Agricultural composite microbial preparation and preparing method thereof
A technology of compound microbial bacterial agent and bacterial agent, applied in the direction of fertilization method, fertilizer mixture, application, etc., can solve problems affecting product quality, easy to infect miscellaneous bacteria, increase production cost, etc., to improve product quality, reduce production cost, The effect of shortening the fermentation time
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Embodiment 1
[0022] Example 1: Research on the fermentation process conditions of soybean rhizobia, Azotobacter chroococcus, Bacillus megaterium and Bacillus glaci according to the present invention
[0023] 1. Preparation of soybean rhizobia (ACCC15401 or ACCC15400 or ACCC15403) fermentation broth: the fermentation broth is sterilized at 121°C for 30 minutes, and the temperature is reduced to 32-33°C to inoculate the rhizobium seed solution, the inoculation amount is 1%-3%. During the fermentation process, the temperature is controlled at 32-34℃, and the aseptic air volume is controlled at 60-80m 3 / hour. Fermentation end time: 18-24 hours. The formula of fermentation broth: K 2 HPO 4 0.25-0.8g, NaCl 0.1-0.5g, MgSO 4 0.1-0.4g, mannitol 5-20g, yeast powder 0.4-1.5g, CaCO 3 1-5g, 1000mL water, pH 6.8-7. The preparation and sterilization process of seed culture is the same as fermentation culture.
[0024] 2. Preparation of Azotobacter chroococcus (ACCC10098) fermentation broth: the fermentation...
Embodiment 2
[0057] Example 2: Examples of the ratio of soybean rhizobia inoculum, Azotoxin-fixing bacteria inoculum, Bacillus megaterium inoculum and Bacillus coli inoculum of the present invention:
[0058] The ratio of the inoculum can be changed within the following range: the ratio of soybean rhizobia inoculum is 40-70% (weight percentage), the ratio of Azotoxin-fixing bacteria inoculum is 10-20% (weight percentage), and the ratio of Bacillus megaterium is 5-10% (weight percentage), and the proportion of Bacillus glaciens is 15-30% (weight percentage).
[0059] The proportioning method is: the bacteria liquid fermented according to the best fermentation conditions obtained in Example 1 is adsorbed on the adsorbent at a ratio of about 15-20% (weight percentage) (the ratio depends on the moisture content of the adsorbent) ), the adsorbent can use one or more of organic materials such as lignite, vermiculite, grass charcoal, bran, straw or animal manure. The product made of the adsorbent tha...
Embodiment 3
[0113] Example 3: Modification test of the compound microbial agent provided by the present invention on soil nutrients
[0114] Guangshun Chengpeng Orchard is located in Beijing. The soil quality in the garden is relatively poor, especially the content of organic matter is very low. Since 2003, the compound microbial inoculum provided by the present invention has been used (50% soybean rhizobia, Azotobacter chroococcus 20%, Bacillus megaterium 10%, and Bacillus glaciens 20% in proportions). The base fertilizer is applied or used as topdressing, and the ditch is applied to the soil at the root of the crop. In the same year, the orchard began to collect soil samples to analyze the indicators of soil organic matter, total nitrogen, available nitrogen, available phosphorus and available potassium. The results are summarized as follows:
[0115] Table 15: Test results of soil composition
[0116]
[0117] Note: The increase refers to the comparison of the test results in each year w...
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