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Agricultural composite microbial preparation and preparing method thereof

A technology of compound microbial bacterial agent and bacterial agent, applied in the direction of fertilization method, fertilizer mixture, application, etc., can solve problems affecting product quality, easy to infect miscellaneous bacteria, increase production cost, etc., to improve product quality, reduce production cost, The effect of shortening the fermentation time

Inactive Publication Date: 2009-11-25
嵇书琼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the strains of the compound bacterial agent are generally combined with two or three of them. Since the fermentation cycles of these four bacteria are greatly different, it is difficult to complete the fermentation of the four bacteria at the same time in production, so it is difficult to simultaneously adsorb on the adsorption. agent, and then mix in proportion
The problem is that every single bacterial agent that is prepared first is first packed in a turnover bag, and then mixed in proportion after the other three are produced one after another.
The single bacterial agent produced in this way is easy to be contaminated with bacteria and dry because it is stored in the turnover bag, and it is easy to dry out, which affects the quality of the final product
In addition, each time a type of bacteria is produced, it must be packaged once, and then unpacked, mixed, and repackaged. There are several more processes, which will increase the production cost and production cycle.

Method used

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  • Agricultural composite microbial preparation and preparing method thereof
  • Agricultural composite microbial preparation and preparing method thereof
  • Agricultural composite microbial preparation and preparing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Example 1: Research on the fermentation process conditions of soybean rhizobia, Azotobacter chroococcus, Bacillus megaterium and Bacillus glaci according to the present invention

[0023] 1. Preparation of soybean rhizobia (ACCC15401 or ACCC15400 or ACCC15403) fermentation broth: the fermentation broth is sterilized at 121°C for 30 minutes, and the temperature is reduced to 32-33°C to inoculate the rhizobium seed solution, the inoculation amount is 1%-3%. During the fermentation process, the temperature is controlled at 32-34℃, and the aseptic air volume is controlled at 60-80m 3 / hour. Fermentation end time: 18-24 hours. The formula of fermentation broth: K 2 HPO 4 0.25-0.8g, NaCl 0.1-0.5g, MgSO 4 0.1-0.4g, mannitol 5-20g, yeast powder 0.4-1.5g, CaCO 3 1-5g, 1000mL water, pH 6.8-7. The preparation and sterilization process of seed culture is the same as fermentation culture.

[0024] 2. Preparation of Azotobacter chroococcus (ACCC10098) fermentation broth: the fermentation...

Embodiment 2

[0057] Example 2: Examples of the ratio of soybean rhizobia inoculum, Azotoxin-fixing bacteria inoculum, Bacillus megaterium inoculum and Bacillus coli inoculum of the present invention:

[0058] The ratio of the inoculum can be changed within the following range: the ratio of soybean rhizobia inoculum is 40-70% (weight percentage), the ratio of Azotoxin-fixing bacteria inoculum is 10-20% (weight percentage), and the ratio of Bacillus megaterium is 5-10% (weight percentage), and the proportion of Bacillus glaciens is 15-30% (weight percentage).

[0059] The proportioning method is: the bacteria liquid fermented according to the best fermentation conditions obtained in Example 1 is adsorbed on the adsorbent at a ratio of about 15-20% (weight percentage) (the ratio depends on the moisture content of the adsorbent) ), the adsorbent can use one or more of organic materials such as lignite, vermiculite, grass charcoal, bran, straw or animal manure. The product made of the adsorbent tha...

Embodiment 3

[0113] Example 3: Modification test of the compound microbial agent provided by the present invention on soil nutrients

[0114] Guangshun Chengpeng Orchard is located in Beijing. The soil quality in the garden is relatively poor, especially the content of organic matter is very low. Since 2003, the compound microbial inoculum provided by the present invention has been used (50% soybean rhizobia, Azotobacter chroococcus 20%, Bacillus megaterium 10%, and Bacillus glaciens 20% in proportions). The base fertilizer is applied or used as topdressing, and the ditch is applied to the soil at the root of the crop. In the same year, the orchard began to collect soil samples to analyze the indicators of soil organic matter, total nitrogen, available nitrogen, available phosphorus and available potassium. The results are summarized as follows:

[0115] Table 15: Test results of soil composition

[0116]

[0117] Note: The increase refers to the comparison of the test results in each year w...

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Abstract

The invention discloses an agricultural composite microbial preparation, respective zymocyte solutions of soja bean legume bacteria, round brown azotobacteria, bacillus megaterium and colloidal substance bacillus are respectively adsorbed on the adsorbing agent according to the proportionality of 15-20 % by weight to prepare soja bean legume bacteria, round brown azotobacteria, bacillus megaterium and colloidal substance bacillus, which are compounded to the agricultural composite microbial preparation; the invention adopts the composition of four kinds of bacterial seeds, also considers the requirement ofnitrogen, phosphor and kalium nutrients for plant, it is proved by the field experiment, the obvious yield increasing and quality raising actions for multiple corps are provided; the method accelerates the growth respectively adopting the controls of air flow of microorganism, temperature and inoculating amount during fermentation, enables the fermentation periods of the four kinds of composite bacterial series to be shortened, thereby fermenting at the same time and the terminating at the same time.

Description

Technical field [0001] The invention relates to an agricultural fertilizer, in particular to an agricultural composite microbial inoculum and a preparation method thereof. Background technique [0002] Nitrogen, phosphorus, and potassium are the three essential elements for plant growth. Phosphorus and potassium in the soil are in the form of organic or inorganic phosphorus and potassium compounds. Plants can’t directly absorb and utilize them. Through Bacillus megaterium, which can dissolve phosphorus, and Bacillus glaciens, which dissolve potassium, the phosphorus that is difficult for plants to absorb can be separately absorbed. And potassium is converted into phosphorus and potassium which can be directly used by plants. The molecular nitrogen in the air cannot be absorbed by plants. Through the symbiotic nitrogen fixation of rhizobia and the autogenous nitrogen fixation of Azotobacter chroococcus, the molecular nitrogen is converted into ammonia for direct absorption and uti...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05F11/08A01C21/00
Inventor 嵇书琼
Owner 嵇书琼
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