technical field [0001] The invention relates to an extract of a single traditional Chinese medicine, in particular, the invention relates to an extract of acacia saponin, its preparation method, application, pharmaceutical composition and the like. Background technique [0002] Black bone vine, also known as Tongguangsan, Tongguang vine, is the cane of Marsdenia tenacissima (Roxb.) Wight et Arn., a plant of the genus Marsdenia R.Br. in the family Asclepiadaceae. Mainly produced in Yunnan, Guizhou and other places, it was first recorded in "Southern Yunnan Materia Medica". Black bone vine contains various chemical components such as steroidal ester glycosides, alkaloids, organic acids, polysaccharides, resins and pigments. It has a wide range of pharmacological activities, such as anti-tumor, immune regulation, liver protection and diuretic effects. [0003] At present, Xiaoaiping, which is made from a single traditional Chinese medicine of Wuguteng, has a variety of dosage...

Claims

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Application Information

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IPC IPC(8): A61K36/27A61P35/00C07H1/08A61K31/704A61K135/00
Inventor 杨永安王衡奇石赟蓉郭安军王鸯张喜全徐宏江宋伟陈智林
Owner CHIA TAI TIANQING PHARMA GRP CO LTD
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