Method for preserving alternaria alternata

Abstract

The invention discloses a method for preserving alternaria alternate, comprising: field typical brown spot is collected, and germ spores are separated by using a simple single-spore method; bacterial strain with strong pathogenicity can be screened by a detached leaf pendant-drop method, tobacco leaves can be inoculated by being sprayed with spore liquid of the bacterial strain with strong pathogenicity, the inoculated leaves are moisturized and maintained the humidity level for pothogenesy, and the brown spot is dried in the air or parched, and is then sealed and preserved in a refrigerator at 2-4 DEG C; wherein, both the typical brown spot and scab which is taken as the strain to be preserved at the low temperature are applicable. The method adopting host plants for preserving the germs can effectively maintain the pathogenicity of the germs. Fresh spores with stable heredity can be conveniently prepared by being matched with the simple single-spore isolation after the scab is moisturized. The germs can be preserved for at least five years, so that the hereditary stability and the consistency of pathogenicity of the germs can be effectively maintained.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for preserving tobacco red spot fungus. Background technique [0002] Tobacco Brown Spot Disease (Tobacco Brown Spot Disease) is an important leaf disease in the late mature stage of tobacco leaves. It commonly occurs in various smoking areas and is the main disease of tobacco in my country. The pathogen is Alternaria alternata. At present, continuous subculture is usually used for the preservation of tobacco Alternaria spp., but subculture often leads to a decrease in the pathogenicity of the bacteria, and it is also easy to cause changes in other genetic traits, which directly affect the determination of disease resistance of tobacco varieties, screening of biocontrol bacteria, and control. The accuracy and consistency of drug screening are not convenient for monitoring the natural pathogenicity differentiation and variation of field pathogens. So...

AI Technical Summary

Problems solved by technology

However, it is still a preservation method away from the host, and its survival and pathogenicity need to be monitored; in addition, because it is a bacterial layer formed by static culture of Richards culture medium, its sporulation ability of mycelium is not as good as that of the host lesion

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