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White lablab seed extract as well as extracting method and application thereof in preparing ultraviolet injury resistant preparations

An extraction method and anti-ultraviolet technology, which can be used in skin care preparations, pill delivery, pharmaceutical formulations, etc. It can solve the problems of insufficient attention to long-wave ultraviolet rays, difficulty in treatment, and loose skin, so as to achieve anti-ultraviolet radiation effects and reduce or prevent damage. , powerful effect

Active Publication Date: 2009-10-07
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ultraviolet radiation in area A accounts for 98% of the total energy of ultraviolet rays, most of which pass through the dermis, and a small amount of it penetrates the subcutaneous tissue under the dermis. damage
[0006] 2. Ultraviolet radiation damage to the skin
[0007] 2.1 Acute damage caused by ultraviolet radiation: under the sun, the skin is exposed to strong radiation for a long time, if no protective measures are taken, it will lead to acute red shift effect on the skin
[0008] 2.2 Chronic photoaging caused by ultraviolet radiation: long-wave ultraviolet rays have strong transmission power to the skin, can penetrate the epidermis and most of the dermis, destroy the elastic fibers in the skin, make the muscles lose elasticity, cause skin relaxation, and lead to skin photoaging
[0015] Due to technical reasons, most sunscreens are still used for the protection of medium-wave ultraviolet rays, and insufficient attention is paid to long-wave ultraviolet rays
Although the energy of UVA irradiating the human body accounts for 98% of the total energy of ultraviolet rays, the penetration to the skin is much greater than that of UVB. Long-term UVA exposure will cause serious damage to the skin and it is difficult to treat

Method used

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  • White lablab seed extract as well as extracting method and application thereof in preparing ultraviolet injury resistant preparations
  • White lablab seed extract as well as extracting method and application thereof in preparing ultraviolet injury resistant preparations
  • White lablab seed extract as well as extracting method and application thereof in preparing ultraviolet injury resistant preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Embodiment 1, the preparation of white lentil extract

[0083] The method for obtaining the white lentil extract of the present invention comprises the following steps:

[0084] 1) 90g white lentils are mechanically crushed into powder.

[0085] 2) Extract the white lentil powder obtained in step 1) with physiological saline, the method is: mix the white lentil powder with 6 and 8 times the volume (4-10 times the volume can be) of physiological saline respectively, and stir each at room temperature After 3 hours (0.5-6 hours are acceptable), filter with 4-8 layers of gauze, combine two batches of filtrates, then centrifuge at 20,000 rpm for 15 minutes at 4°C, discard the precipitate, and the supernatant is the white lentil extract.

[0086] 3) Add solid (NH 4 ) 2 SO 4 , respectively to (NH 4 ) 2 SO 4 Carry out salting-out after 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100% (W / V) saturation concentration, stand in 4 ℃ refrigerator for 2 hours (1-4 hours average availab...

Embodiment 2

[0088] Embodiment 2, the preparation of white lentil extract

[0089] The method for obtaining the white lentil extract of the present invention comprises the following steps:

[0090] 1) 180g white lentils are mechanically crushed.

[0091] 2) Extract the white lentil powder obtained in step 1) with physiological saline, the method is: mix the white lentil powder with 4, 7 and 10 times the volume (4-10 times the volume can be) of physiological saline respectively, each at room temperature After stirring for 6 hours (0.5-6 hours are acceptable), filter with 4-8 layers of gauze, combine three batches of filtrate, then centrifuge at 20000rpm at 4°C for 25min, discard the precipitate, and the supernatant is the white lentil extract.

[0092] 3) Add solid (NH 4 ) 2 SO 4 , to 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100% (W / V) saturation concentration, carry out salting-out respectively, leave standstill in 4 ℃ of refrigerators for 4 hours (1- 4 hours), and then centrifuged at 25,00...

Embodiment 3

[0094] Embodiment 3, the preparation of white lentil extract

[0095] The method for obtaining the white lentil extract of the present invention comprises the following steps:

[0096] 1) 360g white lentils are mechanically crushed.

[0097] 2) Extract the white lentil powder obtained in step 1) with physiological saline. After stirring at room temperature for 0.5 hours (0.5-6 hours are acceptable), filter with 4-8 layers of gauze, combine four batches of filtrates, then centrifuge at 30,000 rpm at 4°C for 25 minutes, discard the precipitate, and the supernatant is the white lentil extract.

[0098] 3) Add solid (NH 4 ) 2 SO 4 , to 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100% (W / V) saturation concentration, carry out salting-out respectively, leave standstill 1 hour in 4 ℃ of refrigerators (1- 4 hours), and then centrifuged at 30,000 rpm for 10 min at 4°C to collect the respective precipitates.

[0099] 4) Step 3) was saturated with different concentrations (NH 4 ) 2 SO 4 Th...

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Abstract

The invention discloses a white lablab seed extract as well as an extracting method and an application thereof in preparing ultraviolet injury resistant preparations. The white lablab seed extract is prepared by the method that after being crashed, white lablab seeds are extracted with physiological saline; then an obtained extract is salted out with saturated ammonium sulphate; and finally, the extract is desalted and dried. Proved by experiments, the white lablab seed extract has special regulation and control effects on tissue cells and can relieve and stop injuries caused by extrinsic factors. Various dosage forms of ultraviolet injury resistant medicaments and cosmetics prepared by using the white lablab seed extract as an active ingredient have better curative effects and have strong radiation resistant effects on skin, therefore, the white lablab seed extract can be used for preparing the ultraviolet injury resistant medicaments and the ultraviolet injury resistant cosmetics.

Description

technical field [0001] The invention relates to a plant extract and its extraction method and application, in particular to the white lentil extract and its extraction method and its new application, especially the application in the preparation of anti-ultraviolet damage medicine and anti-ultraviolet radiation cosmetics. Background technique [0002] The earth on which human beings live, due to the protection of the atmospheric ozone layer, blocks 99% of the ultraviolet radiation in the sun, so that all creatures on the earth are protected from strong ultraviolet rays. It is the protection of the big protective umbrella of the ozone layer that makes the earth The blue planet is full of vitality and is full of vitality everywhere. Therefore, the ozone layer is regarded as a natural protective barrier for human beings and creatures. [0003] Since the Antarctic ozone hole was first reported in 1985, stratospheric ozone depletion has become one of the global environmental issu...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61K8/97A61K9/00A61K9/06A61K9/08A61K9/10A61K9/20A61K9/48A61K47/32A61K47/36A61K47/38A61P17/16A61Q17/04A61K131/00
Inventor 裴雪涛师伟任祥亮岳文白慈贤南雪施双双陈琳吕洋
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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